In vitro Phytochemical, Larvicidal and Antimicrobial Activities of Gum Arabic Extract


  • Kauther Sir Elkhatim ALI College of Applied and Industrial Sciences, Department of Biotechnology, University of Bahri, Khartoum, Sudan
  • Tanzeel Altaib Ali SALIH College of Applied and Industrial Sciences, Department of Biotechnology, University of Bahri, Khartoum, Sudan
  • Hussien M. DAFFALLA Commission for Biotechnology and Genetic Engineering, National Centre for Research, Khartoum, Sudan



Acacia senegal, Antimicrobial, Culex quinquefasciatus, gum Arabic, secondary metabolites


The Gum Arabic of Acacia senegal (GA) has been reported to treat several diseases, such as kidney failure and cardiovascular and gastrointestinal disease. However, scarce investigation has been made into the phytoconstituents of GA. Obtained GA was macerated in water, then GA aqueous extract was subjected to phytochemical analysis using standard protocols and bioactivity screening by different procedures. Antimicrobial screening was performed using the cup-plate diffusion method against four bacterial strains and one fungi strain. The larvicidal activity was evaluated against the third instar of Culex quinquefasciatus. The phytochemical analysis showed that GA extract contains high amounts of saponins and alkaloids, moderate amounts of cardiac glycosides, and trace amounts of tannins. GA extract exhibited antimicrobial activity against the test organisms, with different zones of inhibition ranging 0 - 18 mm. The larvicidal activity showed significant perfection with increasing extract dose and exposure period with mortality up to 86.7 %. Results reveal that the crude extract of GA contains important biomolecules which has been proved to have substantial larvicidal and antimicrobial activities.


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PA Williams, OHM Idris and GO Phillips. Structural Analysis of Gum from Acacia senegal (Gum Arabic). In: Nothnagel et al. (Eds.). Cell and Developmental Biology of Arabinogalactan-Proteins. Kluwer Academic/Plenum Publishers, 2000, p. 241-51.

WC Evans, D Evans and GE Trease. Trease and Evans pharmacognosy. 16th ed. Saunders/Elsevier, Edinburgh, 2009, p. 616.

R Jain, P Sharma, T Bhagchandani and SC Jain. Phytochemical investigation and antimicrobial activity of Acacia senegal root heartwood. J. Pharm. Res. 2012, 5, 4934-8.

AR Ndhlala, SO Amoo, B Ncube, M Moyo, JJ Nair and JV Staden. Antibacterial, Antifungal, and Antiviral Activities of African Medicinal Plants. Medicinal Plant Research in Africa, Elsevier, 2013, p. 621-59.

SMA Alawi, MA Hossain and AA Abusham. Antimicrobial and cytotoxic comparative study of different extracts of Omani and Sudanese Gum acacia. Beni-Suef Univ. J. Basic Appl. Sci. 2018; 7, 22-6.

ML Saini, R Saini, S Roy and A Kumar. Comparative pharmacognostical and antimicrobial studies of Acacia species (Mimosaceae). J. Med. Plants Res. 2008, 2, 378-86.

TC Baldwin, PE Quaha and AR Menzies. A serotaxonomic study of Acacia gum exudates. Phytochemistry 1999, 50, 599-606.

MA Montenegro, ML Boiero, L Valle and CD Borsarelli. Gum Arabic: More Than an Edible Emulsifier, Products and Applications of Biopolymers. In: J Verbeek (Ed.). InTech, 2012, p. 220.

IA Bnuyan, NL Hindi, MH Jebur and MA Mahdi. In vitro antimicrobial activity of gum Arabic prebiotics against infectious pathogens. Int. J. Pharm. Pharm. Sci. 2015, 3, 77-85.

MMM Banjar, AM Khafaji and YA Maher. Antimicrobial activity of hydrogen peroxide, sesame and gum Arabic against Streptococcus. Int. J. Health Sci. Res. 2017, 7, 97 -104.

RG Marwah, MO Fatope, RA Mahrooqi, GB Varma, HA Abadi and SKS Al-Burtamani. Antioxidant capacity of some edible and wound healing plants in Oman. Food Chem. 2007; 101, 465-70.

S Sasidharan, Y Chen, D Saravanan, KM Sundram and LY Latha. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr. J. Tradit. Compl. Altern. Med. 2011; 8, 1-10.

AJ Harbone. Phytochemical Method: A Guide to Modern Techniques of Plant Analysis. 3rd ed. Springer, Netherlands, 1998, p. 302.

AA Miles and SS Misra. The estimation of the bactericidal power of the blood. J. Hyg. 1938; 38, 732-49.

F Kavanagh. Analytical Microbiology. In: F Kavanagh (Ed.). Academic Press, New York & London, 1972, p. 11.

WHO. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. Communicable Disease Control, Prevention and Eradication; WHO, Pesticide Evaluation Scheme, WHO, Geneva, Switzerland, 2005, p. 1-219.

DJ Finney. Probit Analysis. 3rd (Ed.). Cambridge University Press, London, 1971, p. 20-42.

VSN International Ltd. GenStat Release 10.3. GenStat Discovery Edition 4, Gen Stat Procedure Library Release PL18.2, VSN International Ltd. (Rothamsted Experimental Station), 2011.

SO Okoro, AH Kawo and AH Arzai. Phytochemical screening, antibacterial and toxicological activities of Acacia senegal extracts. Bayero J. Pure Appl. Sci. 2012; 5, 163-170.

IY Suleiman, SA Yaro, M Abdulwahab, SA Salihu and OC Ogheneme. Phytochemical and spectroanalytical characterizations of some plants extract as green corrosion inhibitors. J. Mater. Environ. Sci. 2017; 8, 3423-32.

IB Abdel-Farid, MG Sheded and EA Mohamed. Metabolomic profiling and antioxidant activity of some Acacia species. Saudi J. Biol. Sci. 2014; 21, 400-8.

T Shimada. Salivary proteins as a defense against dietary tannins. J. Chem. Ecol. 2006; 32, 1149-63.

DT Clark, MI Gazi, SW Cox, BM Eley and GF Tinsley. The effects of Acacia arabiea gum on the in vitro growth and protease activities of periodontopathic bacteria. J. Clin. Periodontol. 1993; 20, 238-43.

C Kamaraj, A Abdul Rahuman, A Bagavan, G Elango, AA Zahir and T Santhoshkumar. Larvicidal and repellent activity of medicinal plant extracts from Eastern Ghats of South India against malaria and filariasis vectors. Asian Pac. J. Trop. Med. 2011; 4, 698-705.

P Vivekanandhan, R Venkatesan, G Ramkumar, S Karthi, S Senthil-Nathan and MS Shivakumar. Comparative analysis of major mosquito vectors response to seed-derived essential oil and seed pod-derived extract from Acacia nilotica. Inte. J. Environ. Res. Public Health. 2018; 15, 388.

R Vahitha, MR Venkatachalam, K Murugan and A Jebanesan. Larvicidal efficacy of Pavonia zeylanica L. and Acacia ferruginea D.C. against Culex quinquefasciatus Say. Bioresour. Technol. 2002; 82, 203-4.

A Ghosh, N Chowdhury and G Chandra. Plant extracts as potential mosquito larvicides. Indian J. Med. Res. 2012; 135, 581-98.

KS Ali and HM Daffalla. Physicochemical and functional properties of the gum arabic from Acacia senegal. Ann. Food Sci. Technol. 2018, 19, 27-34.




How to Cite

ALI, K. S. E. ., SALIH, T. A. A. ., & DAFFALLA, H. M. . (2018). In vitro Phytochemical, Larvicidal and Antimicrobial Activities of Gum Arabic Extract. Walailak Journal of Science and Technology (WJST), 17(3), 192–199.