Total Flavonoids, Total Phenolic Content and Antioxidant Activity from Fruits, Leaves, Twigs and Flowers of Mesua ferrea L.

Prakit CHAITHADA; Juthamas SUPAPAN; Juthamas SUPAPAN; Phetcharat RODTHUK; Phetcharat RODTHUK; Saowalak CHAINARONG; Saowalak CHAINARONG

doi:10.48048/wjst.2018.3946

Authors

Prakit CHAITHADA Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280
Juthamas SUPAPAN Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280
Juthamas SUPAPAN Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280
Phetcharat RODTHUK Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280
Phetcharat RODTHUK Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280
Saowalak CHAINARONG Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280
Saowalak CHAINARONG Department of General Science, Faculty of Education, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280

DOI:

https://doi.org/10.48048/wjst.2018.3946

Keywords:

Total flavonoids content, total phenolic content, antioxidants, Mesua ferrea L.

Abstract

The objective of this study was to compare antioxidant activity from crudes of fruits, leaves, twigs and flowers of Mesua ferrea L. with hexane, dichloromethane and methanol by controlling time and temperature in the solvent extraction. All crude extracts were investigated for their antioxidant capacity in 1,1-diphenenyl-2-picryhydrazyl (DPPH) radical scavenging. The results indicated that the high polar solvent exhibited the highest DPPH radical scavenging activity. The methanol extracts of leaves show the highest activity with IC₅₀ of 89.94±0.65 μg/mL. The flowers, twigs and fruits methanol crude extracts show IC₅₀ of 94.26±5.93, 94.45±1.67 and 722.94±5.85 μg/mL, respectively. The methanol extracts of leaves also had the highest total flavonoids content (325.79±3.08mgQE/g). The methanol extract of flowers and the dichloromethane extract of fruits presented higher total phenolic contents than the other extracts which contained 769.11±46.64 mgGAE/g and 703.62±12.62 mgGAE/g, respectively, followed by the methanol extract of leaves (348.36±38.53 mgGAE/g).

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

ST Ho, YT Tung, KC Cheng and JH Wu. Screening, determination and quantification of major antioxidants from Balanophora laxiflora flowers. Food Chem. 2010; 122, 584-8.

J Fan, H Feng, Y Yu, M Sun, Y Liu, T Li, X Sun, S Liu and M Sun. Antioxidant activities of the polysaccharides of Chuanminshen violaceum. Carbohydr. Polym. 2017; 157, 629-36.

KA Steinmetz and JD Potter. Vegetables, fruit, and cancer prevention: A review. J. Am. Diet Assoc. 1996; 96, 1027-39.

J Parr and GP Bolwell. Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile. J. Sci. Food Agr. 2000; 80, 985-1012.

P Roopesh, AK Nayanatara, K Reshma and S Ganesh. Evaluation of in vitro antioxidant properties of hydro alcoholic extract of entire plant of Cynodon dactylon. J. Young Pharm. 2016; 8, 378-84.

S Akhila, AR Bindu, K Bindu and NA Aleykutty. Comparative evaluation of extracts of Citrus limon burm peel for antioxidant activity. J. Young Pharm. 2009; 1, 136-40.

MK Chahar, DSS Kumar, T Lokesh and KP Manohara. In vivo antioxidant and immunomodulatory activity of mesuol isolated from Mesua ferrea L. seed oil. Int. Immunopharm. 2012; 13, 386-91.

KP Rajesh, H Manjunatha, V Krishna and SBE Kumara. Potential in vitro antioxidant and protective effects of Mesua ferrea Linn. bark extracts on induced oxidative damage. Ind. Crop. Prod. 2013; 47, 186-98.

K Awang, G Chan, M Litaudon, NH Ismail, MT Martin and F Gueritte. 4-Phenylcoumarins from Mesua elegans with acetylcholinesterase inhibitory activity. Bioorgan. Med. Chem. 2010; 18, 7873-7.

B Tepe, M Sokmen, HA Akpulat and A Sokmen. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem. 2006; 95, 200-4.

SD Ramaiya, JS Bujang and MH Zakaria. Assessment of total phenolic, antioxidant, and antibacterial activities of Passiflora species. Sci. World J. 2014; 2014, 167309.

A Rebaya, SI Belghith, B Baghdikian VM Leddet, F Mabrouki, E Olivier, JK Cherif and MT Ayadi. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). J. Appl. Pharmaceut. Sci. 2015; 5, 52-7.

A Crozier and H Ashihara. Plant Secondary Metabolites and the Human Diet. Blackwell Publishing, Oxford, 2006.

KE Arash, MT Rosna, M Sadegh and B Behrooz. Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pretense L. (red clover). Biomed. Res. Int. 2015; 2015, 643285.

AR Fuad, R Suzi, HA Sharehan, FK Mahmoud, W Ismail and S Zaidoun. Anticancer activity, antioxidant activity, and phenolic and flavonoids content of wild Tragopogon porrifolius plant extracts. Evid. Base. Compl. Alternat. Med. 2016; 2016, 9612490.