Antibiotic Potency of Extract from Streptomyces Isolated from Terrestrial Soil of Amirthi Forest, India

Authors

  • Mani ABIRAMI Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu
  • Krishnan KANNABIRAN Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu

Keywords:

Terrestrial actinomycetes, Streptomyces, secondary metabolites, antibacterial activity

Abstract

The aims of the present study were to isolate novel actinomycetes from the terrestrial soil and to evaluate their antibacterial potential. A total of 26 strains of actinomycetes were isolated and screened for antibacterial activity by the well diffusion method. Two of the isolates, VITAM-10 and VITAM-16, possessed a broad spectrum of antibacterial activity against the selected Gram positive and Gram negative ATCC bacterial strains. The isolate VITAM-16 produced a 22 mm zone of inhibition against Proteus vulgaris and VITAM-10 produced 17.6 mm zone of inhibition against Bacillus cereus. VITAM-16 exhibited potent antibacterial activity than VITAM-10. Hence, VITAM-16 was chosen for further studies. The isolate VITAM-16 was subjected to morphologic, cultural and biochemical characterizations and identified to be belonged to the genus Streptomyces. 16S rDNA nucleotide sequence of VITAM-16 showed 97 - 99 % sequences similarity with Streptomyces taxons. The ethyl acetate (EA) extract of VITAM-16 was analyzed by GC-MS, fumaric acid and propanoic acid were found to be the major compounds. The EA extract exhibited an MIC value from 0.06-0.90 mg/ml against tested bacterial pathogens. The results of the present study suggest that VITAM-16 can be explored further for identification of novel antibiotics.

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Author Biography

Mani ABIRAMI, Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu

Biomolecules and Genetic Division, School of Biosciences and Technology

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Published

2016-10-15

How to Cite

ABIRAMI, M., & KANNABIRAN, K. (2016). Antibiotic Potency of Extract from Streptomyces Isolated from Terrestrial Soil of Amirthi Forest, India. Walailak Journal of Science and Technology (WJST), 14(9), 711–721. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/2038