Effect of Dietary Protein Level during Estrus Cycle: Nutrient Digestibility, Quality of Embryo and Pregnancy Rate by Embryo Transfer in Thai Crossbred Goats during Hot Season


  • Juggrid JUGSUMRIT Nakhonratchasima A.I and Biotechnology Research Center, Nakhon Ratchasima 30130, Thailand
  • Chalermpon YUANGKLANG Rajamagala University of Technology Isan, Nakhon Ratchasima Campus, Nakhon Ratchasima 30000, Thailand https://orcid.org/0000-0002-3844-1691
  • Kraisit VASUPEN Rajamagala University of Technology Isan, Nakhon Ratchasima Campus, Nakhon Ratchasima 30000, Thailand
  • Benya SAENMAHAYAK Rajamagala University of Technology Isan, Nakhon Ratchasima CampusRajamagala University of Technology Isan, Nakhon Ratchasima Campus, Nakhon Ratchasima 30000, Thailand
  • Chantira WONGNEN School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80160, Thailand https://orcid.org/0000-0001-7160-8660




Embryo, Goats, Nutrition, Pregnancy rate, Reproduction, Superovulation


Tis study aimed to evaluate the embryo quality and embryo transfer rate of Thai crossbred goats fed different levels of protein in total mixed rations (TMR) during the hot season. Twenty-four non- pregnant Thai crossbred goats with an average body weight of 26±5.9 kg were assigned with a random complete block design (RCBD). Dietary treatments contained 8, 13, and 18 % crude protein (CP) in TMR. The feeding trial lasted for 42 days. Does (female goat) were scheduled to determine embryo quality by surgical laparotomy and flushing. At the end of the feeding trial, the digestion trial was conducted by the total collection method for a 5-day period. The results showed that the average total feed intake and feed conversion ratio did not differ among all treatments (p ˃ 0.05), however, digestible protein intake had increased from 70.39, 79.77 to 89.11 g/d in 8, 13, and 18 % CP group, respectively. Increasing CP levels in TMR resulted in linearly increased goat final body weight (30.25, 28.75, and 32.00 kg), weight gain (3.00, 4.00, and 4.25 kg), and average daily gain (ADG) (87.84, 114.87 and 121.63 g/d). Embryo quality (1, 1, and 3.5 morular follicle state) and pregnancy of embryo transfer rate (25, 25, and 50 %) of the animal received CP level at 8 and 13 % less than that 18 %. These results indicated that dietary protein level during program superovulation regimen affected the good quality of an embryo and the pregnancy rate in an embryo transfer in Thai crossbred goats.


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FG Gooki, M Mohammadabadi, MA Fozi and M Soflaei. Association of biometric traits with growth hormone gene diversity in Raini Cashmere Goats. Walailak J. Sci. Tech. 2019; 16, 499-508.

M Khorshidi, MR Mohammadabadi, AK Esmailizadeh, A Barazandeh and OI Babenko. Comparison of artificial neural network and regression models for prediction of body weight in Raini Cashmere goat. Iran. J. Appl. Anim. Sci. 2019; 9, 453-61.

FG Gooki, MR Mohammadabadi and MA Fozi. Polymorphism of the growth hormone gene and its effect on production and reproduction traits in goat. Iran. J. Appl. Anim. Sci. 2018; 8, 653-9.

S Shamsalddini, MR Mohammadabadi and AK Esmailizadeh. Polymorphism of the Prolactin gene and its effect on fiber traits in goat. Russ. J. Genet. 2016; 52, 405-8.

A Baghizadeh, M Bahaaddini, MR Mohamadabadi and N Askari. Allelic variations in exon 2 of Caprine MHC class II DRB3 gene in Raeini Cashmere goat. Am. J. Agri. Environ. Sci. 2009; 6, 454-9.

A Barazandeh, SM Moghbeli, M Vatankhah and MR Mohammadabadi. Estimating non-genetic and genetic parameters of pre-weaning growth traits in Raini Cashmere goat. Trop. Anim. Health Prod. 2012; 44, 811-7.

M Moghadaszadeh, MR Mohammadabadi and AK Esmailizadeh. Association of exon 2 of BMP15 gene with the litter size in the Raini Cashmere goat. Genetics in the 3rd Millenn 2015; 13, 4062-7.

MR Mohammadabadi and F Tohidinejad. Charachteristics determination of Rheb gene and protein in Raini Cashmere goat. Iran. J. Appl. Anim. Sci. 2017; 7, 289-95.

AVN Esmaeili, AE Koshkooieh, AM Aytolahi, MR Mohammadabadi, OI Babenko, M Bushtruk, S Tkachenko, R Stavetska and N Klopenko. Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran. Malays. Appl. Biol. 2019; 48, 85-93.

SM Moghbeli, A Barazandeh, M Vatankhah and MR Mohammadabadi. Genetics and non-genetics parameters of body weight for post-weaning traits in Raini Cashmere goats. Trop. Anim. Health Prod. 2013; 45, 1519-24.

MR Nassiry, FE Shahroodi, J Mosafer, A Mohammadi, E Manshad, S Ghazanfari, MR Mohammad Abadi and GE Sulimova. Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle. Russ. J. Genet. 2005; 41, 664-8.

A Norouzy, MR Nassiry, FE Shahrody, A Javadmanesh, MRM Abadi and GE Sulimova. Identification of bovine leucocyte adhesion deficiency (BLAD) carriers in Holstein and Brown Swiss AI bulls in Iran. Russ. J. Genet. 2005; 41, 1409-13.

MN Ruzina, TA Shtyfurko, MR Mohammadabadi, OB Gendzhieva, T Tsedev and GE Sulimova. Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds. Russ. J. Genet. 2010; 46, 456-63.

Department of Livestock Development. Goat farming statistic in Thailand since 1998 to 2009, Available at: http://www.dld.go.th/ict/stat_web/yearly/yearly 52, accessed January 2019.

S Miki. Effects of heat stress on bovine preimplantation embryos produced in vitro. Reprod. Dev. 2017; 63, 347-52.

PL Senger. Review: Fertility factors in high producing dairy cows-which ones are really important. Prof. Anim. Sci. 2001; 17, 129-38.

PF Surai. Vitamin E in avian reproduction. Poult. Avain. Biol. Rev. 1999; 10, 1-60.

T Blanchard, J Ferguson, L Love, T Takeda, B Henderson, J Hasler and W Chalupa. Effect of dietary crude protein type. Am. J. Vet. Res. 1990; 51, 905-8.

RD Smit and LE Chase. Nutrition and reproduction. Dairy Integrated Reproductive Management, 2010.

R Funston. Nutrition and reproduction interaction. In: Proceedings of the Applied Reproductive Strategies in Beef Cattle. Billings, Montana, 2007, p. 149-62.

LC Kappel and S Zidenberg. Manganese: Present Knowledge in nutrition. In: ML Brown (Ed.). International Life Sciences Institute Nutrition Foundation, Washington DC, 1999, p. 308.

TG Dunn and GE Moss. Effects of nutrient deficiencies and excesses on reproductive efficiency of Livestock. J. Anim. Sci. 1992; 70, 1580-93.

AOAC. Association of Official Analytical Chemists International (AOAC). Official Methods of Analysis. 17th eds. AOAC International, Gaithersburg, 2000.

ANMA Rahman, RB Abdullah and WE Wan-Khadijah. In vitro maturation of oocytes with special reference to goat: A review. Biotech. 2008; 7, 559-611.

SG Haddad. Effect of dietary forage: Concentrate ratio on growth performance and carcass characteristic of growing Baladi kids. Small Rumin. Res. 2005; 57, 43-9.

S Hwangbo, SH Choi, SH Kim, SW Son, DS Park, HS Lee and IH Jo. Effects of crude protein level in total mixed rations on growth performance and meat quality in growing Korean black goats. Asian Aust. J. Anim. Sci. 2009; 22, 1133-9.

I Prieto, AL Goetsh, V Banskalieva, M Cameron, R Puchala, T Sahlu, LJ Dawson and SW Coleman. Effects of dietary protein concentration on postweaning growth of Boer crossbred and Spanish goat wethers. J. Anim. Sci. 2002; 78, 2275-81.

T Negesse, M Rodehutscord and E Pfeffer. The effect of dietary crude protein level on intake growth protein retention and utilization of growing male Saanen kids. Small Rumin. Res. 2001; 39, 243-51.

W Pralomkarn, S Saithanoo, S Kochapakdee and BW Norton. Effect of genotype and plane of nutrition on carcass characteristics of Thai native and Anglo-Nubian X Thai native male goats. Small Rumin. Res. 1995; 16, 21-5.

K Intharak and J Saelim. Effect of crude protein concentration in total mixed rations on growth performance of Ango-Nubian crossbred goats. Annual Research Report 2008, Animal Nutrition Division, Department of Livestock Development, Ministry of Agriculture and Cooperative, p. 246-70.

F Kanyinji, H Kumagai, T Maeda, S Kaneshima and D Yokoi. Effects of supplementary inosine on nutrient digestibility ruminal fermentation and nitrogen balance in goats fed high amount of concentrate. Anim. Feed Sci. Tech. 2009; 152, 12-20.

AK Pathak. Various factors affecting microbial protein synthesis in the rumen. Vet. World. 2008; 1, 186-9.

A Bach, S Calsamiglia and MD Stern. Nitrogen metabolism in the rumen. J. Dairy Sci. 2005; 88, 9-21.

R Webb, B Nicholas, JG Gong, BK Campbell, CG Gutierrez, HA Garverick and DG Armstrong. Mechanisms regulating follicular development and selection of the dominant follicle. Reprod. Suppl. 2003; 61, 1-20.

D Rondian, VJF Freitas, M Spinaci and G Galeati. Effect of nutrition on plasma progesterone levels metabolic parameters and small follicles development in unstimulated goats reared under constant photoperiod regimen. Reprod. Domest. Anim. 2005; 40, 548-52.

JA Downing and RJ Scaramuzzi. Nutrient effects on ovulation rate ovarian function and the secretion of gonadotrophic and metabolic hormones in sheep. J. Reprod. Fertil. 1991; 43, 209-27.

R Funston. Nutrition and Reproduction Interaction, University of Nebraska, West Central Research and Extension Center, North Platte. In: Proceedings of the Applied Reproductive Strategies in Beef Cattle, Rapid City, South Dakota, 2006.

RJV Saun. Effect of nutrition on reproduction in llamas and alpacas. Theriogenology 2008; 70, 508-14.

J Fahey, MP Boland and DO Callaghan. Effects of dietary urea on embryo development in super ovulated donor ewes and on embryo survival following transfer in recipient ewes. Proc. Annual Meet. British Soc. Animal Sci. 1998; 1998, 182.

WR Butler. Review: Effect of protein nutrition on ovarian and uterine physiology in dairy cattle. J. Dairy Sci. 1999; 81, 2533-9.




How to Cite

JUGSUMRIT, J. ., YUANGKLANG, C. ., VASUPEN, K. ., SAENMAHAYAK, B. ., & WONGNEN, C. . (2021). Effect of Dietary Protein Level during Estrus Cycle: Nutrient Digestibility, Quality of Embryo and Pregnancy Rate by Embryo Transfer in Thai Crossbred Goats during Hot Season. Walailak Journal of Science and Technology (WJST), 18(4), Article 10522 (10 pages). https://doi.org/10.48048/wjst.2021.10522