Enhancement of the Enzymatic and Non-Enzymatic Antioxidant Activities of Cultured Sauropus androgynus L. Merr. Shoots Treated with UV-C Irradiation

Authors

  • Ratchanee PETCHANG Department of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit 53000

DOI:

https://doi.org/10.48048/wjst.2019.4391

Keywords:

Sauropus androgynus L. Merr., enzymatic and non-enzymatic antioxidant activities, UV-C irradiation, tissue culture

Abstract

The stimulation of enzymatic and non-enzymatic antioxidant activities of Sauropus androgynus L. Merr. shoot cultures treated with UV-C irradiation was investigated. The shoots were first cultured on Murashige and Skoog (MS) medium containing 1 mg/L 6-benzyladenine (BA) for 6 weeks. Multiple shoot formations were then treated with UV-C irradiation for 0 min (control group), 5, 10 min (experimental groups), and cultivated field. After 3 weeks of culture the shoots were extracted with methanol and analyzed for enzymatic antioxidant activities (with superoxide dismutase; SOD and glutathione peroxidase; GPX) and non-enzymatic antioxidant activities (with DPPH, ABTS and FRP assays) and total phenolic and flavonoid compound contents. The result indicate that the shoot treated with UV-C irradiation for 5 min exhibited the highest enzymatic antioxidant activities (SOD and GPX values of 2.26, 2.37 unit/mg soluble proteins, respectively). Non-enzymatic antioxidant activities determined by using DPPH, ABTS and FRP assays were found to be IC50 values of 10.13 mg/mL, 166.48 mg TEAC/100 g fresh weight (FW), and 22.72 mM FeSO4 /100 g FW, respectively. Total phenolic and flavonoid compound contents had values at 15.54 mg GAE/100 g FW and 63.64 mg QE/100 g FW, respectively. Antioxidant activities revealed a positive moderate high correlation (r2 = 0.729 - 0.980).

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References

M Suttajit, R Banjerdpongchai, P Kukongviriyapan, S Siriamornpun, C Chaiyasut, S Porasuphatana, U Panit, C Thephinlap and C Charoeansin. Free Radical and Antioxidants. Innovation Health Publisher, Chiangmai, Thailand, 2012, p. 17-294.

D Ferro, R Bakiu, C De Pittà, F Boldrin, F Cattalini, S Pucciarelli, C Miceli, G Santovito. Cu, Zn superoxide dismutases from Tetrahymena thermophila: Molecular evolution and gene expression of the first line of antioxidant defenses. Protist 2015; 166, 131-45.

U Arivalagan, P Alderson and A Nagarajan. Effect of growth hormones on callus induction of Sauropus androgynus (Sweet shoot). Ann. Biol. Res. 2012; 3, 68-74.

N Benjapak, P Swatsitang, T Senawong and S Tanpanich. Antioxidant capacity of Pak-Wanban (Sauropus androgynus L. Merr.) in human embryonic kidney 293 cells (HEK-293 cells). Agr. Sci. J. 2008; 39, 197-200.

G Nahak and R Sahu. Free radical scavenging activity of multi-vitamin plant (Sauropus androgynus L., Merr.). Researcher 2010; 2, 5-10.

LS Wei, W Wee, JYF Siong and DF Syamsumir. Characterization of antimicrobial, antioxidant, anticancer properties and chemical composition of Sauropus androgynus stem extract. Acta Med. Lituanica 2011; 18, 12-6.

S Padma and D Tejavathi. Role of plant tissue cultures in conservation and reintroduction of Sauropus androgynus L., Merr. In: Proceedings of the 12th of Euro Biotechnology Congress of Spain, Alicante, Spain, 2016.

A Filova. Production of secondary metabolite in plant tissue cultures. Res. J. Agr. Sci. 2014; 46, 236-45.

T Scheper, S Belkin, T Bley, J Bohlmann, B Gu, S Hu, B Mattiasson, J Nielsen, H Seitz, R Ulber, P Zeng, J Zhong and W Zhou. Production of secondary metabolites using plant cell cultures. Adv. Biochem. Eng. Biotech. 2008; 111, 187-228.

E Cetin. Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Okuzgozu callus cultures. Biol. Res. 2014; 47, 37-47.

R Petchang. Propagation of some local plants by tissue culture in Tumbon Nanokkok, Lablae, Uttaradit. Naresuan Univ. J. 2011; 19, 35-42.

W Polsak. 2003, In vitro Culture of Aglaia odorata Lour. and Comparison of Secondary Metabolite Production in vitro and in vivo Culture. Master of Science, Thesis in Botany, Kasetsart University, Thailand.

O Suriyaphan and S Matchachip. 2009, Effect of Plant Growth Regulators on Free Radical Scavenging Activities and Phenolic Content of Extracts from Selected Medicinal Plant Celli Cultured in vitro. Master of Science, Burapha University, Thailand.

N Benhamou. Elicitor-induced plant defense pathways. Trends Plant Sci. 1996; 1, 233-40.

M Erkan, Y Wang and C Wang. Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in Strawberry. Postharvest Biol. Tech. 2008; 48, 163-71.

N Mohammadi, S Mohammadi, V Abdossi and B Mohammadi. Effect of UV-C radiation on antioxidant enzymes in Strawberry fruit (Fragaria  ananassa cv. Camarosa). J. Agr. Biol. Sci. 2012; 7, 860-4.

C Solís, C Salazar, E Gloria, O Aguilera, H Zavala, V Campos, V Vázquez and J Emiliano. Ultraviolet light-C increases antioxidant capacity of the strawberry (Fragaria  ananassa) in vitro and in high-fat diet-induced obese rats. Food Sci. Nutr. 2017; 5, 1004-14.

M Erkan, Y Wang and C Wang. Effect of UV illumination on antioxidant capacity and postharvest decay in blueberry fruit. J. Food Sci. 2013; 11, 434-6.

C Burana and S Srilaong. Effect of UV-C irradiation on antioxidant activities in Chinese kale (Brassica oleracea var. alboglabra). Agr. Sci. 2009; 40, 137-40.

AEA Mona, R Kawthar and M Hossam. The effect of UV-C on secondary metabolites production of Echinacea purpurea culture in vitro. Biol. Chem. Environ. Sci. 2016; 11, 465-83.

JC Weydert and JJ Cullen. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat. Protocol. 2010; 5, 51-66.

B Vongsak, P Sithisarn, S Mangmool, S Thongpraditchote, Y Wongkrajang and W Gritsanapan. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind. Crop Prod. 2013; 44, 566-71.

B Arnao, A Cano and M Acosta. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem. 2001; 73, 239-44.

R Ferreira, P Baptista, M Vilas-Boas and L Barros. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem. 2007; 100, 1511-6.

W Chien, C Chi and W Shiow. Changes of flavonoid content and antioxidantcapacity in blueberries after illumination with UV-C. Food Chem. 2009; 117, 426-31.

M Dulce, P Rivera, G Alfonso, Y Elhadi, M Miguel and G Gustavo. Effect of UV-C irradiation and low temperature storage on bioactive compounds, antioxidant enzymes and radical scavenging activity of papaya fruit. J. Food Sci. Tech. 2014; 51, 3821-9.

N Pongprasert, S Yoshihiko, S Sumiko and G Hiroshi. The effect of UV-C treatment on reduction of cellular oxidative stress and the consequential chilling injury symptom of ‘Hom’ banana fruit. Agr. Sci. 2011; 42, 208-11.

L Menolli, F Finger, M Barbosa, T Correia and L Vieira. Peroxidase activity in roots of arracacha affected by pH and temperature. Acta Sci. Agron. 2011; 33, 513-8.

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Published

2018-01-28

How to Cite

PETCHANG, R. (2018). Enhancement of the Enzymatic and Non-Enzymatic Antioxidant Activities of Cultured Sauropus androgynus L. Merr. Shoots Treated with UV-C Irradiation. Walailak Journal of Science and Technology (WJST), 16(11), 867–873. https://doi.org/10.48048/wjst.2019.4391

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Research Article