Antifungal Activities of Some Pleurotus Species (Higher Basidiomycetes)

Mustafa Nadhim OWAID, Sajid Salahuddin Saleem AL SAEEDI, Idham Ali ABED, Pedram SHAHBAZI, Vikineswary SABARATNAM

Abstract


This study investigated the antifungal activities of 4 Pleurotus spp. (oyster mushrooms) against 3 pathogenic fungi: Trichoderma harzianum, Verticillium sp., and Pythium sp. The antifungal activity of the mycelia of oyster mushrooms was tested against 3 pathogenic fungi grown in fresh PDA medium using dual culture testing. The antifungal activities of the culture filtrates of oyster mushrooms were tested in solid and liquid media. The highest inhibition activity, of 55.56 %, was observed with P. salmoneostramineus against Verticillium sp., while the least percent inhibition of 46.15 % was with the mycelia of Pleurotus ostreatus against T. harzianum. Then, Pleurotus ostreatus grew over the mycelia of Pythium sp. in 5.33 days, while P. cornucopiae var. citrinopileatus mycelia did not grow over the mycelia of Verticillium sp. and T. harzianum. Antifungal activity of the culture filtrate of Pleurotus spp. on agar media was variable. The highest inhibition was 12.33 %, followed 11.60 % for P. salmoneostramineus and P. ostreatus against Verticillium sp. and T. harzianum, respectively, whereas lower inhibitions at 1.34 and 1.75 % were observed for the culture filtrates of P. cornucopiae and P. ostreatus against Verticillium sp. and Pythium sp., respectively. In liquid media, the highest inhibition was 55 and 50 % with P. ostreatus and P. salmoneostramineus culture filtrates against T. harzianum and Verticillium sp., respectively, whereas the lowest inhibition was 3.33 % in the case of P. ostreatus against T. harzianum.


Keywords


Culture filtrate, inhibition, mycelia, pathogenic fungi, Pleurotus spp.

Full Text:

PDF

References


SP Wasser and AL Weis. Medicinal properties of substances occuring in higher Basidiomycetes mushrooms: Current perspectives (Review). Int. J. Med. Mushr. 1999; 1, 31-62.

H Sagakami, T Aohi, A Simpson and S Tanuma. Induction of immunopotentiation activity by a protein-bound polysaccharide PSK. Anticancer Res. 1991, 11, 993-1000.

MN Owaid. 2013, Testing Efficiency of Different Agriculture Media in Growth and Production of Four Species of Oyster Mushroom Pleurotus and Evaluation the Bioactivity of Tested Species. Ph.D. Dissertation. University of Anbar, Ramadi, Iraq.

MN Owaid, SSS Al-Saeedi and IA Al-Assaffii. Antimicrobial activity of mycelia of oyster mushroom species (Pleurotus spp.) and their liquid filtrates (in vitro). J. Med. Bioeng. 2015; 4, 376-80.

MN Owaid, SSS Al-Saeedi and IA Al-Assaffii. Impact palm date fibers (fibrillum) and sawdust extract on mycelial growth rate of four species of Pleurotus. J. Tikrit. Univ. Agric. Sci. 2014; 14, 1-7.

MN Owaid, BM Nassar, AM Abed and AM Turki. Effect of cellulosic matter and container size on cultivation and yield of oyster mushroom Pleurotus ostreatus. J. Med. Herb Ethnomed. 2015; 1, 59-63.

MN Owaid, AM Abed and BM Nassar. Recycling cardboard wastes to produce blue oyster mushroom Pleurotus ostreatus in Iraq. Emir. J. Food Agric. 2015; 27, 537-41.

MN Owaid, IA Abed and SS Al-Saeedi. Using of date palm fiber mixed with other lignocelluloses toward Pleurotus ostreatus (Higher Basidiomycetes) cultivation. Emir. J. Food Agric. 2015; 27, 556-61.

MN Owaid, SSS Al-Saeedi, V Sabaratnam, IAA Al-Assaffii and J Raman. Growth performance and cultivation of four oyster mushroom species on sawdust and rice bran substrates. J. Adv. Biotech. 2015; 4, 424-9.

A Castle, D Speranzini, N Rghei, G Alm, D Rinker and J Bissett. Morphological and molecular identification of Trichoderma isolates on North American Mushroom Farms. Appl. Environ. Microbiol. 1998; 64, 133-7.

P Angelini, R Pagiotti and P Granetti. Effect of antimicrobial activity of Melaleuca alternifolia essential oil on antagonistic potential of Pleurotus species against Trichoderma harzianum in dual culture. World J. Microbiol. Biotech. 2008; 24, 197-202.

GM Abdel-Fattah, YM Shabana, AE Ismail and YM Rashad. Trichoderma harzianum: A biocontrol agent against Bipolaris oryzae. Mycopathologia 2007; 164, 81-9.

S Badalyan, OS Isikhuemhen and MG Gharibyan. Antagonistic/antifungal activities of medicinal mushroom Pleurotus tuberregium (Fr.) singer (Agaricomycetideae) against selected filamentous fungi. Int. J. Med. Mushr. 2008; 10, 155-62.

JK Yang, BK Lim, G Alm and DL Rinker. Response of Trichoderma and Pleurotus to extracts of cypress leaves. Int. Soc. Mushr. Sci. 2004; 16, 547-52.

AA Hassan, AE Al-Kurtany, EM Jbara and KF Saeed. Evaluation of an edible mushroom Pleurotus sp. efficiency against to plant pathogens: Nematodes and soil fungi. In: Proceedings of the 5th Scientific Conference of College of Agriculture, Tikrit University, Iraq, 2011, p. 431-47.

P Angelini, R Pagiotti, R Venanzoni and B Granetti. Antifungal and allelopathic effects of Asafoetida against Trichoderma harzianum and Pleurotus spp. Allelopath. J. 2009; 23, 357-68.

A Borhani, SM Badalyan, NN Garibyan, SA Mosazadeh and E Yasari. Flammulina velutipes (Curt.: Fr.) singer: An edible mushroom in Northern Forest of Iran and its antagonistic activity against selected plant pathogenic fungi. Int. J. Biol. 2011; 3, 162-7.

H Wang and TB Ng. Eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom Pleurotus eryngii. Peptides 2004; 25, 1-5.

MN Owaid, SSS Al-Saeedi and IA Abed. Mineral elements of white, grey, yellow and pink oyster mushrooms (Higher Basidiomycetes). J. Food 2015; 40, 319-26.

G Gregori, M Svagelj and J Pohleven. Cultivation techniques and medicinal properties of Pleurotus spp. Food Tech. Biotech. 2007; 45, 236-47.

E Vamanu. Biological activities of the polysaccharides produced in submerged culture of two edible Pleurotus ostreatus mushrooms. J. Biomed. Biotech. 2012; 2012, 565974.

V Parameswari and P Chinnaswamy. An in vitro study of the inhibitory effect of Pleurotus florida a higher fungi on human pathogens. J. Pharm. Res. 2011; 4, 1948194-9.

K Okamoto, S Narayama, A Katsuo, I Shigematsu and H Yanase. Biosynthesis of p-Anisaldehyde by the white-rot basidiomycete Pleurotus ostreatus. J. Biosci. Bioeng. 2002; 93, 207-10.

ST Chang and PG Miles. Mushrooms Cultivation, Nutritional Value, Medicinal Effect and Enviromental Impact. 2nd ed, CRC Press LLC, USA, 2004, p. 381.

JB Akinyele, S Fakoya and CF Adetuyi. Anti-growth factors associated with Pleurotus ostreatus in a submerged liquid fermentation. Malays. J. Microbiol. 2012; 8, 135-40.

MN Owaid, J Raman, H Lakshmanan, SSS Al-Saeedi, V Sabaratnam and IAA Al-Assaffii. Mycosynthesis of silver nanoparticles from Pleurotus cornucopiae var. citrinopileatus and its inhibitory effects against Candida sp. Mater. Lett. 2015; 153, 186-90.

DM Weller, BX Zhang and RJ Cook. Application of rapid screening test for selection of bacteria suppression to take-all wheat. Plant Dis. 1985; 69, 710-3.

OI Nwanneka, AO Ogbe and O Olufunke. Effect of the mycelial of Ganoderma lucidum on human pathogenic bacteria. Int. J. Biol. 2011; 3, 111-4.

CH Dickinson and AM Skidmore. Interactions between germinating spores of Septoria nodorum and phylloplane fungi. Trans. Br. Mycol. Soc. 1976; 66, 45-56.

L Ludikhuyze, AV Loey, Indrawati and M Hendrickx. Combined High Pressure Thermal Treatment of Foods. In: P Richardson (ed.). Thermal Technologies in Food Processing. CRC Press, New York, USA, 2001, p. 266-84.

LN Ofodile, NU Kokubum, ORJ Grayer, OT Ogundipe and MSJ Simmonds. Antimicrobial activity of some Ganoderma species from Nigeria. Phytotherapia Res. 2005; 19, 210-3.

Y Patel, R Naraian and VK Singh. Medicinal properties of Pleurotus species (oyster mushroom): A review. World J. Fung. Plant Biol. 2012; 3, 1-12.

M Akyuz and S Kirbag. Antimicrobial activity of Pleurotus eryngii var. ferulae grown on various agro-wastes. EurAsian J. BioSci. 2009; 3, 58-63.

JK Liu. N-containing compounds of macromycetes. Chem. Rev. 2004; 105, 2723-44.

M Muskhazli, QZ Faridah, R Salfarina, TN Farizan and I Nalisha. The evidence of non n-glycan linked mannose in exochitinase 42kDa, from Trichoderma harzianum BIO10671 glycosylation. Malays. J. Microbiol. 2006; 2, 37-41.

JH Kim, SJ Kim, HR Park, JI Choi, YC Ju, KC Nam, SJ Kim and SC Lee. The different antioxidant and anticancer activities depending on the color of oyster mushrooms. J. Med. Plant Res. 2009; 3, 1016-20.

GW Dekan. Introduction to Modern Mycology. 2nd ed. University of Salah ad Din. Dar Alhekma for Printing and Publishing. Erbil, Iraq, 1983, p. 130-314.


Refbacks

  • There are currently no refbacks.




http://wjst.wu.ac.th/public/site/images/admin/image012_400

Online ISSN: 2228-835X

http://wjst.wu.ac.th

Last updated: 17 May 2019