Evaluation of Antioxidant Activity and Inhibition of Tyrosinase Activity of Raphanus sativus var. caudatus Alef Extract

Authors

  • Panadda YONGPRADOEM Graduate School (in the Program of Aesthetic Sciences and Health), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
  • Natthida WEERAPREEYAKUL Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand http://orcid.org/0000-0002-0810-8425

Keywords:

HPLC, Antioxidant, Antityrosinase, Raphanus sativus var. caudatus Alef, Thai rat-tailed radish

Abstract

This study was aimed to determine antioxidant and tyrosinase inhibition effects of the pod of Raphanus sativus L. var. caudatus Alef extract. The compounds consisted in the extracts were identified by HPLC from standard peak comparison. Pod was extracted by using 2 different solvents-dichloromethane (DCM) and water. The antioxidant activity was evaluated based on free radical scavenging (DPPH) activity and ferric reducing ability (FRAP) assay. The lightening effect was determined from the inhibition of mushroom tyrosinase in vitro. The results showed that DCM extract contained sulforaphene, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid, and ferulic acid.  The aqueous extract contained sulforaphene, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid, vanillic acid, and p-coumaric acid.  The DPPH· scavenging effect expressed as the IC50 values for DCM and aqueous extracts were 883.38 ± 22.9 and 1,160.49 ± 22.30 µg/mL, respectively. The reducing power of aqueous extract (218.27 ± 0.010 µM FeSO4 equivalent) was greater than DCM extract (166.34 ± 0.018 µM FeSO4 equivalent) at the same concentration (2,000 µg/mL).  However, both extracts showed lesser antioxidant activity than gallic acid, a positive control. DCM extract showed higher tyrosinase inhibitory effect than aqueous extract but lesser than kojic acid, a positive control. Both extracts at 2,000 µg/mL concentration exerted 42 and 19 % tyrosinase inhibition, respectively. In conclusion, the extraction solvent yielded different chemical constituents and thus the activities. The DCM extract exerted greater antioxidant activity and tyrosinase inhibition activity than the aqueous extract. The extract fractionation is required to get higher yields of bioactive compounds prior to further study and for cosmetics product development.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biography

Natthida WEERAPREEYAKUL, Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand

Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, 123 Mittrapap Road, Amphoe Muang, Khon Kaen 40002, Thailand

References

B Desmedt, P Courselle, JO De Beer, V Rogiers, M Grosber, E Deconinck and KD Paepe. Overview of skin whitening agents with an insight into the illegal cosmetic market in Europe. J. Eur. Dermatol. Venereol. 2016; 30, 943-50.

GF Sun, WT Hu, ZH Yuan, BA Zhang and H Lu. Characteristics of mercury intoxication induced by skin-lightening products. Chin. Med. J.-Peking, 2017; 130, 3003-4.

C Couteau and L Coiffard. Overview of skin whitening agents: drugs and cosmetic products. Cosmetics 2016; 3, 27.

SN Chohan, M Suhail, S Salman, UM Bajwa, M Saeed, S Kausar and T Suhail. Facial abuse of topical steroids and fairness creams: A clinical study of 200 patients. J. Pak. Asso. Dermatol. 2014; 24, 204-11.

T Songsak and G Lockwood. Glucosinolates of seven medicinal plants from Thailand. Fitoterapia 2002; 73, 209-16.

P Pocasap, N Weerapreeyakul and S Barusrux. Cancer preventive effect of Thai rat-tailed radish (Raphanus sativus L. var. caudatus Alef). J. Funct. Food. 2013; 5, 1372-81.

S Sangthong, N Weerapreeyakul, M Lehtonen, J Leppanen and J Rautio. High-accuracy mass spectrometry for identification of sulphur-containing bioactive constituents and flavonoids in extracts of Raphanus sativus var. caudatus Alef (Thai rat-tailed radish). J. Funct. Food. 2017; 31, 237-47.

S Sangthong, N Weerapreeyakul and S Barusrux. Antiproliferative effect of Raphanus sativus L. var. caudatus Alef in human breast MCF-7 and human lung SK-LU1 adenocarcinoma cell lines. Isan J. Pharm. Sci. 2015; 10, 75-81.

S Sangthong, N Weerapreeyakul and S Barusrux. Cell survival inhibitory effect of Raphanus sativus v. caudatus Alef extracts against non-resistant HepG2 and resistant HepG2 hepatocellular carcinoma. In: Proceedings of the 34th National Graduate Research Conference, Khon Kaen University, Khon Kaen, Thailand. 2016, p. 80-3.

T Charoonratana, S Settharaksa, F Madaka and T Songsak. Screening of antioxidant activity and total phenolic content in Raphanus sativus pod. Int. J. Pharm. Pharmaceut. Sci. 2014; 6, 224-6.

A Siddiq and I Younus. Screening in-vitro antifungal activity of Raphanus sativus L. var. caudatus. World J. Pharm. Pharmaceut. Sci. 2015; 4, 429-37.

I Younus and A Siddiq. Behavioral evidence of antidepressant-like activity of Raphanus sativus L. var. caudatus in mice. Afr. J. Tradit. Complement. Altern. Med. 2017; 14, 142-6.

I Younus and A Siddiq. In-Vitro antileishmanial activity of Raphanus sativus L. var. caudatus. J. Basic Appl. Sci. 2016; 12, 242-4.

R Jakmatakul, R Suttisri and P Tengamnuay. Evaluation of antityrosinase and antioxidant activities of Raphanus sativus root: Comparison between freeze-dried juice and methanolic extract. Thai J. Pharmaceut. Sci. 2009; 33, 22-30.

M Jahangir, HK Kim, YH Choi and R Verpoorte. Health-affecting compounds in Brassicaceae. Compr. Rev. Food Sci. Food Saf. 2009; 8, 31-43.

S.Sangthong and N Weerapreeyakul. Simultaneous quantification of sulforaphene and sulforaphane by reverse phase HPLC and their content in Raphanus sativus L. var. caudatus Alef extracts. Food Chem. 2016; 201, 139-44.

O Kaisoon, S Siriamornpun, N Weerapreeyakul and N Meeso. Phenolic compounds and antioxidant activities of edible flowers from Thailand. J. Funct. Food. 2011; 3, 88-99.

W Brand-Williams, ME Cuvelier, and C Berset. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 1995; 28, 25-30.

IF Benzie and JJ Strain. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 1996; 239, 70-6.

M Srisayam, N Weerapreeyakul, S Barusrux, W Tanthanuch and K Thumanu. Application of FTIR microspectroscopy for characterization of biomolecular changes in human melanoma cells treated by sesamol and kojic acid. J. Dermatol. Sci. 2014; 73, 241-50.

F Shahidi and Y Zhong. Measurement of antioxidant activity. J. Funct. Food. 2015; 18, 757-81.

M Karamac, A Kosinska and RB Pegg. Comparison of radical-scavenging activities for selected phenolic acids. Pol. J. Food Nutr. Sci. 2005; 14, 165-70.

W Thangboonjit, T Pluemsamran and U Panich. Comparative evaluation of antityrosinase and antioxidant activities of dietary phenolics and their activities in melanoma cells exposed to UVA. Siriraj Med. J. 2014; 66, 5-10.

PD Duh, PC Du and GC Yen. Action of methanolic extract of mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food Chem. Toxicol. 1999; 37, 1055-61.

S Nishaa, M Vishnupriya, J Sasikumar, PC Hephzibah and V Gopalakrishnan. Antioxidant activity of ethanolic extract of Maranta arundinacea L. tuberous rhizomes. Asian J. Pharmaceut. Clin. Res. 2012; 5, 85-8.

K Csepregi, S Neugart, M Schreiner and E Hideg. Comparative evaluation of total antioxidant capacities of plant polyphenols. Molecules 2016; 21, 208.

YJ Kim and H Uyama. Tyrosinase inhibitors from natural and synthetic sources: Structure, inhibition mechanism and perspective for the future. Cell Mol. Life Sci. 2005; 62, 1707-23.

I Shirasugi, M Kamada, T Matsui, Y Sakakibara, MC Liu and M Suiko. Sulforaphane inhibited melanin synthesis by regulating tyrosinase gene expression in B16 mouse melanoma cells. Biosci. Biotechnol. Biochem. 2010; 74, 579-82.

M Miyazawa, T Oshima, K Koshio, Y Itsuzaki and J Anzai. Tyrosinase inhibitor from black rice bran. J. Agric. Food Chem. 2003; 51, 6953-6.

Downloads

Published

2020-08-01

How to Cite

YONGPRADOEM, P. ., & WEERAPREEYAKUL, N. . (2020). Evaluation of Antioxidant Activity and Inhibition of Tyrosinase Activity of Raphanus sativus var. caudatus Alef Extract. Walailak Journal of Science and Technology (WJST), 17(8), 838-850. Retrieved from http://wjst.wu.ac.th/index.php/wjst/article/view/5541