Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

  • Dele ABDISSA Department of Chemistry, College of Natural Sciences, Jimma University, Jimma, Ethiopia
  • Gezahegn FAYE Department of Chemistry, College of Natural Sciences, Salale University, Fiche, Ethiopia
  • Melkamu FAYERA Department of Chemistry, College of Natural Sciences, Jimma University, Jimma, Ethiopia
  • Shiferaw DEMISSIE Department of Biology, College of Natural Sciences, Jimma University, Jimma, Ethiopia
Keywords: Kniphofia pumila, Knipholone, Maceration method, Crude extracts, Zinc chloride

Abstract

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

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Author Biographies

Dele ABDISSA, Department of Chemistry, College of Natural Sciences, Jimma University, Jimma, Ethiopia

My Name is  Dele Abdissa. I have got my M.Sc degree in  Organic Chemistry.  And now, I am Lecturer at Jimma University Chemistry Department.

Jimma, Ethiopia

Gezahegn FAYE, Department of Chemistry, College of Natural Sciences, Salale University, Fiche, Ethiopia

My Name is Gezahegn Faye. I have got my M.Sc degree inorganic  Chemistry.  And now, I am Ass.Prof. at Selale University Chemistry Department.

Fiche, Ethiopia

Melkamu FAYERA, Department of Chemistry, College of Natural Sciences, Jimma University, Jimma, Ethiopia

My Name is  Melkamu Fayera. I have got my M.Sc degree in  Organic Chemistry.  And now, I am Lecturer at Jimma University Chemistry Department.

Jimma, Ethiopia

Shiferaw DEMISSIE, Department of Biology, College of Natural Sciences, Jimma University, Jimma, Ethiopia

My Name is  Shifera Demissie. I have got my M.Sc degree in  Microbiology.  And now, I am Ass.Prof. at Jimma University Chemistry Department.

            Jimma, Ethiopia

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Published
2020-07-01
How to Cite
ABDISSA, D., FAYE, G., FAYERA, M., & DEMISSIE, S. (2020). Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride. Walailak Journal of Science and Technology (WJST), 17(7), 631-638. Retrieved from http://wjst.wu.ac.th/index.php/wjst/article/view/6163