Enhancement of the Enzymatic and Non-Enzymatic Antioxidant Activities of Cultured Sauropus androgynus L. Merr. Shoots Treated with UV-C Irradiation

Ratchanee Petchang


The stimulation of enzymatic and non-enzymatic antioxidant activities of Sauropus androgynus L. Merr. shoot cultures treated with UV-C irradiation was investigated. The shoots were first cultured on Murashige and Skoog (MS) medium containing 1 mg/L 6-benzyladenine (BA) for six weeks. Multiple shoot formations were then treated with UV-C irradiation for 0 min (control group), 5, 10 min (experimental groups) and wild type. After three weeks of culture the shoots were extracted with methanol and analyzed for enzymatic antioxidant activities (with superoxide dismutase; SOD and peroxidase; POD) and non-enzymatic antioxidant activities (with DPPH, ABTS and FRP assays), total phenolic and flavonoid compound contents. The result indicate that the shoot treated with UV-C irradiation for 5 min exhibited the highest enzymatic antioxidant activities (SOD and POD values of 2.26, 2.37 unit/mg proteins, respectively). Non-enzymatic antioxidant activities determined by using DPPH, ABTS and FRP assays was found to very form IC50 values were 10.13 mg/mL, 166.48 mg TEAC/100 g fresh weight (FW) and 22.72 mM FeSO4 /100 g FW, respectively. Total phenolic and flavonoid compound contents had values at 15.54 mg GAE/100 g FW and 63.64 mg QE/100 g FW, respectively. Antioxidant activities revealed a positive moderate high correlation (r2 = 0.729 - 0.980).


Sauropus androgynus L. Merr., enzymatic and non-enzymatic antioxidant activities, UV-C irradiation, tissue culture


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Last updated: 13 February 2019