A Low-Cost Analytical Method for the Morris Water Maze Task using ImageJ


  • Apsorn SATTAYAKHOM School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Phanit KOOMHIN School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand




Morris water maze task, ImageJ, Low-cost analysis method, Learning and memory impairment


The Morris water maze, which has been used for more than 30 years, is one of the most famous learning and memory tasks among animals. This method is robust and reliable, and it can be carried out anywhere with little effort. Nowadays, there are many companies creating analysis software to easily analyze the Morris water maze results. However, these softwares are costly and may be difficult for researchers in developing countries especially with limited research funds. Only escape latency and time spent in the target quadrant can be manually obtained after video review. To resolve this problem and to extend the ability to detect learning and memory impairment in the Morris water maze, we provide a novel low-cost analysis method using common office tools combined with ImageJ software to analyze learning and memory impairment in rat or mouse models. In this study, we used the 2-vessel occlusion (2VO) rats and sham-operated control rats as learning and memory deficit rats and normal rats, respectively. Taken together, travel distance, swimming speed, and swimming traces can be acquired from this method, which will help researchers to investigate further impairment of learning and memory in animal models.


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LD Hoz, SJ Martini and RGM Morris. Forgetting, reminding, and remembering: The retrieval of lost spatial memory. PloS Biol. 2004; 2, 1233-42.

P Koomhin, A Sattayakhom, S Palipoch, C Punsawad and S Tapechum. Effects of non-spatial pre- training on learning and memory impairment detection in the Morris water maze. Walailak J. Sci. & Tech. 2018; 15, 169-77.

R D'hooge and PP De Deyn. Applications of the Morris water maze in the study of learning and memory. Brain Res. Rev. 2001; 36, 60-90.

R Morris. Developments of a water-maze procedure for studying spatial learning in the rat. J. Neurosci. Meth. 1984; 11, 47-60.

CA Schneider, WS Rasband and KW Eliceiri. NIH Image to ImageJ: 25 years of image analysis. Nat. Meth. 2012; 9, 671-5.

P Koomhin, K Tilokskulchai and S Tapechum. Ceftriaxone improves spatial learning and memory in chronic cerebral hypoperfused rats. Sci. Asia 2012; 38, 356-63.

E Farkas, PGM Luiten and F Bari. Permanent, bilateral common carotid artery occlusion in the rat: A model for chronic cerebral hypoperfusion-related neurodegenerative diseases. Brain Res. Rev. 2007; 54, 162-80.

S Dalm, J Grootendorst, ERD Kloet and MS Oitzl. Quantification of swim patterns in the Morris water maze. Behav. Res. Meth. Instrum. Comput. 2000; 32, 134-9.




How to Cite

SATTAYAKHOM, A. ., & KOOMHIN, P. . (2020). A Low-Cost Analytical Method for the Morris Water Maze Task using ImageJ. Walailak Journal of Science and Technology (WJST), 17(11), 1194–1199. https://doi.org/10.48048/wjst.2020.10728