Inhibitory Activity of Cold Atmospheric Plasma on Candida albicans

Phenphichar WANACHANTARARAK; Pradoong SUANPOOT; Mudtorlep NISOA

doi:10.48048/wjst.2019.4811

Authors

Phenphichar WANACHANTARARAK Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200
Pradoong SUANPOOT Faculty of Science, Maejo University Phrae Campus, Maejo University, Chiang Mai 54140
Mudtorlep NISOA School of Science, Walailak University, Nakhon Si Thammarat 80161

DOI:

https://doi.org/10.48048/wjst.2019.4811

Keywords:

MTT assay, Candida albican, Cold Atmospheric Plasma

Abstract

Cold Atmospheric Plasma (CAP) application of dental treatment technologies has recently gained much interest. However, information regarding safety, applications, and how they work is limited. In this study, we examined the inhibitory activity of CAP against Candida albican. The Ar gas has been discharged by CAP of 100 KHz and has been produced inside 4 L/min. The system was operated with the HVRF power input 20 W and treatments of 0, 5, 10, 15 min for samples. The Candida albican standard strain used in this experiment was ATCC 10231. In order to assess the inhibitory activity of CAP against Candida albican, we employed 2 methods; the colony number count and MTT colorimetric assay. The colonies of Candida albican standard strain ATCC 10231 were significantly at 5, 10 and 15 min exposure of CAP (p < 0.05) as compared to un-treatment. According to the result of MTT colorimetric assay, we found that cell viability was significant after 10 min exposure as compared to un-treatment (p < 0.05). In summary, the overall results stated that CAP possesses the inhibitory activity against Candida albican standard strain ATCC 10231 at 100 KHz, 20 W after 5 min.

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Author Biography

Phenphichar WANACHANTARARAK, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200

Dental Research Center, Faculty of Dentistry, Chiang Mai University

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