Use of Antifungal Metabolite from Trichoderma virens for Controlling Chinese Kale Leaf Spots Caused by Alternaria brassicicola


  • Warin INTANA School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161
  • Taksin SUWANNO School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161
  • Chiradej CHAMSWARNG Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140


Antifungal metabolite, Alternaria brassicicola, Chinese kale disease, Biological control, Trichoderma virens


A Total of 13 isolates of Trichoderma virens were isolated from soil samples collected from 9 different sites. All isolates inhibited the mycelial growth of Alternaria brassicicola that causes Chinese kale leaf spot. There were two strains, T-ST-01 and T-NST-01, that gave high values of inhibition, 72.5 and 67.0%, respectively. Strains T-ST-01 and T-NST-01 were used to evaluate antifungal properties using dialysis membrane technique testing with A. brassicicola. The results showed that mycelial growth of A. brassicicola was completely inhibited. Antifungal metabolite was extracted and tested against germination of A. brassicicola spores. The results showed that spores of A. brassicicola germinated 9.4% compared with 98.5% for the control. Use of antifungal metabolite was effective both in laboratory and glasshouse conditions. Severity of the disease was significantly (P<0.05)reduced compared with the control treatment (2% methanol). Disease severity of Chinese kale treated with antifungal from extracted T-ST-01 were 22.5 and 32.0% when measured in laboratory and glasshouse conditions, respectively, while the severity in the control treatment was found to be 89.5 and 84.5%, respectively.


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How to Cite

INTANA, W., SUWANNO, T., & CHAMSWARNG, C. (2011). Use of Antifungal Metabolite from Trichoderma virens for Controlling Chinese Kale Leaf Spots Caused by Alternaria brassicicola. Walailak Journal of Science and Technology (WJST), 2(1), 1–9. Retrieved from



Research Article