Comparative Antioxidant Activity and Volatile Oil Composition of Leaves and Fruits of Thuja orientalis Growing in Egypt

Abeer MOAWAD; Elham AMIN

doi:10.48048/wjst.2019.3269

Authors

Abeer MOAWAD Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514
Elham AMIN Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514

DOI:

https://doi.org/10.48048/wjst.2019.3269

Keywords:

Thuja orientalis, volatile oil, total phenolic, total flavonoids, DPPH

Abstract

Thuja orientalis L. (Cupressaceae) is an evergreen arbor that is distributed throughout Northeast Asia as a common ornamental plant. The volatile oil of leaves and fruits of T. orientalis growing in Egypt was prepared by hydro-distillation followed by GC-MS analysis in order to compare between their compositions. Results revealed that fruits are richer in monoterpenes (62.5 %) while leaves are richer in sesquiterpenes (57 %). The major components in leaf oil are; α-cedrol (15.8 %), β-caryophyllene (15 %), α-humulene (10.7 %), d-limonene (7.3 %), α-pinene (6.9 %), β-myrecene (5.9 %) and α-terpinolene (5.2 %). On the other hand, fruit oil contains α-pinene (11.3 %), α-cedrol (11.2 %), β-myrecene (9.6 %), geranyl acetate (9 %) and β-caryophyllene (8.9 %) in major amounts. Comparison between the oil content in the Egyptian conifer and other reported conifers worldwide is also discussed. Comparative determination of total phenolic content (TPC), total flavonoids content (TFC) and anti-oxidant activity of alcoholic extract of both leaves and fruits showed that fruits content of flavonoids (0.6267±0.026 mg RE /g) is double the content of the leaves (0.3069±0.019 mg RE /g). Similarly, fruits contain higher phenolic content (0.75±0.007 mg GE/g) compared to 0.51±0.007 mg GE/g in leaves. Also, fruits extract exhibited higher anti-oxidant activity (2.3516±0.0 mg AEAC/g) than leaves (1.2734±0.0022 mg AEAC/g). The extracts of T. orientalis could be a valuable material for pharmaceutical industry.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

TH Kim, H Li, Q Wu, HJ Lee and JH Ryu. A new labdane diterpenoid with anti-inflammatory activity from Thuja orientalis. J. Ethnopharmacol. 2013; 146, 760-7.

N Zhang, DK Park and HJ Park. Hair growth-promoting activity of hot water extract of Thuja orientalis. BMC Complement. Altern. Med. 2013; 13, 9.

EH Lee, DG Song, JY Lee, CH Pan, BH Um and SH Jung. Flavonoids from the leaves of Thuja orientalis inhibit the aldose reductase and the formation of advanced glycation endproducts. J. Korean Soc. Appl. Biol. Chem. 2009; 52, 448-55.

S Emami, J Asili, Z Mohagheghi and MK Hassanzadeh. Antioxidant activity of leaves and fruits of Iranian conifers. Evid. Based Complement. Alternat. Med. 2007; 4, 313-9.

A Singh and VK Singh. Molluscicidal activity of Saraca asoca and Thuja orientalis against the fresh water snail Lymnaea acuminata. Vet. Parasitol. 2009; 164, 206-10.

H Lei, Y Wang, F Liang, W Su, Y Feng and X Guo. Composition and variability of essential oils of Platycladus orientalis growing in China. Biochem. Syst. Ecol. 2010; 38, 1000-6.

YD Chen, SX Li, L Yang, ZY Jiang and NH Cui. Comparative study on chemical constituents of essential oils from several parts of Platycladus orientlais. Chem. Indust. Forest 1984; 4, 1-11.

TL Liu, Q Qiu, Y Zhao, YM Shen and J Wang. Study on chemical constituents of essential oil of Biota orientlais by GC-MS. J. Chin. Med. Mater. 2000; 23, 460-1.

RH Hui, DY Hou, XY Liu, XC Li and HL Geng. Analysis of volatile components from leaf twigs in Biota orientalis with different extraction methods by gas chromatography-mass spectrometry. J. Chin. Mass Spectrom. Soc. 2006; 27, 226-31.

H Lei, Y Wang, F Liang, W Su, Y Feng, X Guo and N Wang. Composition and variability of essential oils of Platycladus orientalis growing in China. Biochem. Syst. Ecol. 2010; 3, 1000-6.

MK Hassanzadeh, M Rahimizadeh, BS Fazly Bazzaz, SA Emami and J Assili, Chemical and antimicrobial studies of Platycladus orientalis essential oils. Pharm. Biol. 2001; 39, 388-90.

B Nickavar, G Amin and S Parhami. Volatile constituents of the fruit and leaf oils of Thuja orientalis L . grown in Iran. Z. Naturforsch. 2003; 58, 171-2.

S Afsharypuor and B Nayebzadeh. Essential oil constituents of young stem, leaf and fruit of Platycladus orientalis (L.) Franco grown in Isfahan (Iran). J. Essent. Oil Res. 2009; 21, 525-28.

DN Dai, TD Thang and IA Ogunwande. Terpene composition of three species of Gymnosperms from Vietnam. Am. J. Plant Sci. 2013; 4, 2031-8.

M Riaz, MK Rashid and FM Chaudary. Volatile constituents of the leaves Pakistani Cupressus sempervirens and Thuja orientalis. Pak. J. Sci. Indust. Res. 1999; 42, 98-101.

M Riaz, S Qamar, M K Rashid and FM Chaudary. Chemical composition of Thuja orientalis L. fruits at different stages of maturity. Pak. J. Sci. Indust. Res. 1999; 42, 188-91.

R Chizzola, W Hochsteiner and S Hajek. GC Analysis of essential oils in the rumen fluid after incubation of Thuja orientalis twigs in the rusitec system. Res. Vet. Sci. 2004; 76, 77-82.

IA Ogunwande, NO Olawore, KA Adeleke and O Ekundayo. Composition of the volatile oil of Thuja ori entalis L. from Nigeria. J. Trop. Forest Resour. 2003; 19, 41-7.

S Guleria, A Kumar and AK Tiku. Chemical composition and fungitoxic activity of essential oil of Thuja orientalis L. grown in the north-western Himalaya. Z. Naturforsch. 2008; 63, 211-4.

A An, M Sunanda, B Shubhangi, A Jain and M Manisha. Pharmacognostic, phytochemical, and pharmacological investigation on bark of Thuja orientalis Linn (Cupressaceae). J. Pharmacogn. Phytochem. 2016; 5, 111-3.

ND Jasuja1, SK Sharma, R Saxena, J Choudhary, R Sharma and SC Joshi. Antibacterial, antioxidant and phytochemical investigation of Thuja orientalis leaves. J. Med. Plants. Res. 2013; 7, 1886-93.

WA Shah and M Qadir. Chemical composition, antioxidant and antibacterial activity of Thuja orientalis essential oil. World J. Pharm. Sci. 2014; 2, 56-61.

G Kayaa, R Karakaya, E Tilgel, M Sandikçı, E Yücel, G Çicek, Ö Kayır, H Akşit, İ Telci, A Güzel, M Elmastas and R Erenler. Essential oil constituents of Thuja orientalis berries. J. New Results Sci. 2014; 7, 1-6.

I Amri, M Hanana, B Jamoussi and L Hamrouni. Chemical composition of Thuja orientalis L. essential oils and study of their allelopathic potential on germination and seedling growth of weeds. Arch. Phytopath. Plant Protect. 2014; 48, 18-27.

MJ Khubeiz, G Mansour and B Zahraa. Antibacterial and phytochemical investigation of Thuja orientalis (L.) leaves essential oil from Syria. Int. J. Curr. Pharmaceut. Rev. Res. 2016; 7, 243-7.

MF Abu Bakar, M Mohamed, A Rahmat and J Fry. Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chem. 2009; 113, 479-83.

LM Magalhães, M Segundo, S Reis and JL Lima. Automatic method for determination of total antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl assay. Anal. Chim. Acta 2006; 558, 310-8.

ER Elsharkawy, H Aljohar and AM Donia. Comparative study of antioxidant and anticancer activity of Thuja orientalis growing in Egypt and Saudi Arabia. Br. J. Pharmaceut. Res. 2017; 15, 1-9.

Xu GH, IJ Ryoo, YH Kim, SJ Choo and ID Yoo. Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. Arch. Pharm. Res. 2009; 32, 275-82.