In vitro Antioxidant Activity of Smaller Size of Coenzyme Q10-Enriched Shell of Ultra-Small Nanostructured Lipid Carriers

Nuttakorn BAISAENG, Daniel PETERS, Michel PROST, Philippe DURAND, Rainer Helmut MÜLLER, Cornelia KECK

Abstract


CoQ10-enriched shells of ultra-small nanostructured lipid carriers (usNLC), or oil with dissolved CoQ10 surrounding a based core of a solid lipid and oil mixture, were successfully produced using a hot high pressure homogenization technique. Then, 2 different concentrations of CoQ10 were loaded into usNLC, to compare the effect of particle size on antioxidant capacity. A particle size of 5.0 % CoQ10-loaded usNLC (about 80 nm) was approximately 2 times larger than 0.5 % CoQ10-loaded usNLC (about 50 nm), and the lightness of the yellow-colored pigment of CoQ10 was observable by the naked eye as regards the decrease in particle size. In addition, the spherical shape of the empty usNLC and CoQ10-enriched shell of usNLC was shown by a transmission electron microscopy (TEM). Interestingly, a 10 times lower CoQ10 loading capacity into usNLC with a smaller size showed a higher antioxidant capacity than a higher CoQ10 loading and a bulk solution of CoQ10, by decreasing DPPH free radical scavenging activity using the DPPH method and increasing the resistance of red blood cells to oxidative damage using a biological Kit Radicaux Libres (KRL) test. Therefore, this study suggests that the smaller particle size of CoQ10-enriched shells of usNLC deserves to be developed and evaluated further in vivo study, in order to prove the antioxidant effects.


Keywords


CoQ10-enriched shell, ultra-small nanostructured lipid carriers, antioxidant, DPPH method, KRL test

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References


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