Histological Examination of Callogenesis in Bisected Protocorm Culture of Pigeon Orchid (Dendrobium crumenatum Swartz)

Paveena KAEWUBON; Upatham MEESAWAT

Authors

Paveena KAEWUBON Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla 90112
Upatham MEESAWAT Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla 90112

Keywords:

Bisected protocorm, Dendrobium crumenatum, nodular callus, subepidermal layer

Abstract

Callogenesis is a prerequisite step for the initiation of callus-mediated plant regeneration. Three-month-old protocorms of Dendrobium crumenatum Swartz (pigeon orchid) were bisected transversely and cultured on modified Vacin and Went (VW) solid medium containing 20 g L^-1 sucrose, 2 g L^-1 Phytagel added with various concentrations of 1-naphthaleneacetic acid (NAA) (0, 0.1, 0.5 mg L^-1) and 6-benzyladenine (BA) (0, 1, 2 mg L^-1). The highest percentage of callus formation was obtained from the explant cultured on a modified VW supplemented with 0.5 mg L^-1 NAA and 1 mg L^-1 BA. The callus originated from the subepidermal layer of the bisected protocorm and subsequently formed nodular structure after culture for 8 weeks. The callus mass contained small isodiametric cells with prominent nuclei and nucleoli within dense cytoplasm.

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Author Biographies

Paveena KAEWUBON, Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla 90112

Department of Biology, Faculty of Science

Upatham MEESAWAT, Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla 90112

Department of Biology, Faculty of Science

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