TY - JOUR AU - ABDISSA, Dele AU - FAYE, Gezahegn AU - FAYERA, Melkamu AU - DEMISSIE, Shiferaw PY - 2020/07/01 Y2 - 2024/03/29 TI - Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride JF - Walailak Journal of Science and Technology (WJST) JA - Walailak J Sci & Tech VL - 17 IS - 7 SE - Research Article DO - 10.48048/wjst.2020.6163 UR - https://wjst.wu.ac.th/index.php/wjst/article/view/6163 SP - 631-638 AB - <p><em>Kniphofia pumila </em>has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of <em>K. pumila </em>and perform<em> in vitro </em>antibacterial activity tests against selected pathogens. Extraction of compound from<strong> t</strong>he roots of <em>K. pumila </em>was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl<sub>2</sub> were tested against pathogenic bacteria <em>Escherichia</em><em> coli </em>(ATCC 25722)<em>, </em><em>Klebsiella</em> <em>pneumoniae</em> subsp. <em>pneumoniae</em> (DSM 19613), <em>Staphylococcus </em><em>aureus</em> (ATCC 25925) and <em>Salmonella Typhimurium </em>(ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-<strong>1</strong>) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl<sub>2</sub> were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as <sup>1</sup>H-NMR, <sup>13</sup>C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-<strong>1 </strong>was isolated from the roots of <em>K. pumila </em>and it was noted that the antibacterial activities of ZnCl<sub>2</sub> in combination with crude extract as well as with isolated compound against <em>E. coli </em>(ATCC 25722),<em> S. aureus </em>(ATCC 25925) and <em>S. Typhimurium</em> (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of <em>K. pumila </em>one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl<sub>2</sub> salt and alone.</p> ER -