Effect of Feeding Soybean and Palm Blended Oil on Laying Performance and Egg Quality


  • Niwat MUANGKEOW Division of Animal Production Technology, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161, Thailand


Palm olein oil, soybean oil, laying hen, egg yolk fatty acids


The effect of blended dietary oil on the laying performance of hens and the fatty acid profiles of eggs produced by 21 to 40 wk old Dekalb Brown laying hens were investigated. The experimental design was a completely randomized design using a 4 × 2 factorial arrangement with one control. The different oil sources did not influence egg production, egg weight, egg mass, feed intake, feed efficiency, Haugh unit or egg shell thickness. However lipid profile of the egg yolk changed as a function of dietary lipid sources. The fatty acid composition of egg yolks produced by the laying hens was analyzed. The fatty acid profile of eggs can be modified by varying the lipid composition of their diet. High inclusion levels of dietary palm olein oil (POO) or blended oil (POO plus soybean oil - SBO) concentrations of total saturated fatty acid (SFA) in egg yolks decreased, with SFA = 24.8167 - 0.63Oil, r2 = 0.66 (P < 0.05). Eggs laid by hens fed the diet containing a high portion of soybean oil had a large amount of n-6 polyunsaturated fatty acids (PUFA), whereas egg laid by hens fed the diet containing POO or blended oil 25 % POO plus 75 % SBO at 1.5 % level of inclusion had a high percentage of n-3 PUFA and n-9 family. The addition of blended palm olein oil plus soybean oil decreased the concentration of saturated fatty acids while increased the concentrations of monounsaturated fatty acid, a-linolenic and docosahexaenoic acid (DHA). It did not promote the enrichment of the eggs with PUFA.


Download data is not yet available.


Metrics Loading ...


KC Klasing. Comparative Avian Nutrition, CAB International, New York, 1998.

BA Watkins. Importance of essential fatty acids and their derivatives in poultry. J. Nutr. 1991; 121, 1475-85.

BA Watkins. Biochemical and physiological aspects of polyunsaturates. Poult. And Avian Rev. 1995; 6, 1-18.

RE Newman. 2000, Modulation of Avian Metabolism by Dietary Fatty Acids, Ph. D. Dissertation. The University of Sydney, Australia.

NRC. Nutrient Requirements of Poultry. 9th ed. National Academy Press, Washington D.C., 1994.

S Grobas, GG Mateos and J Méndez. Influence of dietary linoleic acid on production and weight of eggs and egg components in young brown hens. J. Appl. Poult. Res. 1999; 8, 177-84.

CR Bhonzack, RH Harns, VD Merkel and GB Russell. Performance of commercial layers when fed diets with levels of corn oil or poultry fat. J. Appl. Poult. Res. 2002; 11, 68-76.

DL Fletcher, WM Britton and JA Cason. A comparison of various procedures for determining total yolk lipid content. Poult. Sci. 1984; 63, 1759-63.

AOAC. Official Methods of Analysis. 16th ed. Assoc. Off. Ana. Chem. Intl, Arlington, Virginia, 1995.

RC Damon and WR Harvey. Experimental Design, ANOVA, and Regression. Harper and Row, Publishers, New York, 1987.

K Hinkelmann and OKemptorne. Design and Analysis of Experiments, Vol. I Introduction to Experimental Design. 2nd ed. Wiley-Interscience, New Jersey, 2008.

DH Nash, RMG Hamilton and HW Hulan. The effect of dietary herring meal on the omega-3 fatty acid content of plasma and egg yolk lipids of laying hens. Can. J. Anim. Sci. 1995; 75, 247-53.

H Aida, M Hammadzic, A Gagic, M Mihajevic, J Krnic, M Vegara, M Baltic, S Trajkovic, M Kadric and EJ Pasic. Egg yolk lipid modifications by fat supplemented diets of laying hens. Acta Vet. 2005; 55, 41-51.

MR Mazalli, DE Faria, D Salvador and DT Ito. A comparison of the feeding value of different sources of fat for laying hens: 2. lipid, cholesterol, and vitamin E profile of egg yolk. J. Appl. Poult. Res. 2004; 13, 280-90.

AOCS. Physical and chemical characteristics of oils, fats and waxes. In: Official Methods and Recommended Practices of the American Oil Chemist’s Society. Section I. M197, 1997, p. 35-45.

R da Silva Filardi, OM Junqueira, AC de Laurentiz, EM Casartelli, E Aparecida Rodrigues and L Francelino Araujo. Influence of different fat sources on the performance, egg quality, and lipid profile of egg yolks of commercial layers in the second laying cycle. J. Appl. Poult. Res. 2005; 14, 258-64.

CO Leskanich and RC Noble. Manipulation of the n-3 polysaturated fatty acid composition of avian eggs and meat. World’s Poult. Sci. J. 1997; 53, 155-83.

A Meluzzi, N Tallarico, F Sirri and A Franchini. Changes in the acidic profile of the eggs as a response to the dietary administration of refined fish oils. In: Proceedings of ASPA National Congress. 1997, p. 347-8.

SJ Hosseini-Vashan, N Afzali, A Golian, M Mlekaneh and A Allahressani. Modified egg fatty acid content by supplementation of laying hen diets with palm olein oil (POO). In: Proceedings of the British Society of Animal Science. 2009, p. 212.

S Akarapunyavit. 1999, Effects of Various Fat Sources Supplemented in Laying Hen Rations, Master Thesis. Kasetsart University, Bangkok, Thailand.

SJ Hosseini-Vashan and N Afzali. Effect of different levels of palm olein oil in laying hen’s performance and yolk cholesterol. Intern. J. Poult. Sci. 2008; 7, 908-12.

WM Aymond and ME Van-Elswyk. Yolk thiobarbituric acid reactive substrates and n3 fatty acids in response to whole and ground flaxseed. Poult. Sci. 1995; 74, 1388-94.

MR Fokkerma, DAJ Brouwer, MB Hasperhoven, IA Martini and FAJ Muskiet. Short-term supplementation of low dose g-linolenic acid (GLA), a-linolenic acid (ALA), or GLA plus ALA does not augment LCPo3 status of Dutch vegans to an appreciable extent. Prostaglandins Leukot. Essent. Fatty Acids. 2000; 63, 287-92.

S Grobas, J Méndez, R Lázaro, C de Blas and GG Mateos. Influence of source and percentage of fat added to diet on performance and fatty acid composition of egg yolks of two strains of laying hens. Poult. Sci. 2001; 80, 1171-9.




How to Cite

MUANGKEOW, N. (2011). Effect of Feeding Soybean and Palm Blended Oil on Laying Performance and Egg Quality. Walailak Journal of Science and Technology (WJST), 8(1), 51–61. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/11



Research Article