Reproductive Aspects of SPF Penaeus monodon Grown in Closed Culture Captivity

Piyapong CHOTIPUNTU; Suwit WUTHISUTHIMETHAVEE; Sataporn DIREKBUSRAKOM; Satit SONGTUAY

Authors

Piyapong CHOTIPUNTU School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161
Suwit WUTHISUTHIMETHAVEE School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161
Sataporn DIREKBUSRAKOM School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161
Satit SONGTUAY Shrimp Research Unit, Walailak University, Nakhon Si Thammarat 80161

Keywords:

Broodstock, maturation, multiple spawning, Penaeus monodon, specific pathogen free

Abstract

Reproductive performance of P. monodon in terms of viable spermatophore, ovarian development and spawning characteristics was examined. The specific pathogen free (SPF) larvae were obtained from the SPF wild caught broodstock spawned in captivity. The SPF post-larvae were reared to mature entirely in the disinfected medium in circulation system. It was found that male shrimp matured far earlier than female shrimp. Maturation of male shrimp was determined by appearing of the ampoules observed between the fifth pereiopod and the first pleopod. The first maturation of male was observed within 6 months at an average body weight of 11 ± 2 g (n = 6). Sperm quantity and quality were examined in shrimp of 12 months old with an average body weight of 48 ± 3 g (n = 5). The average sperm count per spermatophore was 29.7 ± 16.5×10⁶ with viability of 79.0 ± 11.1 %. Maturation of female shrimp was determined by appearing of the gravid ovaries of stage 3 - 4 observed on dorsal part of abdominal segments. The first maturation of female was found within 16 months with an average body weight of 78 ± 4 g (n = 2). The other group of 12 month-old female shrimp was manipulated to mature using eyestalk ablation and artificial insemination techniques. Spawning characteristics of the ablated shrimp found to vary among individuals. A number of ablated female exhibited multiple spawning up to 3 consecutive times with a single insemination. Numbers of egg and percentages of hatch apparently decreased in the later spawning in the group of shrimp spawned 3 consecutive times with single insemination (p > 0.05). Egg counts in each spawning varied from 79,000 to 260,000 showed no particular pattern of relationship with body weight. Hatching feasibility varied from nil to 85 %. This study demonstrated the promising potential in producing specific pathogen free broodstock in tropical climate environment. However, further research is needed to enhance growth performance and survival rate. The quality of spawn in terms of viable spermatophore and ovarian development is yet to be improved.

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Author Biographies

Piyapong CHOTIPUNTU, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161

Assitant Professor, Chair of Graduate School Program in Agricultural Science

Suwit WUTHISUTHIMETHAVEE, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161

School of Agricultural Technology

Sataporn DIREKBUSRAKOM, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161

School of Agricultural Technology

Satit SONGTUAY, Shrimp Research Unit, Walailak University, Nakhon Si Thammarat 80161

Shrimp Research Unit

References

Department of Fisheries. Fisheries Statistic of Thailand in 2007. Department of Research and Analysis, Information Center (in Thai) 2007.

Aquacop. Penaeid reared broodstock: Closing the cycle of Penaeus momodon, P. stylirostris and P. vannamei. Proceedings of the World Mariculture Society 1979; 10, 445-52.

GJ Coman, PJ Crocos, SJ Arnold, SJ Key and NP Preston. Growth, survival and reproductive performance of domesticated Australian stock of the giant tiger prawn, Penaeus monodon, reared in tanks and raceways. J. World Aquaculture Soc. 2005; 36, 464-79.

D Yan, KFJ Tang and DV Lightner. Development of a real-time PCR assay for detection of monodon baculovirus (MBV) in penaeid shrimp. J. Invertebr. Pathol. 2009; 102, 97-100.

ND Hoa, R Wouters, M Wille, V Thanh, TK Dong, NV Hao and P Sorgeloos. A fresh-food maturation diet with an adequate HUFA composition for broodstock nutrition studies in black tiger shrimp Penaeus monodon (Fabricius, 1798). Aquaculture 2009; 297, 116-21.

AG Hillier. Artificial conditions influencing the maturation and spawning of sub-adult Penaeus monodon (Fabricius). Aquaculture 1984; 36, 179-84.

JR Leung-Trujillo and AL Lawrence. Observation on the decline in sperm quality of Penaeus setiferus under laboratory conditions. Aquaculture 1987; 65, 363-70.

P Talbot, D Howard, J Leung-Trujillo, TW Lee, WY Li, H Ro and AL Lawrence. Characterization of male reproductive tract degenerative syndrome in captive penaeid shrimp (Penaeus setiferus). Aquaculture 1989; 78, 365-77.

BP Ceballos-Vázquez, C Rosas and IS Racotta. Sperm quality in relation to age and weight of white shrimp Litopenaeus vannamei. Aquaculture 2003; 228, 141-51.

IG Anderson. Prawn Anatomy, Histology and Life History Biology. In: Invertebrate in Aquaculture, University of Sydney, Australia, 1989, p. 89-100.

SG Jiang, JH Huang, FL Zhou, X Chen, QB Yang, WG Wen and ZM Ma. Observations of reproductive development and maturation of male Penaeus monodon reared in tidal and earthen ponds. Aquaculture 2009; 292, 121-8.

JT Ogle. A review of current (1992) state of our knowledge concerning reproduction in open thelycum penaeid shrimp with emphasis on Litopenaeus vannamei. Invertebr. Reprod. Dev. 1992; 22, 267-74.

Y Goimier, C Pascual, A Sánchez, G Gaxiola, A Sánchez and C Rosas. Relation between reproductive, physiological, and immunological condition of Litopenaeus setiferus pre-adult males fed different dietary protein levels (Crustacea; Penaeidae). Anim. Reprod. Sci. 2006; 92. 193-208.

R Wouters, P Lavens, J Nieto and P Sorgeloos. Penaeid shrimp broodstock nutrition: An updated review on research and development. Aquaculture 2001; 202, 1-21.

JH Primavera. Induced maturation and spawning in five-month-old Penaeus monodon Fabricius by eyestalk ablation. Aquaculture 1978; 13, 355-9.

BP Ceballos-Vázquez, E Palacios, J Aguilar-Villavicencio and IS Racotta. Gonadal development in male and female domesticated whiteleg shrimp, Litopenaeus vannamei, in relation to age and weight. Aquaculture 2010; 308, 116-23.

G Marsden, P Mather and N Richardson. Captivity, ablation and starvation of the prawn Penaeus monodon affects protein and lipid content in ovary and hepatopancreas tissues. Aquaculture 2007; 271, 507-15.

GJ Coman, SJ Arnold, TR Callaghan and NP Preston. Effect of two maturation diet combinations on reproductive performance of domesticated Penaeus monodon. Aquaculture 2007; 263, 75-83.

RT Bauer and LJ Min. Spermatophores and plug substance of the marine shrimp Trachypenaeus similes (Crustacean: Decapoda: Penaeidae): Formation in male reproductive tract and disposition in the inseminated female. Biol. Bull. 1993; 185, 174-85.

SL Anderson, WH Clark Jr and ES Chang. Multiple spawning and molt synchrony in a free spawning shrimp (Sicyonia ingentis: Penaeoidea). Biol. Bull. 1985; 168, 377-94.

E Palacios, AM Ibarra and IS Racotta. Tissue biochemical composition in relation to multiple spawning in wild and pond-reared Penaeus vannamei broodstock. Aquaculture 2000; 185, 353-71.