Colonization by Cupriavidus taiwanensis KKU2500-3 Enhances the Growth and Yield of KDML105 Jasmine Rice

Putthita Punjee PUNJEE; Wilailak  SIRIPORNADULSIL; Surasak SIRIPORNADULSIL

doi:10.48048/wjst.2020.4401

Authors

Putthita Punjee PUNJEE Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
Wilailak SIRIPORNADULSIL Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand
Surasak SIRIPORNADULSIL Salt-Tolerant Rice Research Group, Khon Kaen University, Khon Kaen 40002, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.4401

Keywords:

Cupriavidus taiwanensis KKU2500-3, KDML105 jasmine rice, colonization, hydroponic system

Abstract

The effects of the cadmium-tolerant bacterium Cupriavidus taiwanensis KKU2500-3 on the growth and grain production of jasmine rice (Oryza sativa L. var. KDML105) were studied. Bacterial cells were inoculated onto rice seedlings before transplanting into a hydroponic system; the cells successfully colonized, became distributed, and multiplied in the range of 10⁴ - 10⁹ log CFU·g^-1 plant. The bacterial cells were localized to the cell wall and the intercellular space of all plant parts. Moreover, inoculation of strain KKU2500-3 significantly promoted rice growth by increasing the length, dry mass of shoots and roots, and chlorophyll concentration in leaves, and improved yields by increasing the panicle length, number of seeds/panicle, number of filled grains/panicle, filled grain percentage, and 100-grain weight (13.57, 11.90, 48.20, 32.55, and 23.53 % over the control, respectively). The number of filled grains/panicle and grain weight accounted for the increase in total yield. C. taiwanensis KKU2500-3 fixed nitrogen, produced indole-3-acetic acid, and solubilized phosphate, affecting the growth and yield of all plants. Successful colonization of rice seedlings before transplanting is an important finding, as it will simplify bacterial inoculation of plants, especially when grown in cadmium-contaminated rice fields.

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