Production and Properties of Biosurfactant from Marine Isolated Pectinatus cerevisiiphilus CT3

chanika saenge chooklin

Abstract


        A marine bacterial isolated, Pectinatus cerevisiiphilus CT3, was able to grow and produce biosurfactant on minimal salts medium using glucose and NaNO3 as a carbon and nitrogen sources. It was found that cellular growth and biosurfactant production in MSM were greatly affected by the medium components. After 54 h of cultivation, P. cerevisiiphilus CT3 was able to grow and produce surfactant reducing the surface tension of medium to 28.0 mN/m and giving biosurfactant concentration of 3.05 g/l with the critical micelle concentrations of 10 mg/l, respectively. The biosurfactant recovery by chloroform: methanol extraction showed pH and thermal stability with respect to surface tension reduction. It also showed emulsification activity and a high level of salt concentration. Furthermore, it revealed a promising antimicrobial activity against tested human pathogenic bacterial and fungal isolates. Based on these results, the isolated biosurfactant from the marine P. cerevisiiphilus CT3 revealed board physicochemical stabilities and possess excellent antimicrobial activities which proves its significance for possible use in various therapeutic and biomedical applications. 


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