Salt Stress-Responsive Protein Interaction in Hordeum vulgare
DOI:
https://doi.org/10.48048/wjst.2020.5029Keywords:
Salinity stress, salt-responsive protein, protein-protein interaction network, barleyAbstract
Salt stress affects crop productivity by altering the biology of the plant and limiting productivity. Hordeum vulgare is the most tolerant cereal crop, with rich genetic resources. The underlying molecular mechanism involved in salt stress response is yet to be comprehensively addressed. A total of 305 proteins are involved in the network. We attempted to find relationships between a few representative stress-responsive proteins of osmotic (pip1), ionic (K+/Na+ ratio in the leaf sheath, HvHAK, HAK4, NHX1 and Ha1), and oxidative stress (APX, CAT1, SOD1) from the public protein database to identify the most influential protein in the network. Further, the salt response proteins were analyzed for their enriched protein domains, Kyoto Encyclopedia of Genes and Genomes pathways, molecular functions, and cell localization. The graph theory analysis of the large data could provide clues for the identification of potential biomarkers for salt stress in barley. An experiment was performed in three accessions of H. vulgare to identify the reliability of the theoretical network relationship in biological systems. The expression of the above-mentioned proteins was further experimentally proven based on the expression and assay.
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E Darko, K Gierczik, O Hudaak, P Forgoa, M Pal, E Turkosi, V Kovacs, S Dulai, I Molnar, T Janda and M Molnar-Lang. Differing metabolic responses to salt stress in wheat-barley addition lines containing different 7H chromosomal fragments. PLoS One 2017; 12, e0174170.
National Center for Biotechnology Information, Available at: http://www.ncbi.nlm.nih.gov, accessed December 2017.
Y Nakano and K Asada. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981; 22, 867-80.
U Deinlein, AB Stephan, T Horie, W Luo, G Xu and JI Schroeder. Plant salt-tolerance mechanisms. Trends Plant Sci. 2014; 196, 371-9.
E Maas and J Poss. Salt sensitivity of cowpea at various growth stages. Irrig. Sci. 1989; 10, 313-20.
C Foyer, H Lopez-Delgado, J Dat and I Scott. Hydrogen peroxide and glutathione associated mechanisms of acclimatory stress tolerance and signaling. Physiol. Plant 1997; 100, 241-54.
B Halliwell and J Gutteridge, Free Radicals in Biology and Medicine. 3rd ed. Oxford University Press, New York, 1999, p. 936-7.
Y Wang, G Qu, H Li, Y Wu, C Wang, G Liu and C Yang. Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii. Mol. Biol. Rep. 2010; 37, 1119-24.
DC Dulahently and J Yates. Protein identification using 2D-LC-MS/MS. Methods 2005; 35, 248-55.
MC Shelden and U Roessner. Advances in functional genomics for investigating salinity stress tolerance mechanisms in cereals. Front. Plant Sci. 2013; 4, 123.
AU Dekoum, VM Assaha, H Saneoka, R Al-Yahyai and MW Yaish. The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Front. Physiol. 2017; 8, 509.
R Hove, M Ziemann and M Bhave. Identification and expression analysis of the barley (Hordeum vulgare L.) aquaporin gene family. PLoS One 2015; 10, e0128025.
F Berteli, E Corrales, C Guerrero, M J Ariza, F Pliego and V Valpuesta. Salt stress increases ferredoxin-dependent glutamate synthase activity and protein level in the leaves of tomato. Physiol. Plantarum 1995; 93, 259-64.
GenomeNet, Available at: http://www.genome.jp, accessed December 2017.
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