Inhibitory Activity of Cold Atmospheric Plasma on Candida albicans

Phenphichar WANACHANTARARAK, Pradoong SUANPOOT, Mudtorlep NISOA

Abstract


Cold Atmospheric Plasma (CAP) application of dental treatment technologies has recently gained much interest. However, information regarding safety, applications, and how they work is limited. In this study, we examined the inhibitory activity of CAP against Candida albican. The Ar gas has been discharged by CAP of 100 KHz and has been produced inside 4 L/min. The system was operated with the HVRF power input 20 W and treatments of 0, 5, 10, 15 min for samples. The Candida albican standard strain used in this experiment was ATCC 10231. In order to assess the inhibitory activity of CAP against Candida albican, we employed 2 methods; the colony number count and MTT colorimetric assay. The colonies of Candida albican standard strain ATCC 10231 were significantly at 5, 10 and 15 min exposure of CAP (p < 0.05) as compared to un-treatment. According to the result of MTT colorimetric assay, we found that cell viability was significant after 10 min exposure as compared to un-treatment (p < 0.05). In summary, the overall results stated that CAP possesses the inhibitory activity against Candida albican standard strain ATCC 10231 at 100 KHz, 20 W after 5 min.

Keywords


MTT assay, Candida albican, Cold Atmospheric Plasma

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References


DR Soll. Candida albicans. In: PJ Szaniszlo and JL Harris (eds.). Fungal Dimorphism. Plenum Press, New York, 1985, p. 167-95.

ID Jacobsen, D Wilson, B Wächtler, S Brunke, JR Naglik and B Hube. Candida albicans dimorphism as a therapeutic target. Exp. Rev. Anti-Infect. Ther. 2012; 10, 85-93.

J Chandra, DM Kuhn, PK Mukherjee, LL Hoyer, T McCormick and MA Ghannoum. Biofilm formation by the fungal pathogen Candida albicans: Development, architecture, and drug resistance. J. Bacteriol. 2001; 183, 5385-94.

SP Hawser and LJ Douglas. Resistance of Candida albicans biofilms to antifungal agents in vitro. Antimicrob. Agents Chemother.1995; 39, 2128-31.

J Chandra, PK Mukherjee, SD Leidich, FF Faddoul, LL Hoyer, LJ Douglas and MA Ghannoum. Antifungal resistance of candidal biofilms formed on denture acrylic in vitro. J. Dent. Res. 2001; 80, 903-8.

DM Kuhn, T George, J Chandra, PK Mukherjee and MA Ghannoum. Antifungal susceptibility of Candida biofilms: Unique efficacy of amphotericin B lipid formulations and echinocandins. Antimicrob. Agents Chemother. 2002; 46, 1773-80.

MG Shepherd, RTM Poulter and PA Sullivan. Candida albicans: Biology, genetics, and pathogenicity. Ann. Rev. Microbiol. 1985; 39, 579-614.

F Chandler and J Watts. Mycotic, Actinomycotic, and Algal Infections. In: J Kissane (ed.). Anderson’s Pathology. 9th ed. CV. Mosby, St. Louis, 1996, p. 391-432.

KAG Lane. The Merck Manual of Diagnosis and Therapy. Merck Research Laboratories, Whitehouse Station, New Jersey, USA, 1996.

BC Webb, CJ Thomas, MD Willcox, DW Harty and KW Knox. Candida-associated denture stomatitis. Aetiology and management: A review: Part 1 Factors influencing distribution of Candida species in the oral cavity. Aust. Dent. J. 1998; 43, 45-50.

LR Cahn. The denture sore mouth. Ann. Dent. 1936; 3, 33-6.

E Budtz-Jorgensen, A Stenderup and M Grabowski. An epidemiologic study of yeasts in elderly denture wearers. Comm. Dent. Oral 1975; 3, 115-9.

RP Santarpia III, RP Renner, JJ Pollock, AJ Gwinnet and EC Brandt. Model system for the in vitro testing of a synthetic histidine peptide against candida species grown directly on the denture surface of patients with denture stomatitis. J. Prosthet. Dent. 1988; 60, 62-70.

RP Santarpia III, JJ Pollock, RP Renner and E Spiechowicz. An in vivo replica method for the site-specific detection of Candida albicans on the denture surface in denture stomatitis patients: Correlation with clinical disease. J. Prosthet. Dent. 1990; 63, 437-43.

DR Radford, SJ Challacombe and JD Walter. Denture plaque and adherence of Candida albicans to denture base materials in vivo and in vitro. Crit. Rev. Oral Bio. M 1999; 10, 99-116.

C Hoshing, S Dixit, A Mootha and N Diwan. Role of Candida albicans in denture stomatitis. J. Indian Acad. Oral Med. Radiol. 2011; 23, 617-9.

M Petrovic, M Kostic, M Kostic, N Krunic, M Igic, ZU Pešić and S Otašević. Therapeutic alternatives of natural compounds in treatment of Candida-associated denture stomatitis. Acta Med. Medianae 2014; 53, 73-9.

E Budtz-Jørgensen. Materials and methods for cleaning dentures. J. Prosthet. Dent. 1979; 42, 619-23.

S Hahnel, M Rosentritt, R Burgers, G Handel and R Lang. Candida albicans biofilm formation on soft denture liners and efficacy of cleaning protocols. Gerodontology 2012; 29, 383-91.

JB Huh, Y Lim, HI Youn, BM Chang, JY Lee and Shin SW. Effect of denture cleansers on Candida albicans biofilm formation over resilient liners. J. Adv. Prosthodont. 2014; 6, 109-14.

EM Lima, JS Moura, AADB Cury, RC Garcia and JA Cury. Effect of enzymatic and NaOCl treatments on acrylic roughness and on biofilm accumulation. J. Oral Rehabil. 2006; 33, 356-62.

C Hoffmann, C Berganza and J Zhang. Cold atmospheric plasma: methods of production and application in dentistry and oncology. Med. Gas. Res. 2013; 3: 21.

SA Ermolaeva, AF Varfolomeev, MY Chernukha, DS Yurov, MM Vasiliev, AA Kaminskaya, MM Moisenovich, JM Romanova, AN Murashev, II Selezneva, T Shimizu, EV Sysolyatina, IA Shaginyan, OF Petrov, EI Mayevsky, VE Fortov, GE Morfill, BS Naroditsky and AL Gintsburg. Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds. J. Med. Microbiol. 2011; 60, 75-83.

I Koban, R Matthes, NO Hübner, A Welk and P Meisel. Treatment of Candida albicans biofilms with low-temperature plasma induced by dielectric barrier discharge and atmospheric pressure plasma jet. New J. Phys. 2010; 12, 1-15.

T Maisch, T Shimizu, G Isbary, J Heinlin, S Karrer, TG Klämpfl, YF Li, G Morfill and L Julia. Contact-free inactivation of Candida albicans biofilms by cold atmospheric air plasma. Appl. Environ. Microb. 2012; 78, 4242-7.

N Rahimi-Verki, A Shapoorzadeh, M Razzaghi-Abyaneh, SM Atyabi, M Shams-Ghahfarokhi, Z Jahanshiri and M Gholami-Shabani. Cold atmospheric plasma inhibits the growth of Candida albicans by affecting ergosterol biosynthesis and suppresses the fungal virulence factors in vitro. Photodiagn. Photodyn. 2016; 13, 66-72.

F Sohbatzadeh, AH Colagar, S Mirzanejhad, S Hajiahmadi, M Talebzadeh and M Ghasemi. Investigation of cold atmospheric afterglow plasma effect on Candida albicans fungus. In: Proceedings of the Iran Physics Conference, Iran, 2011, p. 52.

J McFarland. The nephelometer: An instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J. Am. Med. Assoc. 1907; 14, 1176-8.

MV Berridge and AS Tan. Characterisation of the cellular reduction of 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 1993; 303, 474-82.

K Fricke, I Koban, H Tresp, L Jablonowski, K Schröder, A Kramer, KD Weltmann, TV Woedtke and T Kocher. Atmospheric pressure plasma: A high-performance tool for the efficient removal of biofilms. PLoS One 2012; 7, e42539.

R Matthes, L Jablonowski, I Koban, A Quade, NO Hübner, R Schlueter, KD Weltmann, TV Woedtke, A Kramer and T Kocher. In vitro treatment of Candida albicans biofilms on denture base material with volume dielectric barrier discharge plasma (VDBD) compared with common chemical antiseptics. Clin. Oral Invest. 2015; 19, 2319-26.

R Matthes, C Bender, R Schlüter, I Koban, R Bussiahn, S Reuter, J Lademann, KD Weltmann and A Kramer. Antimicrobial efficacy of two surface barrier discharges with air plasma against in vitro biofilms. PLoS One 2013; 8, e70462.

SM Levitz and RD Diamond. A rapid colorimetric assay of fungal viability with the tetrazolium salt MTT. J. Infect. Dis. 1985; 152, 938-45.

B Jahn, E Martin, A Stueben and S Bhakdi. Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. J. Clin. Microb. 1995; 33, 661-7.


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