Germination and Regeneration of Cymbidium findlaysonianum Lindl. on a Medium Supplemented with Some Organic Sources

Supavadee TAWARO; Potjamarn SURANINPONG; Sontichai CHANPRAME

Authors

Supavadee TAWARO Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla 90110
Potjamarn SURANINPONG Division of Crop Production Technology, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161
Sontichai CHANPRAME Department of Agronomy, Faculty of Agriculture, Kasetsart University, Khamphaeng Saen Campus, Nakhon Pathom 73140

Keywords:

Cymbidium findlaysonianum, germination, organic additives, protocorm

Abstract

Seeds of Cymbidium findlaysonianum Lindl. were germinated and regenerated on media supplemented with various additives. After germination for 3 months, solidified Vacin and Went (VW) medium supplemented with, 15 % coconut water, 5 % banana homogenate, 5 % potato homogenate, 0.2 % activated charcoal and 20 g/l sucrose promoted higher seedling germination than the control medium. The number of protocorm cultured in a modified liquid VW medium with Murashige and Skoog (MS) vitamin (VWM) increased 4 times for every month of culture, a significant difference from the VW medium, MS medium and half strength MS medium (½ MS). Moreover, the activated charcoal added to the medium stimulated seedlings growth significantly more than when coconut water, banana homogenate and/or potato homogenate were added to the medium. Healthy plants transferred to a plastic tray containing coconut peat successfully acclimatized (70 %) in the greenhouse. More than 100,000 plantlets may be obtained from a capsule after being cultured for a year. Thus, organic additives and medium components had an effect on the growth and development of asymbiotic seeds in C. findlaysonianum.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

T Opchat. Orchids in Thailand. Home and Garden Publishing (in Thai), Bangkok, 2000, p. 2.

JM Van Waes and PC Debergh. In vitro germination of some Western European orchids. Physio. Plant. 1986; 67, 253-61.

L Kunduson. A nutrient for germination of orchid seeds. Am. Orchid Soc. Bull. 1946; 15, 214-7.

C Chen and WC Chen. Plant regeneration via embryo and shoot formation from flower-stalk explants of Oncidium ‘Sweet Sugar’. Plant Cell Tissue Org. Cult. 2000; 62, 95-100.

SY Park, HN Murthy and KY Paek. Protocorm-likes body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis. Plant Sci. 2003; 164, 919-23.

SF Lo, SM Nalawade, CL Kuo, CL Chen and HS Tsay. Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense Makino - a medicinally important orchid. In Vitro Cell. Dev. Bio. Plant. 2004; 40, 528-35.

V Nagaraju and SK Mani. Rapid in vitro propagation of orchid Zygopetalum intermedium. J. Plant. Biochem. Biotech. 2005; 14, 27-32.

R Kishor, PSV Khan and GJ Sharma. Hybridization and in vitro culture of an orchid hybrid Ascocenda ‘Kangla’. Sci. Hort. 2006; 108, 66-73.

RB Mitchell. Growing hardy orchids from seeds at Kew. Plantsman 1989; 11, 152-69.

J Arditti, R Ersnt, TW Yam and C Glabe. The contribution of orchid mycorrhizal fungi to seed germination: a speculative review. Lindleyana 1990; 5, 249-55.

EF Vacin and FW Went. Some pH changes in nutrient solutions. Bot. Gaz. 1949; 110, 605-13.

T Murashige and F Skoog. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962; 15, 473-97.

J Arditti and R Ernst. Physiology of Germinating Orchid Seeds. In: Arditti J (ed.). Orchid Biology: Reviews and Perspectives, Vol III. Cornell University Press, Ithaca, NY, 1984, p.176-222.

G Harvais. An improved culture medium for growing the orchid Cypripedium reginae axenically. Canadian J. Bot. 1982; 60, 2547-56.

MA De Pauw and WR Remphrey. In vitro germination of three Cypripedium species in relation to time of collection, media and cold treatment. Canadian J. Bot. 1993; 71, 879-85.

M Nath, J Devi, B Borthakur, J Sharma and PC Deka. Embryo culture of Rhynchostylis retusa and Vanda coerlea. J. Orchid Soc. Ind. 1991; 5, 79-101.

JA Teixeiva da Silva, M Chan, T Sanjaya, ML Chai and M Tanaka. Priming abiotic factors for optimal hybrid Cymbidium (Orchidaceae) PLB and callus induction, plantlet formation, and their subsequent cytogenetic stability analysis. Sci. Hort. 2006; 109, 368-78.

J Roy and N Banerjee. Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. Var. oculatum Hk. f. Sci. Hort. 2003; 97, 333-40.

GB Kerbary. Plant regeneration of Oncidium varicosum (Onchidaceae) by means of root tip culture. Plant Cell Rep. 1984; 3, 27-9.

SS Sheelavanthmath, HN Murthy, BP Hema, EJ Hahn and KY Paek. High frequency of protocorm like bodies (PLBs) induction and plant regeneration from protocorm and leaf sections of Aerides crispum. Sci. Hort. 2005; 106, 395-401.

SC van Winkle and GS Pullman. Achieving desired plant growth regulator levels in liquid plant tissue culture media that include activated carbon. Plant Cell Rep. 2005; 22, 303-11.

S Ichihashi and MO Islam. Effect of complex organic additives on callus growth in three orchid genera, Phalaenopsis, Doritaenopsis and Neofinetia. J. Jpn. Soc. Hort. Sci. 1999; 68, 269-74.