In vitro Shoot Cultures of Tupistra albiflora K. Larsen

Jiraporn PALEE

doi:10.48048/wjst.2020.4182

Authors

Jiraporn PALEE Science Program, Faculty of Education, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.4182

Keywords:

Tupistra albiflora, micropropagation, in vitro shoot culture, BA, NAA

Abstract

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

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