Identification of Growth-related EST-Microsatellite Marker in Nile Tilapia (Oreochromis niloticus)

Sk Md Saeef Ul Hoque Chishty, Suwit Wuthisuthimethavee, Piyapong Chotipuntu, Sataporn Direkbusarakom

Abstract


This study aimed to investigate the growth related EST-microsatellites marker in Nile tilapia. The frequency of allele and genotype of microsatellite marker concentrating on the association between these makers and growth related allele and genotype were studied. Nile tilapia of Thai strain was used. The broodstock were collected from five different fisheries stations in Thailand, Uttaradit, Pathum Thani, Phetchaburi, Buri Ram and Chumphon. The brood fish were reared to spawn and their offspring were reared for 83 days before they were utilized as the experimental samples. Nineteen EST-microsatellite markers were screened with isolated DNA samples considering fast and slow growth. Out of 19 loci, 14 loci namely OMO392, OMO051, OMO097, OMO072, OMO327, OMO277, OMO122, OMO193, OMO198, OMO200, OMO335, OMO374, OMO049, and OMO069 were found to be polymorphic using F1, F11, and F24 families of Chumphon and Petchaburi stations. Another 4 loci i.e. OMO059, OMO068, OMO315, and OMO337 were observed monomorphic. A total of 51 alleles out of 25 alleles of which the estimated allele frequency was above 0.25 whereas 41 alleles was more than 0.1 allele frequency. An association study for polymorphic loci using one way analysis of variance showed significant relationship (P<0.05) in A3 allele of the fast growth group. A substantial association of EST-microsatellite of OMO392 was identified. Besides, genotyping associations were further resulted 79 genotypes on the other hand 19 were estimated as the frequency exceeding 0.25. Correspondingly, no significant (P>0.05) genotypes were related with growth. Overall, association study of identified allele A3 which designates growth hormone related EST-microsatellite primer OMO392 is potential to facilitate marker assisted selection regarding fast growth of Nile tilapia.

Keywords


EST-Microsatellite marker, Oreochromis niloticus, polymorhic loci, allele, genotype

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