Antibiotic Potency of Extract from Streptomyces Isolated from Terrestrial Soil of Amirthi Forest, India

Mani ABIRAMI, Krishnan KANNABIRAN

Abstract


The aims of the present study were to isolate novel actinomycetes from the terrestrial soil and to evaluate their antibacterial potential. A total of 26 strains of actinomycetes were isolated and screened for antibacterial activity by the well diffusion method. Two of the isolates, VITAM-10 and VITAM-16, possessed a broad spectrum of antibacterial activity against the selected Gram positive and Gram negative ATCC bacterial strains. The isolate VITAM-16 produced a 22 mm zone of inhibition against Proteus vulgaris and VITAM-10 produced 17.6 mm zone of inhibition against Bacillus cereus. VITAM-16 exhibited potent antibacterial activity than VITAM-10. Hence, VITAM-16 was chosen for further studies. The isolate VITAM-16 was subjected to morphologic, cultural and biochemical characterizations and identified to be belonged to the genus Streptomyces. 16S rDNA nucleotide sequence of VITAM-16 showed 97 - 99 % sequences similarity with Streptomyces taxons. The ethyl acetate (EA) extract of VITAM-16 was analyzed by GC-MS, fumaric acid and propanoic acid were found to be the major compounds. The EA extract exhibited an MIC value from 0.06-0.90 mg/ml against tested bacterial pathogens. The results of the present study suggest that VITAM-16 can be explored further for identification of novel antibiotics.


Keywords


Terrestrial actinomycetes, Streptomyces, secondary metabolites, antibacterial activity

Full Text:

PDF

References


C Olano, C Mendez and JA Salas. Antitumor compounds from marine actinomycetes. Mar. Drugs 2009; 7, 210-48.

S Valli, SS Suvathi, OS Aysha, P Nirmala, KP Vinoth and A Reena. Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pac. J. Trop. Biomed. 2012; 2, 469-73.

HS Chaudhary, J Yadav, AR Shrivastava, S Singh, AK Singh and N Gopalan. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). J. Adv. Pharm. Tech. Res. 2013; 4, 118-23.

A Meklat, N Sabaou, A Zitouni, F Mathieu and A Lebrihi. Halophilic actinomycetes in Saharan soils of Algeria: Isolation, taxonomy and antagonistic properties. Appl. Environ. Microbiol. 2011; 77, 6710-4.

C Jiang and L Xu. Actinomycete diversity in unusual habitats. Actinomycetes 1993; 4, 47-57.

P Manivasagana, J Venkatesana, K Sivakumar and SK Kim. Pharmaceutically active secondary metabolites of marine actinobacteria. Microbiol. Res. 2013; 169, 262-78.

R Subramani and W Aalbersberg. Marine actinomycetes: An ongoing source of novel bioactive metabolites. Microbiol. Res. 2012; 167, 571-80.

A Janardhan, AP Kumar, B Viswanath, DVR Saigopal and G Narasimha. Production of bioactive compounds by actinomycetes and their antioxidant properties. Biotechnol. Res. Int. 2014; 2014, 217030 2014.

ZQ Xiong, JF Wang, YY Hao and Y Wang. Recent advances in the discovery and development of marine microbial natural products. Mar. Drugs 2013; 11, 700-17.

N Kumar, RK Singh, SK Mishra, AK Singh and UC Pachouri. Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria. Int. J. Microbiol. Res. 2010; 2, 12-6.

J Cheng, SH Yang, SA Palaniyandi, JS Han, TM Yoon, TJ Kim and JW Suh. Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968 isolated from agricultural soil. J. Korean Soc. Appl. Biol. Chem. 2010; 53, 545-52.

R Bhatnagar and SK Kim. Immense essence of excellence: Marine microbial bioactive compounds. Mar. Drugs 2010; 8, 2673-701.

R Varghese, S Nishamol, R Suchithra, S Jyothy and AAM Hatha. Distribution and antibacterial activity of actinomycetes from Shola soils of tropical Montane forest in Kerala, South India. J. Environ. 2012; 1, 93-9.

M Abirami, JV Gopal and K Kannabiran. Extraction and identification of antibacterial compound from marine Streptomyces sp. VITAK1 isolated from the coast of Andaman and Nicobar Islands, India. Appl. Biochem. Microbiol. 2015; 51, 406-10.

P Alexander, HI Baumann, K Kleinschmidt, P Ensle, J Wiese, RD Sussmuth and JF Imhoff. Champacyclin, a new cyclic Octapeptide from Streptomyces strain C42 isolated from the Baltic Sea. Mar. Drugs 2013; 11, 4834-57.

MV Arasu, V Duraipandiyan and S Ignacimuthu. Antibacterial and antifungal activities of polyketide metabolite from marine Streptomyces sp. AP-123 and its cytotoxic effect. Chemosphere 2013; 90, 479-87.

AW Baur, WM Kirby, JC Sherris and M Turck. Antibiotic susceptibility testing by a standard single disk method. Am. J. Clin. Path. 1966; 45, 493-6.

D Arijit, S Bhattacharya, AYH Mohammed and SS Rajan. In vitro Antimicrobial activity and characterization of mangrove isolates of Streptomyces: Effective against bacteria and fungi of Nosocomial Origin. Braz. Arch. Biol. Tech. 2014; 57, 349-6.

M Abirami, VG khanna and K Kannabiran. Antibacterial activity of marine Streptomyces sp. isolated from Andaman & Nicobar Islands, India. Int. J. Pharm. Bio. Sci. 2013; 4, 280-6.

MH Farris and JB Oslon. Detection of Actinobacteria cultivated from environmental samples reveals bias in universal primers. Lett. Appl. Microbiol. 2007; 45, 376-81.

M Umasankar, G Kumar, L Karthik and KVB Rao. Exploration of antagonistic actinobacteria from Amirthi forest. Int. J. Current Pharm. Res. 2010; 2, 16-9.

JS Naine, N Nasimunislam, B Vaishnavi, V Mohanasrinivasan and SC Devi. Isolation of soil actinomycetes inhabiting Amrithi forest for the potential source of bioactive compounds. Asian J. Pharm. Clin. Res. 2012; 5, 189-92.

M Khanna, R Solanki and R Lal. Selective isolation of rare actinomycetes producing novel antimicrobial compound. Int. J. Adv. Biotectech. Res. 2011; 2, 357-75.

M Thenmozhi, S Sindhura and K Kannabiran. Characterization of antioxidant activity of Streptomyces species VITTK3 isolated from Puducherry Coast, India. J. Adv. Sci. Res. 2010; 1, 46-52.

C He, B Fu, H Shen, X Jiang and X Wei. Fumaric acid, an antibacterial component of Aloe vera. Afr. J. Biotechnol. 2011; 10, 2973-7.

C Hecer and M Guldas. Effects of lactic acid, fumaric acid and chlorine dioxide on shelf-life of broiler wings during storage. Afr. J. Microbiol. Res. 2011; 23, 3880-3.

RL Podolak, JF Zayas, CL Kastner and DYC Fung. Reduction of bacterial populations on vacuum-packaged ground beef patties with fumaric and lactic acids. J. Food. Prot. 1996; 59, 1037-40.

MN Haque, R Chowdhury, KMS Islam and MA Akbar. Propionic acid is an alternative to antibiotics in poultry diet. Bang. J. Anim. Sci. 2009; 38, 115-22.

Y Okami and K Hotta. Search and Discovery of New Antibiotics, Actinomycetes in Biotechnology. Academic Press, London, 1988, p. 37-67.


Refbacks

  • There are currently no refbacks.




http://wjst.wu.ac.th/public/site/images/admin/image012_400

Online ISSN: 2228-835X

http://wjst.wu.ac.th

Last updated: 12 August 2019