Investigation of Dielectric Barriers Discharge Plasma Jets for Bactericidal in Chronic Wounds

Authors

  • Pipath PORAMAPIJITWAT Nanoscience and Nanotechnology, Faculty of Science, Maejo University, Chiang Mai 50290 http://orcid.org/0000-0002-7043-8521
  • Phuthitorn THANA Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200
  • Dheerawan BOONYAWAN Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200
  • Keratiya JANPONG Applied Physics, Faculty of Science, Maejo University, Chiang Mai 50290
  • Sureeporn SARAPIROM Thailand Center of Excellence in Physics, Commission on Higher Education, Bangkok 10400

DOI:

https://doi.org/10.48048/wjst.2019.4785

Keywords:

Atmospheric pressure plasma, dielectric barrier discharge plasma jets, bactericidal, chronic wounds, S. Aureus

Abstract

The atmospheric pressure plasma technique has been recognized in health care for disinfection in wounds as well as that it can enhance wound healing and reduce pain in patient without side effects. In this study, Dielectric Barrier Discharge Plasma Jets (DBDJs) were used for bactericidal in vitro as well as the efficiency of bacteria killing were investigated using gram positive bacteria, Staphylococcus Aureus
(S. Aureus). The DBDJs plasma used He gas at flow rate at 1 L/min, pulse repetition rate between 50 to 110 Hz and exposure time 15 to 60 s for bactericidal. The studies of DBDJs utilized an Optical Emission Spectroscopy (OES) to identify radical species in the plasma. The results of the OES studies showed in DBDJs plasma N2, NO, He and OH radical groups were found. These radicals in plasma played an important role in bactericidal, including wound healing. The intensity of radical in plasma depends on the repetition rate applied by the plasma system. After DBDJs plasma exposure, plates were incubated at 37 °C. Repetition rate and time of plasma exposure were drastically reduced. With the increase in the repetition rate over 100 Hz or exposure time up to 60 s for bactericidal, the reduction of bacteria was increased up to 100 %. The large clear zone showed the efficiency of bacteria killed ability of the plasma.

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Author Biography

Pipath PORAMAPIJITWAT, Nanoscience and Nanotechnology, Faculty of Science, Maejo University, Chiang Mai 50290

Nanoscience and Nanotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand.

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Published

2018-12-22

How to Cite

PORAMAPIJITWAT, P., THANA, P., BOONYAWAN, D., JANPONG, K., & SARAPIROM, S. (2018). Investigation of Dielectric Barriers Discharge Plasma Jets for Bactericidal in Chronic Wounds. Walailak Journal of Science and Technology (WJST), 16(6), 409–414. https://doi.org/10.48048/wjst.2019.4785