Screening for Biological Activities of Spirogyra neglecta Water Extract

Atchariya YOSBOONRUANG; Acharaporn DUANGJAI; Doungporn AMORMLERDPISON; Jarupa VIYOACH

doi:10.48048/wjst.2020.4638

Authors

Atchariya YOSBOONRUANG Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
Acharaporn DUANGJAI Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
Doungporn AMORMLERDPISON Faculty of Fisheries Technology and Aquatic Resources, Maejo University, Chiang Mai 50290, Thailand
Jarupa VIYOACH Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.4638

Keywords:

Spirogyra, algae, anti-inflammation, antioxidant, pancreatic cholesterol esterase

Abstract

The freshwater green algae, S. neglecta, has been commonly used as a Northern Thai local food due to the fact that it is composed of several nutritional components. The aim of the present study was to investigate the phytochemical properties and biological activities of an S. neglecta water extract for application as a functional food or pharmaceutical ingredient. The phenolic and flavonoid contents of S. neglecta and its biological activities, including anti-oxidant, pancreatic cholesterol esterase, anti-inflammatory, cytotoxic, and antibacterial activities, were investigated in vitro. The results showed that the S. neglecta extract contained 157.92 mg GAE/g extract of phenolics and 10.10 mg CE/g extract of flavonoids. The radical scavenging activity of the S. neglecta extract exhibited an IC₅₀ of 7.46±1.17 µg/mL from DPPH assay and 224.20±1.06 µg/mL from ABTS assay. Meanwhile, trolox exhibited an IC₅₀ of 7.03±1.08 µg/mL from DPPH assay and 5.24±1.10 µg/mL from ATBS assay. Interestingly, the S. neglecta extract at 10 mg/mL inhibited pancreatic cholesterol esterase activity by 60 %. Moreover, the release of TNF-α from macrophages was strongly reduced by incubation with the S. neglecta extract in a dose-dependent manner, and all of the study concentrations were non-toxic to primary fibroblast cells. In addition, the S. neglecta extract inhibited some gram-positive and -negative bacteria. In summary, S. neglecta extract could possibly be used as a potential functional food or pharmaceutical ingredient, for which further studies are required.

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