Antibiotic Potency of Extract from Streptomyces Isolated from Terrestrial Soil of Amirthi Forest, India



The aims of the present study were to isolate novel actinomycetes from the terrestrial soil and to evaluate their antibacterial potential. A total of 26 strains of actinomycetes were isolated and screened for antibacterial activity by the well diffusion method. Two of the isolates, VITAM-10 and VITAM-16, possessed a broad spectrum of antibacterial activity against the selected Gram positive and Gram negative ATCC bacterial strains. The isolate VITAM-16 produced a 22 mm zone of inhibition against Proteus vulgaris and VITAM-10 produced 17.6 mm zone of inhibition against Bacillus cereus. VITAM-16 exhibited potent antibacterial activity than VITAM-10. Hence, VITAM-16 was chosen for further studies. The isolate VITAM-16 was subjected to morphologic, cultural and biochemical characterizations and identified to be belonged to the genus Streptomyces. 16S rDNA nucleotide sequence of VITAM-16 showed 97 - 99 % sequences similarity with Streptomyces taxons. The ethyl acetate (EA) extract of VITAM-16 was analyzed by GC-MS, fumaric acid and propanoic acid were found to be the major compounds. The EA extract exhibited an MIC value from 0.06-0.90 mg/ml against tested bacterial pathogens. The results of the present study suggest that VITAM-16 can be explored further for identification of novel antibiotics.


Terrestrial actinomycetes, Streptomyces, secondary metabolites, antibacterial activity

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