Serotype Distribution and Demographics of Dengue Patients in a Tertiary Hospital of Lahore, Pakistan during the 2011 Epidemic

Authors

  • Muhammad MUNIR Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Riffat MEHBOOB Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Samina NAEEM Department of Pathology, King Edward Medical University, Lahore
  • Muhammad SALMAN Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Shehryar AHMED Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Irshad Hussain QURESHI Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Tahira Murtaza CHEEMA Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Akmal LAEEQ Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Ashraf SULTAN Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Nakhshab CHOUDHRY Department of Pathology, King Edward Medical University, Lahore
  • Asad Aslam KHAN Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore
  • Fridoon Jawad AHMAD Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore

Keywords:

Dengue, serotypes, Pakistan, DEN 2, Lahore, demography, serotype distribution, 2011 epidemic

Abstract

The dengue outbreak in Lahore, Pakistan during 2011 was unprecedented in terms of its severity and magnitude. This research aims to determine the serotype distribution of the dengue virus during this outbreak and classify the patients demographically. 5ml of venous blood was drawn aseptically from 166 patients with dengue like signs to test for the virus between the months of August to November 2011. The samples were sent to CDC, Atlanta, Georgia for the purpose of molecular assays to determine their serotype. RT-PCR protocol was performed targeting the 4 dengue serotypes. Out of 166 cases, dengue infection was detected with RT-PCR in 95 cases, all infected with the same serotype DEN-2. 75 % of positive cases were males while 25 % were females. Most positive patients were in the age range of 16 - 30 years. 33 % positive cases had accompanying bleeding. This is first study during the 2011 dengue epidemic in Lahore that reports DEN-2 as the only prevalent serotype. It also indicates that more infected patients were males, adults, within the age range of 16 - 30 years, the epidemic peaked in the month of November, Dengue hemorrhagic fever (DHF) is manifested more in females, and that Ravi town was heavily hit by the dengue virus infection.

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Author Biography

Fridoon Jawad AHMAD, Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore

Professional Experience:

2005-Present   Visiting Faculty,
Lahore University of Management Sciences, School of Science and Engineering (LUMS-SSE, Lahore, Pakistan.

2005-Present   Foreign faculty professor, Higher Education Commission
King Edward Medical University, Lahore, Pakistan (July 1, 2006-Present).
Center for Excellence in Molecular Biology Lahore, Pakistan (Feb 4,2005-June 30,2006).                                                                                   
2001-2005 Consultant.
Drexel University College of Medicine, Philadelphia
Pennsylvania.

Professional Experience (Continued):

2002-2003  Consultant.
Northwestern University, Medical School Chicago, Illinois.

1998-2000  Scientist.
The Medical School, The University of
Wisconsin-Madison.

1991-1994  Research Specialist.
The Medical School, The University of
Wisconsin-Madison.

Education:
2000-2001  Post Graduate Training in Medical
Cytogenetics, State Laboratory of Hygiene,
Madison Wisconsin.

1994-1998   PhD Cellular and Molecular Biology,
The University of Wisconsin-Madison.

1987-1991   B.S. Genetics,
The University of Wisconsin-Madison.

Professional Memberships:
American Society for Cell Biology
American Society for Human Genetics

Current Grants:
Startup Research Grant for Faculty Hired under Foreign Faculty Program (PKR 1,000,000. Funded)

Development of “Stem Cell Therapy” for Patients Suffering from Heart Disease in Pakistan (PKR 4,106,333. Funded).

Umbilical cord blood Mesenchymal Stem Cells for Deep Burn Wounds: A novel approach in Tissue Engineering:(PKR 20,000,000 Applied).

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Published

2013-03-14

How to Cite

MUNIR, M., MEHBOOB, R., NAEEM, S., SALMAN, M., AHMED, S., QURESHI, I. H., CHEEMA, T. M., LAEEQ, A., SULTAN, A., CHOUDHRY, N., KHAN, A. A., & AHMAD, F. J. (2013). Serotype Distribution and Demographics of Dengue Patients in a Tertiary Hospital of Lahore, Pakistan during the 2011 Epidemic. Walailak Journal of Science and Technology (WJST), 10(3), 305–314. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/528

Issue

Vol. 10 No. 3 (2013)

Section

Research Article

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