Synergism of Zinc Oxide Nano-Powder with Active Compound from Turmeric and Lemongrass as Bacterial Inhibitor

Gee Een  LAU; Che Azurahanim  CHE ABDULLAH; Mohd Sabri  MOHD GHAZALI; Muhamad Syazlie  CHE IBRAHIM

doi:10.48048/wjst.2021.9652

Authors

Gee Een LAU Material Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Selangor, Malaysia
Che Azurahanim CHE ABDULLAH Department of Physics, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
Mohd Sabri MOHD GHAZALI Department of Nano Physics, Universiti Malaysia Terengganu, Terenggganu, Malaysia
Muhamad Syazlie CHE IBRAHIM Department of Biological Science, Universiti Malaysia Terengganu, Terengganu, Malaysia

DOI:

https://doi.org/10.48048/wjst.2021.9652

Keywords:

Metal oxide, Green synergism, Escherichia coli, Synergism, Green product, Active Compound, Turmeric, Lemongrass, Bacterial inhibitor

Abstract

Green nanoparticles are receiving great attention due to their broad fields of application. Plant extracts are promising sources, since the synergizing process is easy and cost-effective. Green synergism offers safer nanomaterials for both human health and the environment. The antimicrobial application of prepared nanoparticles is due to special capabilities at the nanoscale size. Green zinc oxide nanoparticles (ZnONPs) from lemongrass and turmeric revealed good antibacterial properties. Antibacterial active compounds were extracted from lemongrass and turmeric using methanol as a solvent. The extracts were introduced to zinc oxide solution to create synergized green ZnONPs and tested for their antibacterial properties. The samples were characterized by means of XRD, SEM, FTIR, and GC-MS. The zones of inhibition of synergized green ZnONPs were successfully measured using the disc diffusion method. The findings using gram-negative bacteria Escherichia coli. showed a higher antibacterial inhibition zone, with a diameter of 1.6 cm. The gram-positive bacteria, Staphylococcus aureus., showed low antibacterial inhibition. The addition of ZnO had positively revealed greater inhibition. Green ZnONPs synthesized using plant extracts will be further tested for various applications.

HIGHLIGHTS

Green zinc oxide nanoparticles (ZnONPs) prepared from lemongrass and turmerics
Plant extracts are significant in reducing and stabilizing metallic ions
The synergism of ZnONPs was successfully prepared from plant extract active and evaluated for their antibacterial activity using Staphylococcus aureus and Escherichia coli
The zones of inhibition of synergized green ZnONPs measured using the disc diffusion method showed a higher antibacterial inhibition zone
Antibacterial activity revealed the potential applications of green synergism of ZnONPs for treatment of contaminated water, medical applications, and cosmetics

GRAPHICAL ABSTRACT

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References

S Sarkar and SC Sarkar. Chapter-4 application of nanotechnology in medicine. Med. Sci. 2019; 117, 49.

S Ali, S Rasheed, I Hassan and S Rashid. Role of nanotechnology in medicine and cancer treatment. In: Proceedings of the 5th International Conference on Nanptechnology for Better Living, SKUAST, Shalimar Srinagar, Jammu and Kashmir, India, 2019, p. 311-2.

AP Nikalje. Nanotechnology and its applications in medicine. Med. Chem. 2015; 5, 81-9.

Y Liu, L Shi, L Su, HCVD Mei, PC Jutte, Y Ren and HJ Busscher. Nanotechnology-based antimicrobials and delivery systems for biofilm-infection control. Chem. Soc. Rev. 2019; 48, 428-46.

PV Sekar, VD Parvathi and R Sumitha. Green nanotechnology in cadmium sulphide nanoparticles and understanding its toxicity and antimicrobial properties. Biomed. Res. 2019; 30, 805-9.

P Li, J Li, C Wu, Q Wu and J Li. Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology 2005; 16, 1912.

AE Allen and DWC Macmillan. Synergistic catalysis: A powerful synthetic strategy for new reaction development. Chem. Sci. 2012; 3, 633-58.

J Jiang, J Pi and J Cai. The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorg. Chem. Appl. 2018; 2018, 1062562.

K Ranoszek-Soliwoda, E Tomaszewska, K Małek, G Celichowski, P Orlowski, M Krzyzowska and J Grobelny. The synthesis of monodisperse silver nanoparticles with plant extracts. Colloids Surf. B Biointerfaces 2019; 177, 19-24.

S Iravani, H Korbekandi, SV Mirmohammadi and B Zolfaghari. Synthesis of silver nanoparticles: Chemical, physical and biological methods. Res. Pharm. Sci. 2014; 9, 385-406.

MI Naik, BA Fomda, E Jaykumar and JA Bhat. Antibacterial activity of lemongrass (Cymbopogon citratus) oil against some selected pathogenic bacterias. Asian Pac. J. Trop. Med. 2010; 3, 535-8.

Bhawana, RK Basniwal, HS Buttar, VK Jain and N Jain. Curcumin nanoparticles: Preparation, characterization, and antimicrobial study. J. Agric. Food Chem. 2011; 59, 2056-61.

H Mirzaei and M Darroudi. Zinc oxide nanoparticles: Biological synthesis and biomedical applications. Ceram. Int. 2017; 43, 907-14.

SZ Moghadamtousi, HA Kadir, P Hassandarvish, H Tajik, S Abubakar and K Zandi. A review on antibacterial, antiviral, and antifungal activity of curcumin. Biomed. Res. Int. 2014; 2014, 186864.

A Azam, AS Ahmed, M Oves, MS Khan, SS Habib and A Memic. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: A comparative study. Int. J. Nanomedicine 2012; 7, 6003-9.

K Das, RKS Tiwari and DK Shrivastava. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. J. Med. Plants Res. 2010; 4, 104-11.

M Shah, D Fawcett, S Sharma, SK Tripathy and GEJ Poinern. Green synthesis of metallic nanoparticles via biological entities. Materials (Basel) 2015; 8, 7278-308.

NE Tajidin, SH Ahmad, AB Rosenani, H Azimah and M Munirah. Chemical composition and citral content in lemongrass (Cymbopogon citratus) essential oil at three maturity stages. Afr. J. Biotechnol. 2012; 11, 2685-93.

AA Alafiatayo, S Ahmad and M Maziah. Total antioxidant capacity, total phenolic compounds and the effects of solvent concentration on flavonoid content in Curcuma longa and Curcuma xanthorhhiza rhizomes. Med. Aromat. Plants 2014; 3, 1-4.

B Balakrishnan, S Paramasivam and A Arulkumar. Evaluation of the lemongrass plant (Cymbopogon citratus) extracted in different solvents for antioxidant and antibacterial activity against human pathogens. Asian Pac. J. Trop. Dis. 2014; 4, S134-S139.

SO Ogunyemi, Y Abdallah, M Zhang, H fouad, X Hong, E Ibrahim, MMI Masum, A Hossain, J Mo and B Li. Green synthesis of zinc oxide nanoparticles using different plant extracts and their antibacterial activity against Xanthomonas oryzae pv. oryzae. Artif. Cells Nanomed. Biotechnol. 2019; 47, 341-52.

AM Awwad, MW Amer, NM Salem and AO Abdeen. Green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Ailanthus altissima fruit extracts and antibacterial activity. Chem. Int. 2020; 6, 151-9.

Y Fukuyama, N Yasuda, K Sugimoto and S Kimura. X-ray diffraction measurement of a single nanometre-sized particle levitated in air by an optical-trap sample holder. J. Synchrotron Radiat. 2020; 27, 67-74.

Y Gao, MAV Anand, V Ramachandran, V Karthikkumar, V Shalini, S Vijayalakshmi and D Ernest. Biofabrication of zinc oxide nanoparticles from aspergillus niger, their antioxidant, antimicrobial and anticancer activity. J. Clust. Sci. 2019; 30, 937-46.

OJ Nava, PA Luque, CM Gómez-Gutiérrez, AR Vilchis-Nestor, A Castro-Beltran, ML Mota-Gonzalez and A Olivas. Influence of Camellia sinensis extract on zinc oxide nanoparticle green synthesis. J. Mol. Struct. 201; 1134, 121-5.

R Waseem and KH Low. Advanced analytical techniques for the extraction and characterization of plant-derived essential oils by gas chromatography with mass spectrometry. J. Sep. Sci. 2015; 38, 483-501.

ND Bharadwaj and AK Sharma. Detection of escherichia coli, staphylococcus aureus and salmonella typhi in drinking water of government institutions and organizations of gwalior city. Int. J. Eng. Sci. Res. Technol. 2016; 5, 769-74.

A Altemimi, N Lakhssassi, A Baharlouei, DG Watson and DA Linghtfoot. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants (Basel) 2017; 6, 42.

S Vijayakumar, K Saravanakumar, B Malaikozhundan, M Divya, B Vaseeharan, EF Duran-Lara and MH Wang. Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles for anti MRSA therapy. Int. J. Biol. Macromol. 2020; 144, 9-18.

AK Zak, WHA Majid, ME Abrishami and R Yousefi. X-ray analysis of ZnO nanoparticles by Williamson-Hall and size-strain plot methods. Solid State Sci. 2011; 13, 251-6.

NI Rasli, H Basri and Z Harun. Zinc oxide from aloe vera extract: Two-level factorial screening of biosynthesis parameters. Heliyon 2020; 6, e03156.

TS Anvekar, VR Chari and H Kadam. Green synthesis of ZnO nanoparticles, its characterization and application. Mat. Sci. Res. India 2017; 14, 153-7.

RI Uchegbu, LC Ngozi-Olehi and RU Ogbuneke. Essential oils composition of Curcuma longa rhizome from Nigeria. Am. J. Chem. Appl. 2014; 1, 1-5.

BK Paul, MMU Munshi, MN Ahmed, GC Saha and SK Roy. The fatty acid composition and properties of oil extracted from fresh rhizomes of turmeric (Curcuma longa Linn.) cultivars of Bangladesh. Bangladesh J. Sci. Ind. Res. 2011; 46, 127-32.

LA Araujo, RGM Araujo, FO Gomes, SR Lemes, LM Almeida, LJQ Maia, PJ Gonçalves, F Mrué, NJ Silva-Junior and PRDE Melo-Reis. Physicochemical/photophysical characterization and angiogenic properties of Curcuma longa essential oil. An. Acad. Bras. Cienc. 2016; 88, 1889-97.

M Jayandran and M Haneefa. Green synthesis and antimicrobial activity studies of Curcuminaline functionalized zinc oxide nanoparticles and comparative studies with its non-functionalized form. Int. J. Pharm. Sci. Res. 2016; 7, 4117-24.

B Kumar, V Singh, R Shankar, K Kumar and RK Rawal. Synthetic and medicinal prospective of structurally modified curcumins. Curr. Top. Med. Chem. 2017; 17, 148-61.

RN Moussawi and D Patra. Nanoparticle self-assembled grain like curcumin conjugated ZnO: Curcumin conjugation enhances removal of perylene, fluoranthene, and chrysene by ZnO. Sci. Rep. 2016; 6, 24565.

AS Elfeky, SS Salem, AS Elzaref, ME Owda, HA Eladawy, AM Saeed, MA Awad, RE Abou-Zeid and A Fouda. Multifunctional cellulose nanocrystal/metal oxide hybrid, photo-degradation, antibacterial and larvicidal activities. Carbohydr. Polym. 2020; 230, 115711.

P Negi, M Aggarwal, G Sharma, C Rathore, G Sharma, B Singh and OP Katare. Niosome-based hydrogel of resveratrol for topical applications: An effective therapy for pain related disorder(s). Biomed. Pharmacother. 2017; 88, 480-7.

S Chirumbolo. The role of quercetin, flavonols and flavones in modulating inflammatory cell function. Inflamm. Allergy Drug Targets 2010; 9, 263-85.

JM Al-Shuneigat, SA Al-Sarayreh, MA Al-Qudah and YM Al-Saraireh. Antibacterial and antibiofilm activity of essential oil of Achillea biebersteinii and its mode of action. J. Pharm. Pharmacogn. Res. 2020; 8, 155-66.

M Jayandran, M. Haneefa and V Balasubramanian. Biosynthesis and antimicrobial activity studies of salicylalchitosan functionalized zinc oxide nanoparticles and comparative studies with its non-functionalized form. Orient. J. Chem. 2016; 32, 719-25.

K Jemal, BV Sandeep and S Pola. Synthesis, characterization, and evaluation of the antibacterial activity of Allophylus serratus leaf and leaf derived callus extracts mediated silver nanoparticles. J. Nanomater. 2017; 2017, 1-11.

YN Slavin, J Asnis, UO Häfeli and H Bach. Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J. Nanobiotechnol. 2017; 15, 65.