Effect of Semi-synthetic Andrographolide Analogue-loaded Polymeric Micelles on HN22 Cell Migration

Authors

  • Teeratas KANSOM Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Rungnapha SAEENG Department of Chemistry, Faculty of Science, Burapha University, Chonburi 20131, Thailand
  • Tanasait NGAWHIRUNPAT Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Theerasak ROJANARATA Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Prasopchai TONGLAIROUM Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Praneet OPANASOPIT Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Purin CHAROENSUKSAI Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand

Keywords:

Andrographolide analogue, polymeric micelles, cell migration, oral cancer

Abstract

Semi-synthetic andrographolide (AG) analogue, namely 19-tert-butyldiphenylsilyl-8,17-epoxy andrographolide, or 3A.1, is an anticancer drug. However, the major problem of 3A.1 is poor water solubility hindering its clinical applications. To improve the water solubility and anticancer potency of this analogue, 3A.1-loaded polymeric micelles employing N-naphthyl-N-O-succinyl chitosan (NSCS) as amphiphilic copolymer were prepared by the dropping method. The morphology, particle size, entrapment efficiency (%EE), and loading capacity (%LC) were evaluated. The 3A.1-loaded NSCS micelles were successfully prepared. These micelles were nano-size (66.26 to 102.53 nm) and with a spherical shape, with negative surface charge (-30.50 to -22.23 mV). The 3A.1-loaded NSCS micelles with 40 % drug loading exhibited the maximum values of both %EE (90.84 %) and %LC (25.95 %), indicating that a high amount of 3A.1 could be entrapped into the NSCS micelles. In addition, in vitro anticancer activity and cell migration assay on HN22 cells were evaluated. The 3A.1-loaded NSCS micelles exhibited stronger anticancer effect and cell migration suppression than the free drug. Therefore, these NSCS micelles containing 3A.1 may be potential nanocarriers for the treatment of oral cancer.

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Published

2018-12-03

How to Cite

KANSOM, T. ., SAEENG, R. ., NGAWHIRUNPAT, T. ., ROJANARATA, T. ., TONGLAIROUM, P. ., OPANASOPIT, P. ., & CHAROENSUKSAI, P. . (2018). Effect of Semi-synthetic Andrographolide Analogue-loaded Polymeric Micelles on HN22 Cell Migration. Walailak Journal of Science and Technology (WJST), 17(2), 88-95. Retrieved from http://wjst.wu.ac.th/index.php/wjst/article/view/5515