Utilization of Paper-Cone Water Cups as an Alternative Lignocellulose Waste Substrate in Pleurotus ostreatus Production

Suvit SUWANNO, Aminoh AYAE, Nuttida SUWANNO

Abstract


This study examined the utilization of paper-cone water cups as an alternative substrate for oyster mushroom (Pleurotus ostreatus) production. The research was conducted by using bioconversion technology and a profitable method for converting lignocellulosic residue from municipal solid waste into protein-rich biomass, thereby reducing waste paper and enhancing environmental quality. The most suitable substrate for mycelial growth was waste paper from paper-cone water cups (WPC) combined with rubber wood sawdust (RWS) at a 75%:25% dry basis ratio. The substrate mixture was adjusted to a moisture content of 70 %, and the C/N ratio was fixed at 20:1 by the addition of urea and supplementation with 8 % rice bran. Spawn running used 10 % seed inoculum. The mushrooms were cultivated on 500 g of substrate in polyvinyl chloride boxes (405 cm3) and incubated at 25 °C in the dark with the relative humidity maintained at 70 - 80 %. The fastest spawn running (mycelia development) occurred at 5th days, with pin head formation at 9th days and fruiting body formation at 12th days.The highest yield recorded was 26.59g/100g.Under these conditions, the potential lignocellulosic waste conversion (biological efficiency) was recorded as 88.64 %, and the protein content of P. ostreatus was 35.75 % after 12 days of cultivation.


Keywords


Organic waste utilization, Lignocellulosic waste, Bioconversion, Pleurotus ostreatus, Environmental management

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References


Packaging Intelligence Unit.Thailand Packaging Industry Market Reports.Available at: http://packaging.oie.go.th/new/search_manufacturing_data.php?ACTION=SEARCH, accessed May 2018.

A Sharp and J Sang-Arun. A Guide for Sustainable Urban Organic Waste Management in Thailand: Combining Food, Energy, and Climate Co-benefits. IGES Policy report. Asia-Pacific Network for Global Change Research (APN) Thailand, 2012, p. 17-43.

A Challcharoenwattana and C Pharino. Co-benefits of household waste recycling for local community’s sustainable waste management in Thailand. Sustainability 2015; 7, 7417-37.

C Chiemchairi, JP Juanga and C Visvanathan. Municipal solid waste management in Thailand and disposal emission inventory. Environ. Monit. Assess. 2007; 135, 13-20.

M Badu, SK Twumasi and NO Boadi. Effect of lignocellulosic in wood used as substrate on the quality yield of mushroom. Food Nutr. Sci. 2011; 2, 780-4.

T Chinda, N Leewattana and N Leeamnuayjaroen. The study of Landill sitable in Thailand. In: Proceedings of the 1st Mae Fah Lunng University International Conference, Chiang Rai, Thailand, 2012, p.1-8.

S Yildiz, ÜC Yildiz, ED Gezer and A Temiz. Some lignocellulosic wastes uses as raw material in cultivation of the Pleurotus ostreatus culture mushroom. Process. Biochem. 2002; 38, 301-6.

VP Mane, SS Patil, AA Syed and MMV Baig. Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju (Fr.) Singer. J. Zhejiang Univ. Sci. B 2007; 8, 745-51.

EA Adebayo and D Martinez-Carrera. Oyster mushrooms (Pleuurotus) are useful for utilizing lignocellulosic biomass. Afr. J. Biotech. 2015; 14, 52-67.

R Naraian, MP Singh and S Ram. Supplementation of basal substrate to boost up substrate strength and Oyster mushroom yield: An overview of substrates and supplements. Int. J. Curr. Microbiol. Appl. Sci. 2016; 5, 543-53.

R Zhang, X Li and JG Fadel. Oyster mushroom cultivation with rice and wheat straw. Bioresour. Tech. 2012; 82, 277-84.

M Dashtban, H Schrft and W Qin. Fungal bioconversion of lignocellulosic residues: Opportunities & perspectives. Int. J. Biol. Sci. 2009; 5, 578-95.

DJ Royse, J Baarsand and Q Tan. Current Overview of Mushroom Production in the World. In: DC Zied and A Pardo-Gimenez (eds.). Edible and Medicinal Mushrooms: Technology and Applications. Wiley, New York, 2017, p. 5-12.

LJLD Van-Griensven. Mushrooms as a Conventional Food Mushrooms: Conventional Food and Alternative Medicine. In: I Milenkovic and I MIlosavljevic (eds.). Mushroom Cultivation Manual for the Small Mushroom Entrepreneur. Eko-offungi, Belgrade, Serbia, 2017, p. 7-16.

JK Oloke and EA Adebayo. Effectveness of immunotherapies from oyster mushroom (Pleurotus species) in the management of immunocompromised patients. Int. J. Immunol. 2015; 3, 8-20.

A Gregori, M Svagelj and J Pohleve. Cultivation techniques and medicinal properties of Pleurotus spp. Food Tech. Biotech. 2007; 45, 238-49.

RP Tengerdy and G Szakacs. Bioconversion of lignocellulose in solid substrate fermentation. Biochem. Eng. J. 2003; 13, 169-79.

ST Chang and SP Wasser. The Cultivation and Environmental Impact of Mushrooms. Oxford Research encyclopedia of environmental science. Oxford University Press, Oxford, 2017.

AN Philippoussis, G Zervakis and P Diamantopoulou. Bioconversion of agricultural lignocllulosic waste through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World J. Microbiol. Biotech. 2001; 17, 191-200.

Q Mandeel, AAA Al-Laith and SA Mohamed. Cultivation on oyster mushroom (Pleurotus spp.) on various lignocellulosic wastes. World J. Microbiol. Biotech. 2005; 21, 601-7.

G Koutrotsios, KC Mountzouris, I Chatzipavlidis and GI Zervakis. Bioconversion of lignocellulosic residues by Agrocybe cylindracea and Pleurotus ostreatus mushroom fungi: Assessment of their effect on the final product and spent substrate properties. Food Chem. 2014; 61, 127-35.

R Sindnu, P Binod and A Pandey. Biological pretreatment of lignocellulosic biomass: An overview. Bioresour. Tech. 2016; 199, 76-82.

S Papong, C Yuvaniyama, P Lohsomboon and P Malakul. Overview of biomass utilization in Thailand. In: Proceedings of the Meeting for LCA in ASEAN Biomass Project, International Conference Center Bangkok, 2004.

S Yildiz, ÜC Yildiz, ED Gezer and A Temiz. Some lignocellulosic wastes uses as raw material in cultivation of the Pleurotus ostreatus culture mushroom. Process. Biochem. 202; 38, 301-6.

R Zhang, X Li and JG Fadel. Oyster mushroom cultivation with rice and wheat straw. Bioresour. Tech. 2002; 82, 277-84.

E Harada, T Morizono, T Sumiya and S Meguro. Production of andean-patagonic edible mushroom Grifola gargal on wood-based substrates. Mycoscience 2015; 56, 616-21.

E Baysal, H Peker, MM Yalinkiliҫ and A Temiz. Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresour. Tech. 2003; 89, 95-7.

Association of Official Analytical Chemists (AOAC). Official Methods of Analysis of the Association of Official Analytical Chemists. 19th ed. AOAC lnc. Washington DC, USA, 2012.

R Novozamsky, JV Eck, CH Shcouwenburg and VI Wallinga. Total nitrogen determination in plant material by means of the indophenol blue method. J. Agri. Sci. 1974; 22, 3-5.

A Walkley and IA Black. An examination of degtiareff method for determining soil organic matter and apropos modification of the chromic acid titration method. Soil Sci. 1934; 37, 29-38.

G Tchobanoglous, H Thesisen and S Vigil. Integrated Solid Waste Management Engineering Principles and Management Issues. McGraw-Hill, Singapore, 1993.

KW Choi. Shelf Cultivation of Oster Mushroom. Mushroom Growers Handbook 1 Oyster Cultivation. Mush World, 2004, p. 153-65.

PA Philippoussis, PA Diamantopoulou and GI Zervakis. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes. World J. Microbiol. Biotech. 2003; 19, 551-7.

D Salmones, M Gerardo, LM Raos and KN Waliszewski. Cultivation of Shitatake mushroom, Lentimula edodes in several lignocellulosic materials originating from the tropics. Agronomie 1999; 19, 13-9.

M Obodia, J Cleland-Okine and KA Vowotor. Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. J. Ind. Microbiol. Biot. 2003; 30, 146-9.

EA Adebayo and D Martiinez-Carrera. Oster mushroom (Pleurotus) are useful for utilizing lignocellulosic biomass. Afr. J. Biotech. 2015; 14, 52-67.

Z Junior, L Luiz, GA Linde and NB Colauto. Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method. Acta Sci. Agron. 2010; 32, 55-60.

S Rezaean and H Pourianfar. A comparative study on bioconversion of different agro wastes by wild and cultivated strains of Flammulina velutipes. Waste Biomass Valori. 2016; 8, 2631-42.

M Moonmoon, MN Uddin, S Ahmed, NJ Shelly and Md A Khan. Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on sawdust and rice straw in Bangladesh. Saudi J. Biol. Sci. 2010; 17, 341-5.

A Tesfaw, A Tadesse and G Kiros. Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia. Appl. Microbiol. Biotech. 2015; 3, 15-20.

R Naraian, RK Sahu, S Kumar, SK Garg, CS Singh and RS Kanaujia. Influence of different nitrogen rich supplements during cultivation of Pleurotus florida on corncob substrate. Environmentalist 2009; 29, 1-7.

M Mansour-Benamar, JM Savoie and L Chavant. Valorization of solid olive mill wastes by cultivation of a local strain of edible mushrooms. Comptes Rendus Biol. 2013; 336, 407-15.

MI Bhatti, MM Jiskani, KH Wagan, MA Pathan and MR Magsi. Growth, development and yield of oyster mushroom, Pleuotus ostreatus (JACQ. EX. FR.) Kummer as affected by different spawn rates. Pak. J. Bot. 2007; 39, 2685-92.

S Sharma, RPK Yadav and CP Pokhrel. Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. J. New Biol. Rep. 2013; 2, 3-8.


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