In vitro Antioxidant Activity of Smaller Size of Coenzyme  Q10-Enriched Shell of Ultra-Small Nanostructured Lipid Carriers

Nuttakorn BAISAENG; Daniel PETERS; Michel PROST; Philippe DURAND; Rainer Helmut MÜLLER; Cornelia KECK

Authors

Nuttakorn BAISAENG School of Pharmaceutical Sciences, University of Phayao, Phayao 56000
Daniel PETERS Institut für Pharmazie, Pharmazeutische Technologie, Biopharmazie and Kosmetik, Freie Universität Berlin, Kelchstraße 31, 12169 Berlin
Michel PROST Kirial International, Laboratoire SPIRAL, Couternon
Philippe DURAND Kirial International, Laboratoire SPIRAL, Couternon
Rainer Helmut MÜLLER Institut für Pharmazie, Pharmazeutische Technologie, Biopharmazie and Kosmetik, Freie Universität Berlin, Kelchstraße 31, 12169 Berlin
Cornelia KECK Applied Pharmacy Division, University of Applied Sciences Kaiserslautern, 66953 Pirmasens

Keywords:

CoQ10-enriched shell, ultra-small nanostructured lipid carriers, antioxidant, DPPH method, KRL test

Abstract

CoQ₁₀-enriched shells of ultra-small nanostructured lipid carriers (usNLC), or oil with dissolved CoQ₁₀ surrounding a based core of a solid lipid and oil mixture, were successfully produced using a hot high pressure homogenization technique. Then, 2 different concentrations of CoQ₁₀ were loaded into usNLC, to compare the effect of particle size on antioxidant capacity. A particle size of 5.0 % CoQ₁₀-loaded usNLC (about 80 nm) was approximately 2 times larger than 0.5 % CoQ₁₀-loaded usNLC (about 50 nm), and the lightness of the yellow-colored pigment of CoQ₁₀ was observable by the naked eye as regards the decrease in particle size. In addition, the spherical shape of the empty usNLC and CoQ₁₀-enriched shell of usNLC was shown by a transmission electron microscopy (TEM). Interestingly, a 10 times lower CoQ₁₀ loading capacity into usNLC with a smaller size showed a higher antioxidant capacity than a higher CoQ₁₀ loading and a bulk solution of CoQ₁₀, by decreasing DPPH free radical scavenging activity using the DPPH method and increasing the resistance of red blood cells to oxidative damage using a biological Kit Radicaux Libres (KRL) test. Therefore, this study suggests that the smaller particle size of CoQ₁₀-enriched shells of usNLC deserves to be developed and evaluated further in vivo study, in order to prove the antioxidant effects.

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