Biodegradation of Polyvinyl Alcohol by Thai Indigenous Mixed Microbial Culture

Authors

  • Duangkhaetita KANJANASOPA Agricultural Science and Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
  • Benchamaporn PIMPA Food Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
  • Suraphon THITITHANAKUL Agricultural Science and Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand
  • Suwaluk WISUNTHORN Rubber Technology Program, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.6158

Keywords:

Biodegradation, Polyvinyl alcohol, PVA degrading microorganism, Mixed microbial cultures

Abstract

PVA is biodegradable plastic and a water-soluble synthetic polymer that plays a significant role in industry. A large amount of PVA in wastewater causes heavy environmental pollution in terms of accumulation, disposal, and long-term degradation; therefore it must be removed from wastewater before the water is discharged. In this study, NS3 mixed microbial culture, capable of completely degrading 5 g.L-1 polyvinyl alcohol (PVA), was isolated from landfill soil using the enrichment culture method. It completely degraded PVA at an initial concentration in the range 1 - 5 g.L-1 over 5 - 20 days of incubation with continuous shaking at 30 °C. Moreover, mixed microbial cultures were found to remove PVA at a high range concentration of 10 - 25 g.L-1. Urea and glucose added to the medium inhibited PVA degradation by increasing the pH to a strongly alkaline level, which would cause cell viability and enzyme stability. The FT-IR spectra and SEM imaging revealed the mechanisms and the physical degradation of PVA films, respectively. PVA uptake in bacterial cells produced a dent in the cell surface, which represented the consumption of PVA by bacterial cell. The PVA-degrading mixed microbial culture is the first reported in Thailand and can be beneficial in PVA wastewater treatment.

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Published

2020-07-01

How to Cite

KANJANASOPA, D. ., PIMPA, B. ., THITITHANAKUL, S. ., & WISUNTHORN, S. . (2020). Biodegradation of Polyvinyl Alcohol by Thai Indigenous Mixed Microbial Culture. Walailak Journal of Science and Technology (WJST), 17(7), 698–707. https://doi.org/10.48048/wjst.2020.6158