Direct Somatic Embryo Formation from Roots of In Vitro-Seedlings of Oil Palm (Elaeis Guineesis Jacq.)

Stapaporn KERDSUWAN, Sompong TE-CHATO


The induction of somatic embryos (SEs) directly from the roots of oil palm plantlets raised in vitro-seedling and origin of somatic embryos. SEs were directly induced from the roots of oil palm plantlets regenerated from immature oil palm fruit (3 months after pollination: 3MAP) and provided by Golden Tenera Company. The plants were cultured on OPCM medium supplemented with    3 % sucrose, 200 mg/l ascorbic acid and 0.5 mg/l NAA. The culture was maintained under light conditions at an intensity of 15 µmol/m2/s, 14 h photoperiod, 28±1 °C for 2 months. Histological studies revealed that SEs were initiated from epidermal cells and a few parenchymatous cells. Parenchyma cells had a large nucleus, dense cytoplasm with a broad basal area in contact with maternal tissue. Those cells underwent rapid cell division to form proembryonic mass (PEM) and finally developed into SEs through somatic embryogenesis (SE). The highest number of SEs was 1.2 SEs/root and the percentage of SE formation was recorded to be 80. The present study confirms direct SE formation from roots and describes the origin of SE.



Oil palm, somatic embryogenesis, in vitro-seedling, proembryonic mass (PEM), root

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MK Rajesh, E Radha, A Karun and VA Parthasarathy. Plant regeneration from embryo-derived callus of oil palm-the effects of exogenous polyamines. Plant Cell. Tiss. Org. Cult. 2003; 75, 41-7.

DA Steinmacher, NG Krohn, ACM Dantas, VM Stefenon, CR Clement and MP Guerra. Somatic embryogenesis in peach palm using the thin cell layer technique: Induction, morphohistological aspects and AFLP analysis of somaclonal variation. Ann. Bot. 2007; 100, 699-709.

VM Jimenez. Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul. 2005; 47, 91-110.

M Thuzar, A Vanavichit, S Tragoonrung and C Jantasuriyarat. Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis. Acta Physiol. Plant. 2011; 33, 123-8.

YJ Pena-Ramirez, I Garcia-Shesena, A Hernandez-Espinoza, A Dominguez-Hernandez, FA Barredo-Pool, JA Gonzalez-Rodriguez and ML Robert. Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]. Plant Cell. Tiss. Org. Cult. 2011; 105, 203-9.

S Te-chato, A Hilae and I Yeedum. Histological study on oil palm of somatic embryos development as affected by sources of leaf explants and auxin. J. Agr. Sci. 2003; 36, 243-50.

S Te-chato. Callus induction from cultured zygotic embryo of oil palm subsequent to plantlet regeneration. Songklanakarin J. Sci. Tech. 1998; 20, 1-6.

C Jourdand and H Rey. Modelling and simulation of the architecture and development of the oil-palm (Elaeis guineensis Jacq.) root system. Plant Soil. 1997; 190, 217-33.

P Ainsey, G Collins and M Sedgley. Adventitious shoot regeneration from leaf explants of almond Prunus dulcis Mill. In Vitro Cell. Dev. Biol. 2000; 36, 470-4.

G Vengadesan, A Ganapathi, RP Anand and VR Anbazhagan. In vitro organogenesis and formation in Acacia sinuate. Plant Cell. Tiss. Org. Cult. 2000; 61, 23-8.

T Murashige and F Skoog. A revised medium for rapid growth and bioassay with tobacco. J. Plant Physiol. 1962; 15, 473-97.

E Corredoira, A Ballester and AM Vieitez. Proliferation, maturation and germination of Castanea sativa Mill. Somatic embryos originated from leaf explants. Ann. Bot. 2003; 92, 120-36.

G Lloyd and B McCown. Commercially-feasible micropropagation of mountain laural, Kalmia latifolia by use shoot-tip culture. Int. Plant Propagator’s Soc. 1980; 30, 421-7.

T Takuya, M Yasuyu, S Makiko, H Chitose, Y Kensuke, K Haruki, S Yasuhiro and K Hisato. Evaluation of basal media for micropropagation of four highbush blueberry cultivars. Sci. Hortic. 2008; 119, 72-4.

V Heedchim. 2014, Molecular markers and flow cytometry for identification of abnormal oil palm plantlet in vitro (in Thai). M. Sc. Thesis. Prince of Songkla University, Songkhla, Thailand.

MD Rosa, C Liliana, G Paola and P Paola. Callus formation and embryogenesis with leaf explants of Aesculus hippocustanum L. J. Plant Physiol. 1986; 126, 93-6.

DJ James, AJ Passey and SB Malhotra. Organogenesis in callus derived from stem and leaf tissues of apple and cherry rootstocks. Plant Cell. Tiss. Org. Cult. 1984; 3, 333-41.

JA Russell and BH McCown. Culture and regeneration of Populus leaf protoplasts isolated from non seedling tissue. Plant Sci. 1986; 46, 133-42.

YBCJ Rosa and MC Dornelas. In vitro regeneration and de novo differentiation of secretory trichomes in Passiflora foetida L. (Passifloraceae). Plant Cell. Tiss. Org. Cult. 2012; 108, 91-9.

DA Johansen. Plant Microtechnique. McGraw Hill, London, UK, 1940.

P Vankatachalam, P Kavikishore, N Geetha, M Thangavely and N Jayabalan. A rapid protocol for somatic embryogenesis from immature leaflets of groundnut (Arachis hypogaea L.). In Vitro Cell. Dev. Biol. 1999; 35, 409-12.

J Qiao, H Kuroda, T Hayashi and F Sakai. Efficient plantlet regeneration from protoplasts isolated from suspension cultures of poplar (Populus alba L.). Plant Cell Rep. 1998; 17, 201-5.

DE Wolfe, P Eck, C Chin and K Chin. Evaluation of seven media for micropropagation of highbush blueberry. Hort. Sci. 1983; 18, 703-5.

M Paques, J Bercetche, G Bruneau, JM Bregeon, A Thivolle-Cazat and P Bonduelle. Genetic engineering for poplar improvement. CR Acad. Agric. 1995; 88, 153-66.

MK Rajesh, E Redha, A Karun and VA Parthasarathy. Plant regeneration from embryo-derived callus of oil palm-the effects of exogenous polyamines. Plant Cell Rep. 2003; 75, 41-7.

K Vankatech, RA Roja, N Baburao and G Padmaja. Effect of auxins and auxin polar transport inhibitor (TIBA) on somatic embryogenesis in groundnut (Arachis hypogaea L.). Afr. J. Plant Sci. 2009; 3, 288-93.

T Fujimura and A Komomine. Effect of various growth regulators on the embryogenesis in a carrot cell suspension culture. Plant Sci. Lett. 1975; 5, 359-64.

P Vankatachalam, P Kavikishore, N Geetha, M Thangavely and N Jayabalan . A rapid protocol for somatic embryogenesis from immature leaflets of groundnut (Arachis hypogaea L.). In Vitro Cell. Dev. Biol. 1999; 35, 409-12.

R Lehminger-Mertens and HJ Jacobse. Plant regeneration from pea protoplast via somatic embryogenesis. Plant Cell Rep. 1989; 8, 379-82.

L Saenz, A Azpeitia and B Chue-Armendariz. Morphological and histological changes during somatic embryo formation from coconut plumule explants. In Vitro Cell. Dev. Biol. 2006; 42, 19-25.

SC Fernando, JL Verdeil, V Hocher, LK Weerakoon and K Hirimburegama. Histological analysis of plant regeneration from plumule explants of Cocos nucifera. Plant Cell. Tiss. Org. Cult. 2003; 72, 281-4.

RIP Perera, V Hocher, JL Verdeil, S Doulbeau, DMD Yakandawala and LK Weerakoon. Unfertilises ovary: a novel explants for coconut (Cocos nucifera L.) somatic embryogenesis. Plant Cell Rep. 2007; 26, 21-8.

R Pierik. Cultivo in vitro de las plantas superiors. Ediciones Mundi-Prensa, Spain, 1991.

C Ramage and R Williams. Mineral nutrition and plant morphogenesis. In Vitro Cell. Dev. Biol. 2002; 38, 116-24.


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