Colonization by Cupriavidus taiwanensis KKU2500-3 Enhances the Growth and Yield of KDML105 Jasmine Rice

  • Putthita Punjee PUNJEE Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
  • Wilailak SIRIPORNADULSIL Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand
  • Surasak SIRIPORNADULSIL Salt-Tolerant Rice Research Group, Khon Kaen University, Khon Kaen 40002, Thailand
Keywords: Cupriavidus taiwanensis KKU2500-3, KDML105 jasmine rice, colonization, hydroponic system


The effects of the cadmium-tolerant bacterium Cupriavidus taiwanensis KKU2500-3 on the growth and grain production of jasmine rice (Oryza sativa L. var. KDML105) were studied. Bacterial cells were inoculated onto rice seedlings before transplanting into a hydroponic system; the cells successfully colonized, became distributed, and multiplied in the range of 104 - 109 log CFU·g-1 plant. The bacterial cells were localized to the cell wall and the intercellular space of all plant parts. Moreover, inoculation of strain KKU2500-3 significantly promoted rice growth by increasing the length, dry mass of shoots and roots, and chlorophyll concentration in leaves, and improved yields by increasing the panicle length, number of seeds/panicle, number of filled grains/panicle, filled grain percentage, and 100-grain weight (13.57, 11.90, 48.20, 32.55, and 23.53 % over the control, respectively). The number of filled grains/panicle and grain weight accounted for the increase in total yield. C. taiwanensis KKU2500-3 fixed nitrogen, produced indole-3-acetic acid, and solubilized phosphate, affecting the growth and yield of all plants. Successful colonization of rice seedlings before transplanting is an important finding, as it will simplify bacterial inoculation of plants, especially when grown in cadmium-contaminated rice fields.


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F Ahmad, I Ahmad, MS Khan. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 2008; 163, 173-81.

C Contesto, S Milesi, S Mantelin, A Zancarini, G Desbrosses, F Varoquaux, C Bellini, M Kowalczyk and B Touraine. The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum. Planta 2010; 232, 1455-70.

WM Chen, S Laevens, TM Lee, T Coenye, PD Vos, M Mergeay and P Vandamme. Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int. J. Syst. Evol. Microbiol. 2001; 51, 1729-35.

WM Chen, L Moulin, C Bontemps, P Vandamme, G Béna and C Boivin-Masson. Legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature. J. Bacteriol. 2003; 185, 7266-72.

S Siripornadulsil and W Siripornadulsil. Cadmium-tolerant bacteria reduce the uptake of cadmium in rice: potential for microbial bioremediation. Ecotoxicol. Environ. Saf. 2013; 94, 94-103.

P Punjee, W Siripornadulsil and S Siripornadulsil. Effects of cadmium-resistant bacteria, Cupriavidus taiwanensis KKU2500-3, on Thai jasmine rice to cadmium toxicity. Curr. Opin. Biotech. 2013; 24, S28-S47.

P Punjee. 2016, Effects of Cadmium-resistant Bacteria on Rice Plant Growth and Its Cadmium Accumulation. Ph. D. Thesis, Khon Kaen University, Khon Kaen, Thailand.

JC Cappuccino and N Sherman. Negative Staining. In: JC Cappuccino and N Sherman (Eds.). Microbiology: A Laboratory Manual. 3rd eds. Benjamin/Cummings, Redwood City, 1992, p. 125-79.

JM Bric, RM Bostock and SE Silverstone. Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl. Environ. Microbiol. 1991; 57, 535-8.

B Schwyn and JB Neilands. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 1987; 160, 47-56.

RI Pikovskaya. Mobilization of phosphorus in soils in connection with vital activity of some microbial species. Mikrobiologiya 1948; 17, 362-70.

AH Goldstein. Bacterial solubilization of mineral phosphates: Historical perspective and future prospects. Am. J. Alter. Agr. 1986; 1, 51-7.

EJ King. The colorimetric determination of phosphorus. Biochem. J. 1932; 26, 292-7.

EK James, P Gyaneshwar, N Mathan, WL Barraquio, PM Reddy, PP Iannetta, FL Olivares and JK Ladha. Infection and colonization of rice seedlings by the plant growth-promoting bacterium Herbaspirillum seropedicae Z67. Mol. Plant Microbe Interact. 2002; 15, 894-906.

DR Hoagland and DI Arnon. The water-culture method for growing plants without soil, Circular. Calif. Agr. Exp. Stat. 1950; 347, 1-32.

KA Mattos, VL Pádua, A Romeiro, LF Hallack, BC Neves, TM Ulisses, CF Barros, AR Todeschini, JO Previato and L Mendonça-Previato. Endophytic colonization of rice (Oryza sativa L.) by the diazotrophic bacterium Burkholderia kururiensis and its ability to enhance plant growth. An. Acad. Bras. Cienc. 2008; 80, 477-93.

HK Lichtenthaler and C Buschmann. Chlorophylls and Carotenoids: Measurement and Characterization by UV-vis Spectroscopy. In: HK Lichtenthaler and C Buschmann. (Eds.). Current Protocols in Food Analytical Chemistry. John Wiley & Sons, New York, 2001, p. 24-56.

S Yao, S Taketa and M Ichii. A novel short-root gene that affects specifically early root development in rice (Oryza sativa L.). Plant Sci. 2002; 163, 207-15.

C Yang, L Yang, Y Yang and Z Ouyang. Rice root growth and nutrient uptake as influenced by organic manure in continuously and alternately flooded paddy soils. Agr. Water Manag. 2004; 70, 67-81.

B Lippmann, V Leinhos and H Bergmann. Influence of auxin producing rhizobacteria on root morphology and nutrient accumulation of crops. I: Changes in root morphology and nutrient accumulation in maize caused by inoculation with indole-3-acetic acid producing Pseudomonas and Acinetobacter strains or IAA applied exogenously. Angew. Bot. 1995; 69, 31-6.

DP Sachdev, HG Chaudhari, VM Kasture, DD Dhavale and BA Chopade. Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. Indian J. Exp. Biol. 2009; 47, 993-1000.

J Yang, H Zhang and J Zhang. Root morphology and physiology in relation to the yield formation of rice. J. Integr. Agr. 2012; 11, 920-6.

S Spaepen, J Vanderleyden and R Remans. Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol. Rev. 2007; 31, 425-48.

M Bacilio-Jiménez, S Aguilar-Flores, E Ventura-Zapata, E Pérez-Campos, S Bouquelet and E Zenteno. Chemical characterization of root exudates from rice (Oryza sativa) and their effects on the chemotactic response of endophytic bacteria. Plant Soil 2003; 249, 271-7.

M Marchetti, O Catrice, J Batut and C Masson-Boivin. Cupriavidus taiwanensis bacteroids in mimosa pudica indeterminate nodules are not terminally differentiated. Appl. Environ. Microbiol. 2011; 77, 2161-4.

A Klonowska, C Chaintreuil, P Tisseyre, L Miché, R Melkonian, M Ducousso, G Laguerre, B Brunel and L Moulin. Biodiversity of mimosa pudica rhizobial symbionts (Cupriavidus taiwanensis, rhizobium mesoamericanum) in New Caledonia and their adaptation to heavy metal-rich soils. FEMS Microbiol. Ecol. 2012; 81, 618-35.

M Stella and M Suhaimi. Selection of suitable growth medium for free-living diazotrophs isolated from compost. J. Trop. Agr. Food Sci. 2010; 38, 211-9.

B Lugtenberg, N Malfanova, F Kamilova and G Berg. Plant Growth Promotion by Microbes. In: FJ de Bruijn (Ed.). Molecular Microbial Ecology of the Rhizosphere, Wiley Blackwell, Hoboken, NJ, 2013, p. 561-73.

J Fan, Y Zhang, D Turner, Y Duan, D Wang and Q Shen. Root physiological and morphological characteristics of two rice cultivars with different nitrogen-use efficiency. Pedosphere 2010; 20, 446-55.

BB Bohlool, JK Ladha, DP Garrity and T George. Biological nitrogen fixation for sustainable agriculture: A perspective. Plant Soil 1992; 141, 1-11.

KR Reddy and WHJ Patrick. Yield and nitrogen utilization by rice as affected by method and time of application of labelled nitrogen. Agron. J. 1976; 68, 965-9.

How to Cite
PUNJEE, P. P., SIRIPORNADULSIL, W., & SIRIPORNADULSIL, S. (2018). Colonization by Cupriavidus taiwanensis KKU2500-3 Enhances the Growth and Yield of KDML105 Jasmine Rice. Walailak Journal of Science and Technology (WJST), 17(1), 23-36. Retrieved from