Partially Purified Pigment Extract from Streptomyces A 16-1 Induces Apoptosis of Human Carcinoma of Nasopharynx Cell (KB cells) via the Mitochondrial and Caspase-3 Pathway

Chantarawan SAENGKHAE, Rattanaporn SRIVIBOOL, Rawiwan WATANADILOK, Keiichi ENOMOTO

Abstract


The genus Streptomycetes are very important bacteria which produce secondary metabolites used in clinical cancer therapy and other pharmacological activities. Streptomyces A 16-1 was isolated from coastal soil in Chonburi, Thailand. The objective of this study is to examine the apoptotic effects and mechanisms of the partially purified pigment extract from Streptomyces A 16-1 against human carcinoma of nasopharynx cell (KB cells). The methanol and ethyl acetate extract from the fermentation of ISP2 broth was fractionated by using column chromatography with stationery-phase silica-gel and mobile phase chloroform: methanol mixtures with increasing polarity. Among 9 fractions (Fr), the fractions which contained red pigment were selected and the calculated IC50 values for Fr 5, Fr 6 and Fr 7 were 0.04 ± 0.005, 0.20 ± 0.02, and 0.55 ± 0.05 μg/ml, respectively. The molecular mechanisms of cell death were associated with DNA fragmentation, accumulation of cells in sub-G1 phase, mitochondrial membrane depolarization, and activation of caspase-3, which was significantly diminished by the caspase-3 inhibitor. These first findings demonstrate that the pro-apoptotic effects of these fractions were mediated through the mitochondrial and caspase-3 pathway.


Keywords


Apoptosis, Streptomyces A 16-1, DNA fragmentation, mitochondria, caspase-3

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References


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