Effect of Modified Zarrouk’s Medium on Growth of Different Spirulina Strains


  • Chandrasekaran RAJASEKARAN School of Bio Sciences and Technology, VIT University, Tamilnadu
  • C. P. Mohammed AJEESH School of Bio Sciences and Technology, VIT University, Tamilnadu
  • Sundaramoorthy BALAJI School of Bio Sciences and Technology, VIT University, Tamilnadu
  • Mohan SHALINI School of Bio Sciences and Technology, VIT University, Tamilnadu
  • Ramamoorthy SIVA School of Bio Sciences and Technology, VIT University, Tamilnadu
  • Ranjan DAS Department of Crop Physiology, Assam Agriculture University, Assam
  • Devanand P. FULZELE Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Maharastra
  • Thiagarajan KALAIVANI School of Bio Sciences and Technology, VIT University, Tamilnadu


Spirulina strains, doubling time, growth response, specific growth rate, chlorophyll A


The effect of modified Zarrouk’s medium on the growth response of 6 different Spirulina strains was evaluated. Specific growth rate, doubling time, mean daily division rate, biomass, and chlorophyll-A contents were analyzed. Growth patterns of these strains were monitored continuously for 40 days. The results revealed significant differences in the growth parameters for different strains. S. platensis (SP-6) and S. platensis (CCMB) showed the maximum specific growth rates (µ = 6.1, µ = 5.8), doubling times (Td = 6.93, Td = 6.87), mean division rates (k = 0.27, k = 0.23) biomasses (5.1, 5.0 g/l) and chlorophyll A contents (78, 65 µg/ml) respectively, when compared with the other strains used in this study. Therefore, S. platensis (SP-6) and S. platensis (CCMB) strains can be suggested for large scale commercial cultivation with modified Zarrouk’s medium. This provides the basis of a low cost medium for cultivating Spirulina, which is known to be a promising microalgae with several benefits.



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Author Biography

Chandrasekaran RAJASEKARAN, School of Bio Sciences and Technology, VIT University, Tamilnadu

School of Bio Sciences and Technology,

VIT University,



MG Mustafa, T Umino and H Nakagawa. The effect of Spirulina feeding on muscle protein deposition in red sea bream, Pagrus major. J. Appl. Phycol. 1994; 10, 141-5.

LM Colla, CO Reinehr, C Reichert and JAV Costa. Production of biomass and nutraceutical compounds by Spirulina platensis under divergent temperature and nitrogen regimes. Bioresource Technol. 2007; 98, 1489-93.

A Belay. The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. J. Am. Nutraceut. Assoc. 2002; 5, 27-48.

F Hong, L Ming, Y Sheng, L Zhanxia, W Yongquan and L Chi. The antihypertensive effect of peptides: A novel alternative to drugs? Peptides 2008; 29, 1062-71.

T Hirahashi, M Matsumoto, K Hazeki, Y Saeki, M Ui and M Seya. Activation of the human innate immune system by Spirulina: Augmentation of interferon production and NK cytotoxicity by oral administration of hot water extract of Spirulina platensis. Int. Immunopharmacol. 2002; 2, 423-34.

B Bermejo, PE Enrique and VDF Angelma. Neuroprotection by Spirulina platensis protean extract and phycocyanin against iron-induced toxicity in SH-SY5Y neuroblastoma cells. Toxicol. Vitro 2008; 22, 1496-502.

A Vonshak and L Tomaselli. Arthrospira (Spirulina): Systematics and Ecophysiology. In: BA Whitton and M Potts (eds.). The Ecology of Cyanobacteria, Kluwer Academic Publishers, The Netherlands, 2000, p. 505-22.

N Simsek, A Karadeniz, Y Kalkan, O Keles and B Unal. Spirulina platensis feeding inhibited the anemia and leucopenia induced lead and cadmium in rats. Hazard. Mater. 2009; 164, 1304-9.

S Balaji, T Kalaivani and C Rajasekaran. Bio sorption of zinc and nickel and its effect on growth on different Spirulina strains. Clean Soil Air Water 2014; 42, 507-12.

O Keskinkan, O Isik, T Yilmaz, B Balci, LH Uslu and CB Ersu. Simultaneous growth of Spirulina platensis and removal of Hardness in Van Lake Water. Clean Soil Air Water 2012; 40, 34-8.

DEO Macl, MPC Monteiro, PG Robbs and SGF Leite. Growth and chemical composition of Spirulina maxima and Spirulina platensis biomass at different temperatures. Aquacult. Int. 1999; 7, 261-75.

L Binaghi, A Del borghi, A Lodi, A Converti and MD Borghi. Batch and fed-batch uptake of carbon dioxide by Spirulina platensis. Process. Biochem. 2003; 38, 1341-6.

A Vonshak. Spirulina platensis (Arthrospira). Physiology, Cell-Biology and Biotechnology. Taylor & Francis, London, 1997, p. 131-58.

C Zarrouk. 1966, Contribution a l’etude d’une Cyanobacterie: Influence de Divers Facteurs Physiques et Chimiques sur la Croissanceet la Photosynthese de Spirulina maxima (Setchell et Gardner) Geitler. Ph. D. Thesis. University of Paris, France.

P Dalgaard and K Koutsoumanis. Comparison of maximum specific growth rates and lag times estimated from absorbance and viable count data by different mathematical models. J. Microbiol. Meth. 2001; 43, 183-94.

PR Pai, A Manasa, T Kalaivani, CPM Ajeesh, C Rajasekaran and BN Prasad. Simplified Cost Effective Media Variants for the Rapid Culture of Spirulina platensis. Recent Advances in Biotechnology, Excel India Publishers, New Delhi, 2008, p. 1-129.

HW Jannasch and T Egli. Microbial growth kinetics: A historical perspective. Antonie Leeuwenhoek 1993; 63, 213-24.

B Raoof, BD Kaushik and R Prasanna. Formulation of a low cost medium for mass production of Spirulina. Biomass Bioenerg. 2006; 30, 537-42.

FF Madkour, AE Kamil and HS Nasr. Production and nutritive value of Spirulina platensis in reduced cost media. Egypt. J. Aquat. Res. 2012; 38, 51-7.

RJ Ritchie. Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosyn. Res. 2006; 89, 27‐41.

S Bhattacharya and MK Shivaprakash. Evaluation of three Spirulina species grown under similar conditions for their growth and biochemicals. J. Sci. Food. Agric. 2004; 85, 333-6.

HK Madhyastha and TM Vatsala. Pigment production in Spirulina fusiform is in different photophysical conditions. Biomol. Eng. 2007; 24, 301-5.

GZ de Caire, JL Parada, MC Zaccaro and MMS de Cano. Effect of Spirulina platensis biomass on the growth of lactic acid bacteria in milk. World J. Microbiol. Biotechnol. 2000; 16, 563-5.

CO Rangel-Yagui, EDG Danesi, JCM Carvalho and S Sato. Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process. Bioresource Technol. 2004; 92, 133-41.

RP Anupama. Value-added food: Single cell protein. Biotechnol. Adv. 2000; 18, 459-79.

T Gireesh, A Jayadeep, KN Rajasekharan, VP Menon, M Vairamany, G Tang, PP Nair and PR Sudhakaran. Production of deuterated β-carotene by metabolic labelling of Spirulina platensis. Biotechnol. Lett. 2001; 23, 447-9.

YK Lee. Commercial production of microalgae in the Asia Pacific rim. J. Appl. Phycol. 1997; 9, 403-11.

DM Li and YZ Qi. Spirulina industry in China: Present status and future prospects. J. Appl. Phycol. 1997; 9, 25-8.

N Subhasha, P Monika and S Rupali. Effect of nitrogen on growth and lipid content of chlorella pyrenoidosa. Am. J. Biochem. Biotechnol. 2011; 7, 124-9.

Olivia, Available at: http://www.differencebetween.com/difference-between-sodium-and-vs-potassium, accessed May 2011.




How to Cite

RAJASEKARAN, C., AJEESH, C. P. M., BALAJI, S., SHALINI, M., SIVA, R., DAS, R., FULZELE, D. P., & KALAIVANI, T. (2015). Effect of Modified Zarrouk’s Medium on Growth of Different Spirulina Strains. Walailak Journal of Science and Technology (WJST), 13(1), 67–75. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/1416



Research Article