Radical scavenging and anti-inflammatory properties of pectin from Cissampelos pareira Linn.

Nakuntwalai WISIDSRI, Suradwadee THUNGMUNGMEE


Cissampelos pareira Linn. (C. pareira) has been used as a medicinal herb for treating fever and analgesic by Indian and Thai people. This experimental research investigates the scavenging ability of C. pareira pectin from leaves of variable concentrations on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) free radicals; and its anti-inflammatory property in the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells and the cell viability. The experimental results show that the DPPH and NO scavenging performances of C. pareira pectin are positively correlated to the pectin concentrations, with the corresponding half maximal inhibitory concentrations (IC50)of 0.54 and 0.52 mg/ml. Meanwhile, the NO production in the LPS-stimulated macrophage cells is inversely correlated to the pectin concentrations. The cell viability in the LPS-stimulated macrophage cells is positively correlated to the C. pareira pectin concentrations, given the non-cytotoxicity of the extract compound. In essence, the inhibition of free radicals and suppression of activated macrophages point to the usefulness of C. pareira pectin in functional dietary products and herb-based pharmaceuticals.


Cissampelos pareira Linn., pectin, radical scavenging, anti-inflammation

Full Text:



E Birben, UM Sahiner, C Sackesen, S Erzurum, O Kalayci, Oxidative stress and antioxidant defense. World Allergy Organ J. 2012; 5 (1), 9-19.

VI Lushchak. Free radicals, reactive oxygen species, oxidative stress and its classification. Chem-Biol Interact. 2014; 224, 164-175.

SK Choudhari, M Chaudhary, S Bagde, AR Gadbail, V Joshi. Nitric oxide and cancer: a review. World J Surg Oncol. 2013; 11, 118.

M Valko, D Leibfritz, J Moncol, MT Cronin, M Mazur. J Telser, Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007; 39, 44-84.

LA Pham-Huy, H He, C Pham-Huy. Free radicals, antioxidants in disease and health. Int J Biomed Sci. 2008; 4, 89-96.

N Khansari, Y Shakiba, M Mahmoudi. Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent Pat Inflamm Allergy Drug Discov. 2009; 3, 73-80.

HT Lu, WM Ouyang, CS Huang, Inflammation, a key event in cancer development. Mol Cancer Res. 2006; 4, 221-233.

H. Bartsch and J. Nair. Chronic inflammation and oxidative stress in the genesis and perpetuation of cancer: role of lipid peroxidation, DNA damage, and repair. Langenbecks Arch Surg. 2006; 391, 499-510.

G Amresh, CV Rao, PN Singh, Antioxidant activity of Cissampelos pareira on benzo(a)pyrene-induced mucosal injury in mice. Nutr Res. 2007; 27, 625-632.

A Bafna, S Mishra, Antioxidant and immunomodulatory activity of the alkaloidal fraction of Cissampelos pareira linn. Sci Pharm. 2010; 78, 21-31.

G Amresh, GD Reddy, V Rao Ch, PN Singh. Evaluation of anti-inflammatory activity of Cissampelos pareira root in rats. J Ethnopharmacol. 2007; 110, 526-531.

G Amresh, PN Singh, V Rao Ch. Antinociceptive and antiarthritic activity of Cissampelos pareira roots. J Ethnopharmacol. 2007; 111, 531-536.

HM Reza, M Shohel, SB Aziz, FI Pinaz, MF Uddin, M Al-Amin, IN Khan, P Jain. Phytochemical and pharmacological investigation of ethanol extract of Cissampelos pareira. Indian J Pharm Sci. 2014; 76, 455-458.

BS Thavamani, M Mathew, SP Dhanabal. Anticancer activity of cissampelos pareira against dalton's lymphoma ascites bearing mice. Pharmacogn Mag. 2014; 10, 200-206.

A Arkarapanthu, V Chavasit, P Sungpuag, L Phuphathanaphong. Gel extracted from Khruea-ma-noi (Cyclea barbata Miers) leaves: chemical composition and gelation properties. J Sci Food Agr. 2005; 85, 1741-1749.

J Singthong, S Ningsanond, SW Cui, HD Goff. Extraction and physicochemical characterization of Krueo Ma Noy pectin. Food Hydrocolloid. 2005; 19, 793-801.

J Singthong, SW Cui, S Ningsanond, HD Goff. Structural characterization, degree of esterification and some gelling properties of Krueo Ma Noy (Cissampelos pareira) pectin. Carbohyd Polym. 2004; 58, 391-400.

Y Sumanont, Y Murakami, M Tohda, O Vajragupta, K Matsumoto, H Watanabe. Evaluation of the nitric oxide radical scavenging activity of manganese complexes of curcumin and its derivative. Biol Pharm Bull. 2004; 27, 170-173.

J Kubola and S Siriamornpun. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chem. 2008; 110, 881-890.

V Urias-Orona, J Huerta-Oros, E Carvajal-Millan, J Lizardi-Mendoza, A Rascon-Chu, AA Gardea. Component analysis and free radicals scavenging activity of Cicer arietinum L. Husk Pectin. Molecules. 2010; 15, 6948-6955.

V Hawach, MA Boujaoude, RM Abdel-Massih. The cytotoxic and anti-proliferative activity of high molecular weight pectin and modified citrus pectin. Funct Foods Health D. 2016; 6, 587-601.

O Rop, T Jurikova, J Sochor, J Mlcek, D Kramarova, Antioxidant capacity, scavenging radical activity and selected chemical composition of native apple cultivars from central Europe. J Food Quality. 2011; 34, 187-194.

P Arulselvan, MT Fard, WS Tan, S Gothai, S Fakurazi, ME Norhaizan, SS Kumar. Role of antioxidants and natural products in inflammation. Oxid Med Cell Longev. 2016, DOI: 10.1155/2016/5276130

V Lobo, A Patil, A Phatak, N Chandra. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4, 118-126.

HX Chen, M Zhang, BJ Xie. Quantification of uronic acids in tea polysaccharides conjugates and its antioxidant properties. J. Agric. Food Chem. 2004; 52, 3333-3336.

A Rahal, A Kumar, V Singh, B Yadav, R Tiwari, S Chakraborty, K Dhama. Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res Int. 2014, DOI: 10.1155/2014/761264.

SK Biswas. Does the interdependence between oxidative stress and inflammation explain the antioxidant paradox?. Oxid Med Cell Longev. 2016, DOI: 10.1155/2016/5698931.

CH Chen, MT Sheu, TF Chen, YC Wang, WC Hou, DZ Liu, TC Chung, YC Liang. Suppression of endotoxin-induced proinflammatory responses by citrus pectin through blocking LPS signaling pathways. Biochem Pharmacol. 2006; 72, 1001-1009.

S Popov, P Markov, G Popov, I Nikitin, L Efimov, Y Ovodov. Anti-inflammatory activity of low and high methoxylated citrus pectins. Biomedicine & Preventive Nutrition. 2013; 3, 59–63.

SV Popov, RG Ovodova, VV Golovchenko, DS Khramova, PA Markov, VV Smirnov, AS Shashkov, YS Ovodov. Pectic polysaccharides of the fresh plum Prunus domestica L. isolated with a simulated gastric fluid and their anti-inflammatory and antioxidant activities. Food Chem. 2014; 143, 106-113.

PA Markov, SV Popov, IR Nikitina, RG Ovodova, YS Ovodov. Anti-inflammatory activity of pectins and their galacturonan backbone. Russ J Bioorg Chem. 2011; 37, 817-821.

J Muller-Maatsch, M Bencivenni, A Caligiani, T Tedeschi, G Bruggeman, M Bosch, J Petrusan, B Van Droogenbroeck, K Elst, S Sforza. Pectin content and composition from different food waste streams. Food Chemistry. 2016; 201, 37-45.

F Naqash, FA Masoodi, SA Rather, SM Wani, A Gani. Emerging concepts in the nutraceutical and functional properties of pectin-A Review. Carbohydr Polym. 2017; 168, 227-239.

MA Poiana, MFMunteanu, DM Bordean, R Gligor, E Alexa. Assessing the effects of different pectins addition on color quality and antioxidant properties of blackberry jam. Chem Cent J. 2013; 7, 121.

F Munarin, MC Tanzi, P Petrini. Advances in biomedical applications of pectin gels. Int J Biol Macromol. 2013; 55, 307-307.


  • There are currently no refbacks.


Online ISSN: 2228-835X


Last updated: 20 June 2019