Enhancement of the Enzymatic and Non-Enzymatic Antioxidant Activities of Cultured Sauropus androgynus L. Merr. Shoots Treated with UV-C Irradiation

Ratchanee Petchang

Abstract


The stimulation of enzymatic and non-enzymatic antioxidant activities of Sauropus androgynus L. Merr. shoot cultures treated with UV-C irradiation was investigated. The shoots were first cultured on Murashige and Skoog (MS) medium containing 1 mg/L 6-benzyladenine (BA) for six weeks. Multiple shoot formations were then treated with UV-C irradiation for 0 min (control group), 5, 10 min (experimental groups) and wild type. After three weeks of culture the shoots were extracted with methanol and analyzed for enzymatic antioxidant activities (with superoxide dismutase; SOD and peroxidase; POD) and non-enzymatic antioxidant activities (with DPPH, ABTS and FRP assays), total phenolic and flavonoid compound contents. The result indicate that the shoot treated with UV-C irradiation for 5 min exhibited the highest enzymatic antioxidant activities (SOD and POD values of 2.26, 2.37 unit/mg proteins, respectively). Non-enzymatic antioxidant activities determined by using DPPH, ABTS and FRP assays was found to very form IC50 values were 10.13 mg/mL, 166.48 mg TEAC/100 g fresh weight (FW) and 22.72 mM FeSO4 /100 g FW, respectively. Total phenolic and flavonoid compound contents had values at 15.54 mg GAE/100 g FW and 63.64 mg QE/100 g FW, respectively. Antioxidant activities revealed a positive moderate high correlation (r2 = 0.729 - 0.980).

Keywords


Sauropus androgynus L. Merr., enzymatic and non-enzymatic antioxidant activities, UV-C irradiation, tissue culture

References


M Suttajit, R Banjerdpongchai, P Kukongviriyapan, S Siriamornpun, C Chaiyasut, S Porasuphatana, U Panit, C Thephinlap and C Charoeansin. Free Radical and Antioxidants. Innovation Health Publisher, Chiangmai, Thailand, 2012.

U Arivalagan, P Alderson and A Nagarajan. Effect of growth hormones on callus induction of Sauropus androgynus (Sweet shoot). Ann. Biol. Res. 2012; 3, 4668-74.

N Benjapak, P Swatsitang, T Senawong and S Tanpanich. Antioxidant capacity of Pak-Wanban (Sauropus androgynus L. Merr.) in human embryonic kidney 293 cells (HEK-293 cells). Agric. Sci. J. 2008; 39, 197-200.

G Nahak and R Sahu. Free radical scavenging activity of multi-vitamin plant (Sauropus androgynus L., Merr.), Available from: http//www.sciencepub.net/researcher, accessed June 2017.

LS Wei, W Wee, JYF Siong and DF Syamsumir. Characterization of antimicrobial, antioxidant, anticancer properties and chemical composition of Sauropus androgynus stem extract. Acta Medica Lituanica 2011; 18, 12-6.

S Padma and D Tejavathi. Role of plant tissue cultures in conservation and reintroduction of Sauropus androgynus L., Merr. In: Proceedings of the 12th of Euro Biotechnology Congress of Spain, Alicante, Spain, 2016.

A Filova. Production of secondary metabolite in plant tissue cultures. Res. J. Agric. Sci. 2014; 46, 236-45.

T Scheper, S Belkin, T Bley, J Bohlmann, B Gu, S Hu, B Mattiasson, J Nielsen, H Seitz, R Ulber, P Zeng, J Zhong and W Zhou. Production of secondary metabolites using plant cell cultures. Adv. Biochem. Eng. Biotech. 2008; 111, 187-228.

T Pumchaosuan and S Wongroung. 2008, Tissue Culture of Ceylon Spinach (Basella rubra Linn.) for Anthocyanin Production. Master of Sciences Thesis in Biotechnology, Chiang Mai University, Thailand.

E Cetin. Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Okuzgozu callus cultures. Biol. Res. 2014; 47, 37-47.

R Petchang. Propagation of some local plants by tissue culture in Tumbon Nanokkok, Lablae, Uttaradit. Naresuan Univ. J. 2011; 19, 35-42.

W Polsak. 2003, In vitro Culture of Aglaia odorata Lour. and Comparison of Secondary Metabolite Production in vitro and in vivo Culture. Master of Science Thesis in Botany, Kasetsart University, Thailand.

O Suriyaphan and S Matchachip. 2009, Effect of Plant Growth Regulators on Free Radical Scavenging Activities and Phenolic Content of Extracts from Selected Medicinal Plant Celli Cultured in vitro. Master of Science, Burapha University, Thailand.

N Benhamou. Elicitor-induced plant defense pathways. Trends Plant Sci. 1996; 1, 233-40.

M Erkan, Y Wang and C Wang. Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in Strawberry. Postharvest Biol. Tech. 2008; 48, 163-71.

N Mohammadi, S Mohammadi, V Abdossi and B Mohammadi. Effect of UV-Cradiation on antioxidant enzymes in Strawberry fruit (Fragaria x ananassa cv. Camarosa). J. Agric. Biol. Sci. 2012; 7, 860-4.

C Solís, C Salazar, E Gloria, O Aguilera, H Zavala, V Campos, V Vázquez and J Emiliano. Ultraviolet light-C increases antioxidant capacity of the strawberry (Fragaria x ananassa) in vitro and in high-fat diet-induced obese rats. Food Sci. Nutr. 2017, DOI: 10.1002/fsn3.487.

M Erkan, Y Wang and C Wang. Effect of UV illumination on antioxidant capacity and postharvest decay in blueberry fruit. J. Food Sci. 2013; 11, 434-6.

C Burana and S Srilaong. Effect of UV-C irradiation on antioxidant activities in Chinese kale (Brassica oleracea var. alboglabra). Agric. Sci. 2009; 40, 137-40.

AEA Mona, R Kawthar and M Hossam. The effect of UV-C on secondary metabolites production of Echinacea purpurea culture in vitro. Biol. Chem. Environ. Sci. 2016; 11, 465-83

J Christine and JC Weydert. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nature Protocols 2010; 5, 51-66.

B Vongsak, P Sithisarn, S Mangmool, S Thongpraditchote, Y Wongkrajang and W Gritsanapan. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind. Crop Prod. 2013; 44, 566-71.

B Arnao, A Cano and M Acosta. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem. 2001; 73, 239-44.

R Ferreira, P Baptista, M Vilas-Boas and L Barros. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem. 2007; 100, 1511-6.

W Chien, C Chi and W Shiow. Changes of flavonoid content and antioxidantcapacity in blueberries after illumination with UV-C. Food Chem. 2009; 117, 426-31

M Dulce, P Rivera, G Alfonso, Y Elhadi, M Miguel, G Gustavo. Effect of UV-C irradiation and low temperature storage on bioactive compounds, antioxidant enzymes and radical cavenging activity of papaya fruit. J. Food Sci. Tech. 2014; 51, 3821-9.

N Pongprasert, S Yoshihiko, S Sumiko and G Hiroshi. The Effect of UV-C treatment on reduction of cellular oxidative stress and the consequential chilling injury symptom of ‘Hom’ banana fruit. Agric. Sci. 2011; 42, 208-11.


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