Biodegradation of Polyvinyl Alcohol by Thai Indigenous Mixed Microbial Culture

  • Duangkhaetita KANJANASOPA Agricultural Science and Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
  • Benchamaporn PIMPA Food Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
  • Suraphon THITITHANAKUL Agricultural Science and Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
  • Suwaluk WISUNTHORN Rubber Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
Keywords: Biodegradation, Polyvinyl alcohol, PVA degrading microorganism, Mixed microbial cultures

Abstract

PVA is biodegradable plastic and a water-soluble synthetic polymer that plays a significant role in industry. A large amount of PVA in wastewater causes heavy environmental pollution in terms of accumulation, disposal, and long-term degradation; therefore it must be removed from wastewater before the water is discharged. In this study, NS3 mixed microbial culture, capable of completely degrading 5 g.L-1 polyvinyl alcohol (PVA), was isolated from landfill soil using the enrichment culture method. It completely degraded PVA at an initial concentration in the range 1 - 5 g.L-1 over 5 - 20 days of incubation with continuous shaking at 30 °C. Moreover, mixed microbial cultures were found to remove PVA at a high range concentration of 10 - 25 g.L-1. Urea and glucose added to the medium inhibited PVA degradation by increasing the pH to a strongly alkaline level, which would cause cell viability and enzyme stability. The FT-IR spectra and SEM imaging revealed the mechanisms and the physical degradation of PVA films, respectively. PVA uptake in bacterial cells produced a dent in the cell surface, which represented the consumption of PVA by bacterial cell. The PVA-degrading mixed microbial culture is the first reported in Thailand and can be beneficial in PVA wastewater treatment.

Downloads

Download data is not yet available.

References

M Shimao. Biodegradation of plastics. Curr. Opin. Biotechnol. 2001; 2, 242-7.

K Sakai, M Fukuba, Y Hasui, K Moriyoshi, T Ohmoto, T Fujita and T Ohe. Purification and characterization of an esterase involved in poly (vinyl alcohol) degradation by Pseudomonas vesicularis PD. Biosci. Biotechnol. Biochem.1998; 62, 2000-7.

S Mutsumura, N Tomizawa, A Toki, K Nishikawa and K Toshima. Effect of molecular-weight and stereo-regularity on degradation of poly (vinyl alcohol) by Alcaligenes faecalis. Biotechnol. Lett. 1994; 16, 1205-10.

A Yamatsu, R Matsumi, H Atomi and T Imanaka. Isolation and characterization of a novel poly (vinyl alcohol)-degrading bacterium”, Sphingopyxis sp. PVA3. Appl. Microbiol. Biotechnol. 2006; 72, 804-11.

M Shimao, K Ninomiya, O Kuno, N Kato and C Sakazawa. Existence of novel enzyme, pyrroloquinoline quinine-dependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, Pseudomonas sp. strain VM15C. Appl. Environ. Microbiol. 1986; 5, 268-75.

T Mori, M Sakimoto, T Kagi and T Sakai. Isolation and characterization of a strain of Bacillus megaterium that degrades poly (vinyl alcohol). Biosci. Biotechnol. Biochem.1996; 60, 330-2.

BC Kim, CK Sohn, SK Lim, JW Lee and W Park. Degradation of polyvinyl alcohol by Sphingomonas sp. SA3 and its symbiote. J. Ind. Microbiol. Biotechnol. 2003; 30, 70-4.

JA Lee and MN Kim. Isolation of new and potent poly (vinyl alcohol)-degrading strains and their degradation activity. Polym. Degradation Stab. 2003; 81, 303-8.

HZ Zhang. Influence of pH and C/N ratio on poly (vinyl alcohol) biodegradation in mixed bacterial culture. J. Polym. Environ. 2009; 17, 286-90.

B Tang, X Liao, D Zhang, M Li, R Li, K Yun, G Du and J Chen. Enhanced production of poly (vinyl alcohol)-degrading enzymes by mixed microbial culture using 1,4-butanediol and designed fermentation strategies. Polym. Degradation Stab. 2010; 95, 557-63.

F Kawai and XP Hu. Biochemistry of microbial polyvinyl alcohol degradation. Appl. Microbial. Biotechnol. 2009; 84, 227-37.

J Chen, Y Zhang, GC Du, ZZ Hau and Y Zhu. Biodegradation of polyvinyl alcohol by a mixed microbial culture. Enzyme Microb. Tech. 2007; 40, 1686-91.

JH Finley. Spectrophotometric determination of polyvinyl alcohol in paper coatings. Anal. Chem. 1961; 33, 1925-7.

T Vaclavkova, J Ruzicka, M Julinova, R Vicha and M Koutny. Novel aspects of symbiotic (polyvinyl alcohol) biodegradation. Appl. Microbiol. Biotechnol. 2007; 76, 911-7.

D Qian, G Du and J Chen. Isolation and culture characterization of a new polyvinyl alcohol-degrading strain: Penicillium sp. WSH02-21. World J. Microbe. Biot. 2004; 20, 587-91.

J Huang, S Yang and S Zhang. Performance and diversity of polyvinyl alcohol-degrading bacteria under aerobic and anaerobic conditions. Biotechnol. Lett. 2016; 38, 1875-80.

Q Yang, W Zhang, H Zhang, Y Li and C Li. Wastewater treatment by alkali bacteria and dynamics of microbial communities in two bioreactors. Bioresour. Tech. 2011; 102, 3790-8.

D Cetin and S Citak. Degradation of polyvinyl alcohol by a mixed microbial culture isolated from paper mill treatment. Gazi Univ. J. Sci. 2014; 27, 839-45.

ZY Hu, GC Du, ZZ Hua and J Chen. Effect of different carbon and nitrogen sources on PVA degradation in a mixed culture and PVA degradation mechanism. J. Food Sci. Biotechnol. 2006; 25, 73-8.

JRA Fried. A Text Book of Polymer Science and Technology. Prentice Hall PTR, New Jersey, USA, 1995.

NPGA Roeges. A Text Book of a Guide to the Complete Interpretation of Infrared Spectra of Organic Structures. Wiley & Sons, Chichester, England, 1994.

X Hu, R Mamoto, Y Shimomura, K Kimbara and F Kawai. Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3. Arch. Microbiol. 2007; 188, 235-41.

K Momma, M Okamota, Y Mishima, S Mori, W Hashimoto and K Murata. A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules. J. Bacteriol. 2000; 18, 3998-4004.

Published
2020-07-01
How to Cite
KANJANASOPA, D., PIMPA, B., THITITHANAKUL, S., & WISUNTHORN, S. (2020). Biodegradation of Polyvinyl Alcohol by Thai Indigenous Mixed Microbial Culture. Walailak Journal of Science and Technology (WJST), 17(7), 698-707. Retrieved from http://wjst.wu.ac.th/index.php/wjst/article/view/6158