Fungus-Mediated Synthesis of Silver Nanoparticles and Their Activity against Gram Positive and Gram Negative Bacteria in Combination with Antibiotics

Krishna GUDIKANDULA; Ram Prasad METUKU; Shiva Krishna PABBA; Shesha Venkata Sathya Siva Surya Laxmi Hima Bindu NIDADAVOLU; Samatha BURRA; Singara Charya Maringanti ALHA

Authors

Krishna GUDIKANDULA Department of Microbiology, Kakatiya University, Warangal 506009
Ram Prasad METUKU Department of Microbiology, Kakatiya University, Warangal 506009
Shiva Krishna PABBA Department of Microbiology, Kakatiya University, Warangal 506009
Shesha Venkata Sathya Siva Surya Laxmi Hima Bindu NIDADAVOLU Department of Microbiology, Kakatiya University, Warangal 506009
Samatha BURRA Department of Microbiology, Kakatiya University, Warangal 506009
Singara Charya Maringanti ALHA Department of Microbiology, Kakatiya University, Warangal 506009

Keywords:

White rot fungi, silver nanoparticles, SEM, antibiotics, antimicrobial activity

Abstract

Silver nanoparticles were synthesized biologically from the white rot fungus, Schizophyllum radiatum and its synergistic antibacterial activity with ceftriaxone and streptomycin was studied. The nanoparticles were characterized using UV-Vis spectroscopy and Scanning Electron Microscopy (SEM). In the UV-Vis spectrum the peak at 420 nm confirmed the synthesis of silver nanoparticles and SEM studies revealed that the silver nanoparticles have different shapes and sizes. Particle size analysis revealed a range between 10 - 40 nm with a mean diameter of 14.5 nm, suggesting the production of different-sized nanoparticles. The antibacterial activity of the silver nanoparticles conjugated with antibiotics was assessed using agar well diffusion with 2 gram positive bacteria Staphylococcus aureus, Bacillus subtilis, and 2 gram negative bacteria Escherichia coli and Pseudomonas aeruginosa. The antibacterial activity of ceftriaxone and streptomycin were evaluated with diameter of inhibition zones for the test bacteria. The antibacterial activity of ceftriaxone and streptomycin increased significantly in presence of Ag-NPs. The maximum antibacterial activity was observed when ceftriaxone and streptomycin conjugated with Ag-NPs. The applications of Ag-NPs and antibiotics together improved their efficiency, reduced side effects and also their cost.

doi:10.14456/WJST.2015.67

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