Prevalence and characterization of pathogenic bacteria in bulk tank raw milk, Thailand

Chanida Kupradit, Sasidhorn Innok, Jirayus Woraratphoka, Mariena Ketudat-Cairns

Abstract


There are numerous numbers of raw milk collection centers in Nakhon Ratchasima but lack information of the isolation and characterization of foodborne pathogens in raw milk. Therefore, the purpose of this research was to investigate the prevalence and characterization of foodborne pathogens including Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus from 33 raw milk samples from 9 different raw milk collection centers located in 8 districts in Nakhon Ratchasima, Thailand, between January to March 2016. Contaminations of L. monocytogenes and Salmonella spp. were not detected in any of the raw milk sample tested. The prevalence of B. cereus, E. coli, and S. aureus in raw milk samples were found to be 9 % (10 – 2.0 × 104 CFU/ml), 42.4 %, and 54 % (85 – 2.7 × 104 CFU/ml), respectively. The distribution of virulence genes were tested in B. cereus and S. aureus using gene specific primers by polymerase chain reaction. Out of the 29 analyzed coagulase-positive S. aureus isolates, 27 isolates (93 %) were positive for eap gene amplification and 14 isolates (48 %) showed amplicon of eap gene and all 5 enterotoxin genes including seG, seGV, seI, seIV, and seM genes. All 8 B. cereus isolates tested showed positive PCR result with enteroxin FM (entFM) gene but negative with hemolysin gene (hblA and hblD genes) amplifications. It was inferred from these studies that bulk tank milk is a potential source of S. aureus and B. cereus in milk.

Keywords


foodborne pathogens, raw milk, raw milk collection center, virulence genes

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