Phytochemical Constituents and Cholinesterase Inhibitory Activities of Millingtonia hortensis


  • Warachate KHOBJAI Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130
  • Orapit SUTHIVATTANAKUL Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130
  • Somsiri PANUWATTANAWONG Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130



Acetylcholinesterase, cholinesterase inhibitors, Millingtonia hortensis, phytochemical constituents, GC-MS


Acetylcholine (ACh) is an important neurotransmitter in the human brain and nervous system. Acetylcholinesterase inhibitors (AChEIs) are commonly used to improve cognitive function and exist in many plants, including Millingtonia hortensis. M. hortensis, a Thai medicinal plant, has been used as a smoke delivered bronchodilator. The aims of this study were to identify phytochemical constituents and evaluate the cholinesterase inhibitory activity of M. hortensis leaves and flower extracts. The phytochemical identifications were performed by gas chromatography-mass spectrometry. The inhibitory activities of acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) were measured through kinetic enzyme analysis by Ellman’s method. The results showed that all the extracts exhibited specific AChEIs less than 30 % inhibition. While only the chloroform leaf extract inhibited BChE below 30 % inhibitory activities at 5.0 mg/mL. The GC-MS fingerprints revealed 15 main phytochemical constituents in the crude extracts. Additionally, all plant extracts showed antioxidant activity. The leaf extracts were non-poisonous when AChE activity is decreased by 20 - 30 % compared to normal AChE activity.


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Author Biography

Warachate KHOBJAI, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani 12130

Thai Traditional Medicine College


ST DeKosky, RE Harbaugh, FA Schmitt, RAE Bakay, HC Chui, DS Knopman, TM Reeder, AG Shetter, HJ Senter and WR Markesbery. Cortical biopsy in Alzheimer's disease: Diagnostic accuracy and neurochemical, neuropathological, and cognitive correlations. Ann. Neurol. 1992; 32, 625-32.

BG Katzung. Basic and Clinical Pharmacology. The McGraw Hill Companies, Columbus USA, 2001, p. 75-91.

IA Owokotomoa, O Ekundayob, TG Abayomic and AV Chukwukad. In vitro anti-cholinesterase activity of essential oil from four tropical medicinal plants. Toxicol. Rep. 2015; 2, 850-7.

P Taylor and Z Radic. The cholinesterases: From genes to proteins. Annu. Rev. Pharmacol. 1994; 34: 281-320.

I Orhan, B Sener, MI Choudhary and A Khalid. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J. Ethnopharmacol. 2004; 91: 57-60.

M Pohanka. Alzheimer's disease and related neurodegenerative disorders: Implication and counteracting of melatonin. J. Appl. Biomed. 2011; 9, 185-96.

NH Greig, DK Lahiri, and K Sambamurti. Butyrylcholinesterase: An important new target in Alzheimer's disease therapy. Int. Psychogeriatr. 2002; 14, 77-91.

J Minic, A Chatonnet, E Krejci and J Molgo. Butyrylcholinesterase and acetylcholinesterase activity and quantal transmitter release at normal and acetylcholinesterase knockout mouse neuromuscular junctions. Brit. J. Pharmacol. 2003; 138, 177-87.

K Anulakanapakorn, N Bunyapraphatsara and J Satayavivad. Phytochemical and pharmacological studies of the flowers of Millingtonia hortensis linn. f. J. Sci. Soc. Thailand 1987; 13, 71-83.

P Moonkarndi, N Bunyapraphatsara, V Srisukh and H Wagner. The inhibitory activity in 5-lipoxygenase pathway of hispudulin from Millingtonia hortensis L. J. Sci. Soc. Thailand 1991; 17, 51-6.

R Ramasubramaniaraja. Millingtonia hortensis linn.: An overview. Int. J. Pharm. Sci. Res. 2010; 4, 123-5.

M Sharma, S Puri and PD Sharma. Antifungal activity of Millingtonia hortensis. Indian J. Pharm. Sci. 2007; 69, 599-601.

R Kaushik and P Saini. Larvicidal activity of leaf extract of Millingtonia hortensis (Family: Bignoniaceae) against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. J. Vector Borne Dis. 2008; 45, 66-9.

R Kaushik and P Saini. Screening of some semiarid region plants for larvicidal activity against Aedes aegypti mosquitoes. J. Vector Borne Dis. 2009; 46, 244-6.

A Jetty and DS Iyengar. Antimicrobial activity of Millingtonia hortensis leaf extract. Pharm. Biol. 2000; 38, 157-60.

M Chulasiri, N Bunyapraphatsara and P Moongkarndi. Mutagenicity and antimutagenicity of hispidulin and hortensin, the flavonoids from Millingtonia hortensis L. Environ. Mol. Mutagen. 1992; 20, 307-12.

S Tansuwanwong, H Yamamoto, K Imai and U Vinitketkumnuen. Antiproliferation and apoptosis on RKO colon cancer by Millingtonia hortensis. Plant Foods Hum. Nutr. 2009; 64, 11-7.

P Loypimai, A Moongngam and P Chottanom. Effects of ohmic heating on lipase activity, bioactive compounds and antioxidant activity of rice bran. Aust. J. Basic Appl. Sci. 2009; 3, 3642-52.

W Brand-Williams, ME Cuvelier and C Berset. Use of a free radical method to evaluate antioxidant activity. LWT: Food Sci. Tech. 1995; 28: 25-30.

SK Mohanty, K Malappa, A Godavarthi, B Subbanarasiman and A Maniyam. Evaluation of antioxidant, in vitro cytotoxicity of micropropagated and naturally grown plants of Leptadenia reticulate (Retz) Wight & Arn. An endangered medicinal plant. Asian Pac. J. Trop. Med. 2014; 7, 267-71.

GL Ellman, KD Courtney, V Andres and RM Featherstone. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961; 7, 88-95.

HY Choi, EJ Jhun and BO Lim. Application of flow injection-chemiluminescence to the study of radical scavenging activity in plants. Phytother. Res. 2000; 14, 250-3.

BP Doctor, L Toker, E Roth and I Silman. Microtiter assay for acetylcholinesterase. Anal. Biochem. 1987; 166, 399-403.




How to Cite

KHOBJAI, W., SUTHIVATTANAKUL, O., & PANUWATTANAWONG, S. (2017). Phytochemical Constituents and Cholinesterase Inhibitory Activities of Millingtonia hortensis. Walailak Journal of Science and Technology (WJST), 15(8), 589–597.