In vitro Characterization of Chrysovirus-1-Induced Hypovirulence of Bipolaris maydis

Authors

  • Passanesh SUKPHOPETCH Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
  • San SUWANMANEE Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
  • Potjaman PUMEESAT Department of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok 10600, Thailand
  • Sumate AMPAWONG Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand

DOI:

https://doi.org/10.48048/wjst.2021.6564

Keywords:

Bipolaris maydis, Chrysovirus-1, Hypovirulence, Mycovirus

Abstract

Mycoviruses are viruses that infect fungi. Chrysovirus-1 has been reported as a mycovirus in Bipolaris maydis, which is the plant fungal pathogen for maize, and its infection results in the reduction of crop yield. We aimed to characterize Chrysovirus-1-infected B. maydis in terms of macroscopic morphology, transmission electron microscopy, colony size, biofilm formation, and stress responses focusing on osmotic stress (NaCl), oxidative stress (H2O2), thermotolerance (at 25, 37, and 45 °C), pH (5, 7, and 10), and metal stress (ZnSO4) in comparison with an uninfected strain. Our results demonstrated the presence of viral-like particles under TEM. The colony morphology of the infected strain displayed slight differences as significant delay in colony growth of the Chrysovirus-1-infected strain when compared to the uninfected strain. Moreover, biofilm mass of the infected strain was examined as being lower than that of the uninfected strain. Several stress response tests also demonstrated that the infected strain exhibited higher sensitivity to all stress responses compared to the uninfected strain. Thus, our results suggested that mycoviruses as demonstrated in this study (Chrysovirus-1) can induce the hypovirulence phenomenon in the pathogenic fungi (B. maydis).

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References

M Hollings. Viruses associated with a die-back disease of cultivated mushroom. Nature 1962; 196, 962-5.

SA Ghabrial, JR Caston, D Jiang and ML Nibert. 50-plus years of fungal viruses. Virol. J. 2015; 479, 356-68.

M Son, J Yu and Kook-Hyung Kim. Five questions about mycoviruses. PLoS. Pathog. 2015; 11, e1005172.

R Singh and R Srivastava. Southern corn leaf blight-an important disease of maize: An extension fact sheet. Indian. Res. J. Ext. Edu. 2016; 12, 324-7.

Q Deng, H Wang, C Li, P Li, S Fang, S Yang, F Yan, S Zhang and Z Chen. The complete genomic sequence of a novel alphapartitivirus from Bipolaris maydis, the causal agent of corn southern leaf blight. Arch. Virol. 2017; 162, 2433-6.

J Xie, WM Havens, YH Lin, N Suzuki and SA Ghabrial. The victorivirus Helminthosporium victoriae virus 190S is the primary cause of disease/hypovirulence in its natural host and a heterologous host. Virus Res. 2016; 213, 238-45.

MN Pearson, RE Beever, B Boine and K Arthur. Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol. Plant Pathol. 2009; 10, 115-28.

K Ihrmark, H Johannesson, E Stenstrom and J Stenlid. Transmission of double-stranded RNA in Heterobasidion annosum. Fungal. Genet. Biol. 2002; 36, 147-54.

N Moleleki, SWV Heerden, MJ Wingfield, BD Wingfield and O Preisig. Transfection of Diaporthe perjuncta with Diaporthe RNA virus. Appl. Environ. Microbiol. 2003; 69, 3952-6.

D Rigling and S Prospero. Cryphonectria parasitica, the causal agent of chestnut blight: Invasion history, population biology and disease control. Mol. Plant Pathol. 2018; 19, 7-20.

K Suzaki, Ken-ichi Ikeda, A Sasaki, S Kanematsu, N Matsumoto and K Yoshida. Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa. J. Gen. Plant Pathol. 2005; 71, 161-8.

IP Ahn and YH Lee. A viral double-stranded RNA up regulates the fungal virulence of Nectria radicicola. Mol. Plant Microbe. Interact. 2001; 14, 496-507.

P Singh, S Paul, MR Shivaprakash and A Chakrabarti and AK Ghosh. Stress response in medically important Mucorales. Mycoses 2016; 59, 628-35.

H Wang, C Li, L Cai, S Fang, L Zheng, F Yan, S Zhang and Y Liu. The complete genomic sequence of a novel botybirnavirus isolated from a phytopathogenic Bipolaris maydis. Virus Genes 2018; 54, 733-6.

N Herrero. Identification and sequence determination of a new chrysovirus infecting the entomopathogenic fungus Isaria javanica. Arch. Virol. 2017; 162, 1113-7.

TP Mello, AC Aor, DS Goncalves, SH Seabra, MH Branquinha and ALS Santos. Assessment of biofilm formation by Scedosporium apiospermum, S. aurantiacum, S. minutisporum and Lomentospora prolificans. Biofouling 2016; 32, 737-49.

AM Day, CM Herrero-de-Dios, DM MacCallum, AJP Brown and J Quinn. Stress-induced nuclear accumulation is dispensable for Hog1-dependent gene expression and virulence in a fungal pathogen. Sci. Rep. 2017; 7, 14340.

LP Wang, JJ Jiang, YF Wang, N Hong, F Shang, WX Xu and GP Wang. Hypovirulence of the phytopathogenic fungus Botryosphaeria dothidea: Association with a coinfecting Chrysovirus and a Partitivirus. J. Virol. 2014; 88, 7517-27.

KM Lee, J Yu, M Son, YW Lee and KH Kim. Transmission of Fusarium boothii mycovirus via protoplast fusion causes hypovirulence in other phytopathogenic fungi. PloS One 2011; 6, e21629.

X Yu, B Li, YP Fu, JT Xie, J Cheng, SA Ghabrial, G Li, X Yi and D Jiang. Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proc. Natl. Acad. Sci. USA 2013; 110, 1452-7.

LF Zhai, J Xiang, MX Zhang, M Fu, Z Yang, N Hong and G Wang. Characterization of a novel double-stranded RNA mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea. J. Virol. 2016; 493, 75-85.

R Duran, JW Cary and AM Calvo. Role of the osmotic stress regulatory pathway in morphogenesis and secondary metabolism in filamentous fungi. Toxins 2010; 2, 367-81.

MB Angelova, SB Pashova, BK Spasova, SV Vassilev and LS Slokoska. Oxidative stress response of filamentous fungi induced by hydrogen peroxide and paraquat. Mycologic. Res. 2005; 109, 150-8.

R Abrashev, S Stoitsova, E Krumova, S Pashova, T Paunova-Krasteva, S Vassilev, P Dolashka-Angelova and M Angelova. Temperature-stress tolerance of the fungal strain Aspergillus niger 26: Physiological and ultrastructural changes. World J. Microbiol. Biotechnol. 2014; 30, 1661-8.

D Sardella, R Gatt and VP Valdramidis. Assessing the efficacy of zinc oxide nanoparticles against Penicillium expansum by automated turbidimetric analysis. Mycology 2017; 9, 43-8.

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Published

2021-01-17

How to Cite

SUKPHOPETCH, P. ., SUWANMANEE, S. ., PUMEESAT, P. ., & AMPAWONG, S. . (2021). In vitro Characterization of Chrysovirus-1-Induced Hypovirulence of Bipolaris maydis. Walailak Journal of Science and Technology (WJST), 18(3), Article 6564 (8 pages). https://doi.org/10.48048/wjst.2021.6564