Methemoglobin and Sulfhemoglobin Levels in Students of Walailak University

Authors

  • Uthai TRIAPIRUX School of Allied Health Science and Public Health, Walailak University, Nakhon Si Thammarat 80161
  • Prasit NA-EK School of Allied Health Science and Public Health, Walailak University, Nakhon Si Thammarat 80161
  • Chinnaphat KITTHIPONGVIVAT School of Allied Health Science and Public Health, Walailak University, Nakhon Si Thammarat 80161
  • Surachate CHALAJIT School of Allied Health Science and Public Health, Walailak University, Nakhon Si Thammarat 80161

Keywords:

Methemoglobin (MHb), Sulfhemoglobin (SHb), Spectrophotometry

Abstract

Methemoglobin (MHb) and Sulfhemoglobin (SHb) are abnormal oxygen-carrying protein hemoglobins (Hbs) in blood circulation which can be used to represent the degree of pollution in the surrounding environment including air, food and drinks. The MHb is an oxidized form of hemoglobin (Hb) in which the ferrous ion (Fe2+) in the heme group is oxidized to the ferric ion (Fe3+) by oxidizing agents mostly from the air, food, drinks and drugs. The SHb is a sulfurated form of Hb derived from hydrogen sulfide (H2S) and sulfur dioxide (SO2) gas contained in the air and drugs found in sulfur therapy, respectively. These types of Hb derivatives are unable to bind and carry oxygen to the tissues of the body. This makes inspiration insufficient and may cause cyanosis in increased amounts. The aim of this study is to measure MHb and SHb levels in Walailak University students. EDTA blood samples were collected from 200 students. The MHb and SHb were analyzed by spectrophotometry. The results revealed that the mean MHb and SHb levels of the 200 subjects were 1.29 ± 0.57 % and 0.66 ± 0.28 %, respectively. The level of MHb and SHb in population of Walailak University was very low compared to people in large cities such as Bangkok. The study proves that the environment of Walailak University remains clean, good and fresh.

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References

DG Badman and ER Jaffe. Blood and air pollution: state of knowledge and research needs. Otolaryng. Head Neck 1996; 114, 205-8.

AM Fan and VE Steinberg. Health implications of nitrate and nitrite in drinking water: an update on methemoglobinemia occurrence and reproductive and developmental toxicity. Regul. Toxicol. Pharmacol. 1996; 23, 35-43.

S Curry. Methemoglobinemia. Ann. Emerg. Med. 1982; 11, 214-21.

A Mansouri and AA Lurie. Concise review: methemoglobinemia. Am. J. Hematol. 1993; 42(1), 7-12.

FM Henretig, B Gribetz and T Kearney. Interpretation of color change in blood with varying degree of methemoglobinemia. J. Toxicol. Clin. Toxicol. 1988; 26, 293-301.

RO Wright, WJ Lewander and AD Woolf. Methemoglobinemia: etiology, pharmacology, and clinical management. Ann. Emerg. Med. 1999; 34, 646-56.

A Tangerman, G Bongaerts, R Agbeko, B Semmekrot and R Severijnen. The origin of hydrogen sulfide in a newborn with sulfhaemoglobin induced cyanosis. J. Clin. Pathol. 2002; 55, 631-3.

OK Baskurt. Acute hematologic and hemorheologic effects of sulfur dioxide inhalation. Arch. Environ. Health. 1988; 43, 344-8.

DG Martin, CL Woodard Jr, MB Gold, CE Watson and SI Baskin. Methemoglobin and sulfhemoglobin formation due to benzocaine and lidocaine in Macaques. Defense Technical Information Center. Available at: http://stinet.dtic.mil/oai/oai?verb=getRecord&metadataPrefix=html&identifier=ADP008849, accessed April 2008.

JS Harris and HO Michel. The formation of methemoglobin and sulfhemoglobin during sulfanilamide therapy. J. Clin. Invest. 1939; 18, 507-19.

KE Ward and MW McCarthy. Dapsone-induced methemoglobinemia. Ann. Pharmacother. 1998; 32, 549-53.

AS Gopalachar, VL Bowie and P Bharadwaj. Phenazopyridine-induced sulfhemoglobinemia. Ann. Pharmacother. 2005; 39, 1128-30.

PJ Van Veldhuizen and A Wyatt. Metoclopramide-induced sulfhemoglobinemia. Am. J. Gastroenterol. 1995; 90, 1010-11.

M Savic, J Siriski-Sasic and D Djulizibaric. Discomforts and laboratory findings in workers exposed to sulfur dioxide. Int. Arch. Occ. Env. Hea. 1987; 59, 513-8.

CM Park and RL Nagel. Sulfhemoglobinemia, clinical and molecular aspects. N. Engl. J. Med. 1984; 310, 1579-84.

A Tomoda, A Kakizuka and Y Yoneyama. Oxidative and reductive reactions of sulphhaemoglobin with various reagents correlated with changes in quaternary structure of the protein. Biochem. J. 1984; 221, 587-91.

MJ Percy, NV McFerran and TR Lappin. Disorders of oxidised haemoglobin. Blood Rev. 2005; 19, 61-8.

RP Smith. Chemicals reacting with various forms of hemoglobin: biological significance, mechanisms, and determination. J. Forensic Sci. 1991; 36, 662-72.

KA Everyn and HT Malloy. Microdetermination of oxyhemoglobin, methemoglobin and sulfhemoglobin in a single sample of blood. J. Biol. Chem. 1938; 126, 655-62. Available at: http://www.jbc.org/cgi/reprint/126/2/655, accessed April 2008.

VS Rai and PS Minty. The determination of carboxyhaemoglobin in the presence of sulphaemoglobin. Forensic Sci. Int. 1987; 33, 1-6.

A Zwart, EJ van Kampen and WG Zijlstra. Results of routine determination of clinically significant hemoglobin derivatives by multicomponent analysis. Clin. Chem. 1986; 32, 972-8.

FL Rodkey and JD O’Neal. Effects of carboxyhemoglobin on the determination of methemoglobin in blood. Clin. Chem. 1974; 20, 1116-20.

EJ van Kampen and WG Zijlstra. Spectrophotometry of hemoglobin and hemoglobin derivatives. Adv. Clin. Chem. 1983; 23, 199-257.

A Zwart, A Buursma, EJ van Kampen and WG Zijlstra. Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer. Clin. Chem. 1984; 30, 373-9.

AW Nichol and DB Morell. Spectrophotometric determination of mixtures of sulphaemoglobin and methaemoglobin in blood. Clin. Chim. Acta 1968; 22, 157-60.

P Dhareeruchata, S Julnimi, A Jitrbanjong, U Patanapolpaibul and P Laongpanich. Methemoglobin and sulfhemoglobin levels in healthy Thai subjects. J. Med. Tech. Assoc. Thai 1997; 25, 143-50.

M Denshaw-Burke. Methemoglobinemia from emedicine website, Available at: http://www.emedicine.com/med/TOPIC1466.HTM, accessed April 2008.

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Published

2011-11-13

How to Cite

TRIAPIRUX, U., NA-EK, P., KITTHIPONGVIVAT, C., & CHALAJIT, S. (2011). Methemoglobin and Sulfhemoglobin Levels in Students of Walailak University. Walailak Journal of Science and Technology (WJST), 5(2), 173–180. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/87

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Research Article