A Low Cost and Simple Lab-on-a-Chip for Spectrophotometric Determination of Ethanol



A simple lab-on-a-chip system was developed for the rapid determination of ethanol in different sample matrices, including gasohol and various alcoholic beverages. The colorimetric detection of ethanol using a spectrophotometer was based on the reaction between ethanol with 0.12 M ceric ammonium nitrate in acidic medium to produce a red colored product which gave a maximum absorption at 470 nm. A non-lithographic method was used for creating lab-on-a-chip molds to reduce  manufacturing cost and preparation steps. The lab-on-a-chip device was fabricated from polydimethylsiloxane which consisted of a simple Y-shaped working channel. Under optimum conditions, a linear calibration graph was obtained in the concentration range of 0.20 - 20 % (v/v) (r2> 0.999). The limit of detection (3 SD) and limit of quantification (10 SD) were 0.039 and 0.13 % (v/v), respectively. The precision reported in terms of relative standard deviation (RSD) values was less than 1.40 % (n = 15). To demonstrate the lab-on-a-chip’s performance, the determination of ethanol in gasohol and various alcoholic beverages was applied. The results obtained from the developed method compared with a standard gas chromatographic method were well correlated using the paired t-test and linear regression test. The results indicate that the proposed method has shown potential to extend the use of this simple lab-on-a-chip analytical device, due to its simplicity, low cost, lower reagent and sample consumption and high analytical performance. Moreover, the method of fabrication would be an additive manufacturing technique featuring a low equipment cost with no need for clean rooms.


Lab-on-a-chip, ethanol, alcoholic beverage, gasohol

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Import Alcohol to Thailand. Available at: http://www.interactivethailand.com/corporate/business-licenses/ import-alcohol-to-thailand, accessed February 2017.

Alcoholic Drink. Available at: https://en.wikipedia.org/wiki/Alcoholic_drink, accessed February 2017.

C Bloyd. An Update on Ethanol Production and Utilization in Thailand. Available at: http://www.pnl.gov/main/publications/external/technical_reports/PNNL-19060.pdf, accessed February 2017.

Common Ethanol Fuel Mixtures, Available at: https://en.wikipedia.org/wiki/Common_ethanol_ fuel_mixtures, accessed February 2017.

GK Buckee and AP Mundy. Determination of ethanol in beer by gas chromatography (Direct-Injection)-Collaborative trial. J. Inst. Brew. 1993; 99, 381-4.

H Lia, XS Chaia, Y Dengc, H Zhana and S Fua. Rapid determination of ethanol in fermentation liquor by full evaporation head-space gas chromatography. J. Chromatogr. A 2009; 1216, 169-72.

D Lefebvre, V Gabriel, Y Vayssier and C Fontagné-Faucher. Simultaneous HPLC determination of sugars, organic acids and ethanol in sourdough process. Lebensm. Wiss. Tech. 2002; 35, 407-14.

E Akyilmaz and E Dinçkaya. An amperometric microbial biosensor development based on Candida tropicalis yeast cells for sensitive determination of ethanol. Biosens. Bioelectron. 2005; 20, 1263-9.

AM Pisoschi, A Popa, AI Serbana and GP Negulescuc. Ethanol determination by an Amperometric bienzyme sensor based on a clark-type transducer. J. Electroanal. Chem. 2012; 671, 85-91.

L Rotariu, C Bala and V Magearu. New potentiometric microbial biosensor for ethanol determination in alcoholic beverages. Anal. Chim. Acta 2004; 513, 119-23.

JJ Kabara and J Smeggil. A colorimetric method of determining the presence of ethanol in the blood by the use of Ceric Ammonium nitrate as a color reagent. J. Am. Osteopath. Assoc. 1973; 72, 65-70.

S Vicente, EAG Zagatto, PCAG Pinto, MLMES Saraiva, JLFC Lima and EP Borges. Exploiting gas diffusion for non-invasive sampling in flow analysis: Determination of ethanol in alcoholic beverages. J. Phys. Chem. Lett. 2006; 78, 23-9.

N Choengchan, T Mantim, P Wilairat, PK Dasgupta, S Motomizu and D Nacapricha. A membraneless gas diffusion unit: Design and its application to determination of ethanol in liquors by spectrophotometric flow injection. Anal. Chim. Acta 2006; 579, 33-7.

S Muncharoen, J Sitanurak, W Tiyapongpattana, N Choengchan, N Ratanawimarnwong, S Motomizu, P Wilairat and D Nacapricha. Quality control of gasohol using a micro-unit for membraneless gas diffusion. Microchim. Acta 2009; 164, 203-10.

P Pinyou, N Youngvises and J Jakmunee. Flow injection colorimetric method using acidic ceric nitrate as reagent for determination of ethanol. Talanta 2011; 84, 745-51.

CR Silva, TF Gomes, VA Barros and EA Zagatto. A multi-purpose flow manifold for the spectrophotometric determination of sulphide, sulphite and ethanol involving gas diffusion: Application to wine and molasses analysis. Talanta 2013; 113, 118-22.

I Šrámková, B Horstkotte, P Solich and H Sklenářová. Automated in-syringe single-drop head-space micro-extraction applied to the determination of ethanol in wine samples. Anal. Chim. Acta 2014; 828, 53-60.

Advantages of LOCs. Available at: https://en.wikipedia.org/wiki/Lab-on-a-chip, accessed March 2017.

YC Lim, AZ Kouzani and W Duan. Lab-on-a-chip: A component view. Microsyst. Tech. 2010; 16, 1995-2015.

H Yu, G Zhou, FS Chau, S Wang and F Lee. Novel polydimethylsiloxane (PDMS) based microchannel fabrication method for lab-on-a-chip application. Sensors Actuat. B Chem. 2009; 137, 754-61.

Y Temiz, RD Lovchik, GV Kaigala and E Delamarche. Lab-on-a-chip devices: How to close and plug the lab? Microelectron. Eng. 2015; 132, 156-75.

L Lei, IL Mattos and Y Chen. Microfluidic devices for optical determination of ethanol concentration. Microelectron. Eng. 2008; 85, 1318-20.


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Last updated: 17 May 2019