Evaluation of antioxidant activity and inhibition of tyrosinase activity of Raphanus sativus var. caudatus Alef extract

Natthida Weerapreeyakul


This study was aimed to determine antioxidant and tyrosinase inhibition effects of the pod of Raphanus sativus L. var. caudatus Alef extract.  The compounds consisted in the extracts were identified by HPLC from standard peak comparison.  Pod was extracted by using two difference solvents—dichloromethane (DCM) and water.  The antioxidant activity was evaluated based on free radical scavenging (DPPH) ability and ferric reducing ability (FRAP) assay.  The lightening effect was determined from the inhibition of mushroom tyrosinase in vitro.  Results showed that DCM extract contained sulforaphene, sulforaphane, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid and ferulic acid.  Aqueous extract contained sulforaphene, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid, vanillic acid and p-coumaric acid.  The DPPH· scavenging effect expressed as the IC50 values for DCM and aqueous extracts were 883.38±22.9 µg/ml and 1,160.49±22.30 µg/ml, respectively.  The reducing power of aqueous extract was greater than DCM extract at the same 2,000 µg/ml, which were expressed as 218.27±0.010 µM and 166.34±0.018 µM FeSO4 equivalent, respectively.  However, both extracts showed lesser antioxidant activity than gallic acid, a positive control.  DCM extract showed higher tyrosinase inhibitory effect than aqueous extract but lesser than kojic acid, a positive control.  Both extracts at 2,000 µg/ml concentration exerted respective 42% and 19% tyrosinase inhibition.  In conclusion, the extraction solvent influenced on the activities.  DCM extract exerted greater antioxidant activity and tyrosinase inhibition activity than aqueous extract.  The extract fractionation is required to get higher yields of bioactive compounds prior to further study and for cosmetics product development.


HPLC, antioxidant, antityrosinase, Raphanus sativus var. caudatus Alef, Thai rat-tailed radish


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Last updated: 29 December 2018