Evaluation of Antifungal Activity of Antagonistic Bacteria against Butt Rot Disease Pathogen of Pineapple

Mat Nawi NURNADIRAH; Kartini Che Mohd Ramli NENI; Mohd Yunus Nor YUZIAH

doi:10.48048/wjst.2018.4040

Authors

Mat Nawi NURNADIRAH Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Selangor
Kartini Che Mohd Ramli NENI Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA Pahang, Jengka, Pahang
Mohd Yunus Nor YUZIAH Department of Wood Science and Technology, Faculty of Applied Science, Universiti Teknologi MARA Pahang, Jengka, Pahang

DOI:

https://doi.org/10.48048/wjst.2018.4040

Keywords:

Thielaviopsis paradoxa, antifungal activity, antagonist bacteria, biocontrol agent, butt rot

Abstract

Butt rot disease, caused by Thielaviopsis paradoxa (De Seynes) Hohn., is one of the major diseases in pineapple cultivation in Malaysia. The objectives of this study were to evaluate the antifungal effect of antagonist bacteria against T. paradoxa, a causal agent of butt rot disease, and to observe the mechanism of antifungal activity of tested antagonist bacteria microscopically. In this study, in vitro antifungal potential of 5 antagonist bacteria, namely B1, B2, B3, B4, and B5, were isolated from infected and non-infected soil samples and evaluated using dual culture method against T. paradoxa. The mechanisms of antifungal activities of antagonist bacteria against the pathogen were microscopically observed. All of the bacteria showed inhibitory effects against the pathogenic fungi. B1 bacteria showed the highest inhibitory potential, with 73 % inhibition, followed by B2, B3, B4, and B5, with 71, 57, 56, and 48 % of inhibition compared to control, respectively. The results also showed that B2, B3, and B4 bacteria exhibited positive inhibition towards the pathogen, with more than 50 % percentage inhibition. The development of a new product for use as a biocontrol agent, used as an additional control or used in combination with existing ones, may reduce dependency on chemical control and increase antagonistic activity efficiency.

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Author Biography

Mat Nawi NURNADIRAH, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Selangor

Faculty of Plantation and Agriculture

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