Low Cost DNA Molecular Weight Marker: Primer-Directed Synthesis from pGEM-T Easy Vector

Authors

  • Siriporn RIYAJAN School of Science, Walailak University, Nakhon Si Thammarat 80161, Thailand
  • Wiparat YENTUA Center of Excellence on Agricultural Biotechnology (Ag-BIO/PERDO-CHE), Bangkok 10900, Thailand
  • Jaros PHINCHONGSAKULDIT School of Science, Walailak University, Nakhon Si Thammarat 80161, Thailand

Keywords:

DNA molecular weight marker, marker primer-directed synthesis, pGEM-T Easy vector

Abstract

A low cost DNA molecular weight marker was produced by a marker primer-directed synthetic method using pGEM-T Easy vector as the DNA template. Seven primers were used to amplify eight different DNA fragments, which were 150, 300, 375, 500, 700, 1,000, 1,200 and 1,625 bp, from bacterial culture containing pGEM-T Easy vector. Polymerase chain reactions (PCR) for all marker loci required the same optimal annealing temperature, which allowed all the PCR to be completed in a single run. To obtain the molecular weight marker, the PCR product of each locus was mixed together and directly used as marker without any further purification. This custom made molecular weight marker was found to be approximately 17 to 49 times less expensive than other commercial 100 bp DNA ladder markers.


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Published

2011-11-06

How to Cite

RIYAJAN, S., YENTUA, W., & PHINCHONGSAKULDIT, J. (2011). Low Cost DNA Molecular Weight Marker: Primer-Directed Synthesis from pGEM-T Easy Vector. Walailak Journal of Science and Technology (WJST), 8(2), 187–192. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/20

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Research Article

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