Production and Properties of Biosurfactant from Pectinatus cerevisiiphilus CT3 Isolated from Marine Sediments

Authors

  • Chanika SAENGE CHOOKLIN Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang Campus, Trang 90000, Thailand
  • Atipan SAIMMAI Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket 83000, Thailand

DOI:

https://doi.org/10.48048/wjst.2020.4229

Keywords:

Production, Antimicrobial activity, Biosurfactant, Pectinatus cerevisiiphilus, Marine bacteria

Abstract

A marine bacterial isolate, Pectinatus cerevisiiphilus CT3, was able to grow and produce biosurfactant on minimal salts media using glucose and NaNO3 as carbon and nitrogen sources. It was found that cellular growth and biosurfactant production in MSM were greatly affected by the medium components. After 54 h of cultivation, P. cerevisiiphilus CT3 was able to grow and produce surfactant, reducing the surface tension of the medium to 28.0 mN/m with a biosurfactant concentration of 3.05 g/l and a critical micelle concentration of 10 mg/l. Biosurfactant recovery by chloroform/methanol extraction showed pH and thermal stability with respect to surface tension reduction. It also showed emulsification activity and a high level of salt concentration. In addition, promising antimicrobial activity was revealed when tested against human pathogenic bacterial and fungal isolates. Based on these results, the isolated biosurfactant from the marine bacteria P. cerevisiiphilus CT3 revealed a broad physicochemical stability and has excellent antimicrobial properties, indicating the potential for possible use in various therapeutic and biomedical applications.

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Published

2020-06-01

How to Cite

SAENGE CHOOKLIN, C. ., & SAIMMAI, A. . (2020). Production and Properties of Biosurfactant from Pectinatus cerevisiiphilus CT3 Isolated from Marine Sediments. Walailak Journal of Science and Technology (WJST), 17(6), 543–558. https://doi.org/10.48048/wjst.2020.4229