Callus Induction and Somatic Embryogenesis from Cultured Zygotic Embryo of Eleiodoxa conferta (Griff.) Burr., an Edible Native Plant Species in Southern Thailand

Duangkhaetita KANJANASOPA; Benjamas SOMWONG; Theera SRISAWAT; Suraphon THITITHANAKUL; Yoawaphan SONTIKUL; Suchart CHOENGTHONG

Authors

Duangkhaetita KANJANASOPA Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000
Benjamas SOMWONG Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000
Theera SRISAWAT Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000
Suraphon THITITHANAKUL Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000
Yoawaphan SONTIKUL Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000
Suchart CHOENGTHONG Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000

Keywords:

Callus, embryo, in vitro propagation, tissue culture, Eleiodoxa conferta (Griff.) Burr.

Abstract

This research aimed to study the in vitro culturing of Eleiodoxa conferta (Griff.) Burr., collected from the Natural Study Center of Khan Thuli Peat Swamp Forest, located at Amphor Tha Chana, Surat Thani province. Primarily, the explant types for callus induction were investigated, and it was found that zygotic embryo is a suitable explant source, with a high potential of responsive tissue, and lacking browning secretions during culturing. The callus induction process was investigated by culturing zygotic embryos on MS medium supplemented with dicamba concentrations of 1.0, 2.5, and 5.0 mg.L^-1 combined with 200 mg.L^-1 ascorbic acid. The best 74.16 % callus response was obtained with 2.5 mg.L^-1 dicamba. Callus proliferation was good on a medium with the reduced dicamba concentration of 0.5 mg.L^-1, giving the largest 0.41 cm callus size, and the highest 0.141 g callus fresh weight. Embryogenic callus competence was successfully induced at 38.33 % when culturing with 0.1 mg.L^-1 dicamba and 1.0 g.L^-1 casein hydrolysate. Embryogenic calli clump cultivated on MN6 medium developed to somatic embryos in the globular stage at 95.23 %. This suggests that the NO3/NO3+NH4 ratio and vitamin contents in MN6 could promote somatic embryogenesis.

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Author Biography

Duangkhaetita KANJANASOPA, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani 84000

Faculty of Science and Industrial Technology, Prince of Songkla University

References

TK Lim. Edible Medicinal and Non-Medicinal Plants. Vol I. Springer, New York, 2012, p. 396-8.

TA Thorpe. History of plant tissue culture. Mol. Biotechnol. 2007; 37, 169-80.

A Feher TP Pasternak and D Dudits. Transition of somatic plant cells to an embryogenic state. Plant Cell Tiss. Organ. Cult. 2003; 74, 201-28.

AK Patel, T Agarwal, M Phulwaria, V Kataria and NS Shekhawat. An efficient in vitro plant regeneration system from leaf of mature plant of Leptadenia reticulate (Jeewanti): A life giving endangered woody climber. Ind. Crop. Prod. 2014; 52, 499-505.

C Sudhersan, M AboEl-Nil and J Hussain. Tissue culture technology for the conservation and propagation of certain native plants. J. Arid. Environ. 2003; 54, 133-47.

S Chehmalee and S Te-chato. Induction of somatic embryogenesis and plantlet regeneration from cultured zygotic embryo of oil palm. J. Agric. Tech. 2008; 4, 137-46.

JM Al-Khayri. Influence of yeast extract and casein hydrolysate on callus multiplication and somatic embryogenesis of date palm (Phonix dactylifera L.). Sci. Hortic. 2011; 130, 531-5.

AZ Zulkepli, H Jaafar and AHA Rusni. Optimization of sterilization method and callus induction of Salacca glabrescens. In: Proceedings of the International Conference on Biology, Environment and Chemistry IPCBEE, Singapore, 2011.

SC Fernando and CKA Gamage. Abscisic acid induced somatic embryogenesis in immature embryo explants of coconut (Cocos nucifera L.). Plant Sci. 2000; 151, 193-8.

T Murashige and F Skoog. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum. 1962; 15, 473-97.

M Thuzar, A Vanavichit, S Tragoonrung and C Jantasuriyarat. Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis. Acta Physiol. Plant. 2011; 33, 123-8.

V Sarasan, MM Ramsay and AV Roberts. In vitro germination and induction of direct somatic embryogenesis in ‘Bottle Palm’ [Hyophorbe lagenicaulis (L. Bailey) H. E. Moore], a critically endangered Mauritian palm. Plant Cell Rep. 2002; 20, 1107-11.

M Elhiti and C Stasolla. The Use of Zygotic Embryos as Explants for in vitro Propagation: An Overview. In: TA Thorpe and EC Yeung (eds.). Plant Embryo Culture: Methods and Protocols, Methods in Molecular Biology. Springer Science Business, 2011, p 229-55.

S Chehmalee and S Te-chato. Genotypes, physiological ages of zygotic embryos and auxin as affect on germination and callus formation of oil palm. In: Proceeding of the International Conference on Integration of Science & Technology for Sustainable Development, Bangkok, Thailand, 2007, p. 166-9.

VC Lapitan, KLC Nicolas and ET Rasco. Tissue culture technique for clonal propagation of nipa palm (Nypa fruticans Wurmb., Arecaceae) from embryo culture. Asia Life Sci. 2015; 24, 111-25.

C Ulisses, GF Melo-de-Pinna, L Willadino, CC de Albuquerque and TR Camara. In vitro propagation of Heliconia bihai (L.) L. from zygotic embryos. Acta Bot. Bras. 2010; 24, 184-92.

AM Taji and RP Williams. Overview of Plant Tissue Culture. In: AM Taji and RP Williams (eds.). Tissue Culture of Australia. University of New England Press, Australia, 1996, p. 1-5.

S Te-chato and A Hilae. High-frequency plant regeneration through secondary somatic embryogenesis in oil palm (Elaeis guineensis Jacq. var. tenera). J. Agric. Tech. 2007; 3, 345-57.

T Niyakid and S Te-chato. Induction and proliferation of embryogenic cell suspension of oil palm (Elaeis guineensis Jacq. var. tenera) and evaluation of genetic variation by RAPD marker. J. Agric. 2012; 28, 273-83.

B de Touchet, Y Duval and C Pannetier. Plant regeneration from embryogenic suspension culture of oil palm (Elaeis guineensis Jacq.). Plant Cell Rep. 1991; 10, 529-32.

T Srisawat and J Nathinan. Preparation of Vatica diospyroides Symington: An endangered medicinal Dipterocarp of peninsular Thailand by cultures of embryonic axes and leaf-derived calli. Pak. J. Biol. Sci. 2013; 16, 396-400.

HC Wang, JT Chen and WC Chang. Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu. Biol. Plantanum 2006; 50, 279-82.

DA Steinmacher, CR Clement and MP Guerra. Somatic embryogenesis from immature peach palm inflorescence explants: Towards development of an efficient protocol. Plant Cell Tiss. Organ. Cult. 2007; 89, 15-22.

S Sanputawong and S Te-chato. Effect of genotypes of oil palm on callus, embryogenic callus and somatic embryo formation. In: Proceeding of the 1st Mae Fah Luang University International Conference. Chiang Rai, Thailand, 2012.

F Bister-Miel, JL Guignard and C Agier. Glutamine as an active component of casein hydrolysate: its balancing effect on plant cells cultured in phosphorus deficient medium. Plant Cell Rep. 1985; 4, 161-3.

MH Zenk, H El-Shagi and U Schulte. Anthraquinone production by cell suspension cultures of Marinda citrifolia. Planta Med. 1975; 28, 79-101.

T Cardiand and LM Monti. Optimization of callus culture in pea (Pisum sativum). Ann. Fac. Agric. Portici. 1990; 24, 11-8.

A Das and N Mandal. Enhanced development of embryogenic callus in Stevia rebaudiana Bert. by additive and amino acids. Biotechnology 2010; 9, 368-72.

EF George and GJ de Klerk. The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients. In: EF George, MA Hall and GJ de Klerk (eds.). Plant Preparation by Tissue Culture. 3rd ed. Springer, 2008, p. 65-113.

NP Samson, C Campa, LL Gal, M Noirot, G Thomas, TS Lokeswari and A de Kochko. Effect of primary culture medium composition on high frequency somatic embryogenesis in different Coffea species. Plant Cell Tiss. Organ. Cult. 2006; 38, 37-45.

T Thorpe, C Stasolla, EC Yeung, GJ de Klerk, A Roberts and EF George. The Components of Plant Tissue Culture Media II: Organic Additions, Osmotic and pH Effects, and Support Systems. In: EF George, MA Hall and GJ de Klerk (eds.). Plant Preparation by Tissue Culture. 3rd ed. Springer, 2008, p. 115-73.

UB Barwale, HR Kerns and JM Widholm. Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 1986; 167, 473-81.

I Besse, JL Verdeil, Y Duval, B Sotta, R Maldiney and E Miginia. Oil palm (Elaeis guineensis Jacq.) clonal fidelity: endogenous cytokinins and indolacetic acid in embryogenic callus cultures. J. Exp. Bot. 1992; 43, 983-9.

CJ Eeuwens, S Lord, CR Donough, V Rao, G Vallejo and S Nelson. Effects of tissue culture conditions during embryoid multiplication on the incidence of ‘‘mantled’’ flowering in clonally propagated oil palm. Plant Cell Tiss. Organ. Cult. 2002; 70, 311-23.