Callus Induction and Plantlet Regeneration Systems in Indica Rice (Oryza sativa L.) Cultivar Sangyod

Authors

  • Thi Linh HO Faculty of Agriculture and Applied Biology, Can Tho University, Can Tho
  • Sompong TE-CHATO Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112
  • Sureerat YENCHON Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112

DOI:

https://doi.org/10.48048/wjst.2018.3772

Keywords:

Indica rice, Sangyod, 2, 4-D, L-proline, casein hydrolysate

Abstract

This study was conducted to determine the optimum concentrations of 2, 4-D, L-proline and casein hydrolysate (CH) for efficient callus induction and plantlet regeneration from culturing mature embryos of Sangyod, an economically important indica rice variety in Thailand. The highest frequency of callus induction (73.08 ± 2.65 %) was obtained from MS medium supplemented with 2 mg/L 2,4-D, 750 mg/L CH and 200 mg/L L-proline. The combination of 1 mg/L BA, 0.5 mg/L Kn and 0.5 mg/L NAA containing solidified MS medium gave the maximum mean fresh weight of callus (938.9 ± 44 mg), green spot formation (64.17 ± 7.08 %), shoot induction frequency (66.25 ± 6.80 %) and mean number of shoots/explant (6.12 ± 0.36 shoots). The greatest mean number of shoots/explant (14.93 ± 0.97 shoots) and root formation percentage (82.71 ± 3.03 %) was observed in liquified MS medium supplemented with 0.5 mg/L NAA and 1 mg/L BA.

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Author Biographies

Thi Linh HO, Faculty of Agriculture and Applied Biology, Can Tho University, Can Tho

Now, I am working in Department of Plant Science, Faculty Natural Resources, Prince of Songkla University, Hat Yai Campus, Songkla, Thailand

Sompong TE-CHATO, Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112

 Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai City, Songkhla Province, 90112 Thailand

Sureerat YENCHON, Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112

Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai City, Songkhla Province, 90112 Thailand

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Published

2017-11-03

How to Cite

HO, T. L., TE-CHATO, S., & YENCHON, S. (2017). Callus Induction and Plantlet Regeneration Systems in Indica Rice (Oryza sativa L.) Cultivar Sangyod. Walailak Journal of Science and Technology (WJST), 15(10), 753–763. https://doi.org/10.48048/wjst.2018.3772

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Research Article