Physicochemical Characteristics of Glucosamine from Blue Swimming Crab (Portunus pelagicus) Shell Prepared by Acid Hydrolysis

Authors

  • Jittrakan KRAISANGSRI Faculty of Agro-Industry, King Mongkut’s Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520
  • Sitthipong NALINANON Faculty of Agro-Industry, King Mongkut’s Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520 http://orcid.org/0000-0003-1047-0260
  • Siriporn RIEBROY Food and Nutrition Program, Faculty of Agriculture, Kasetsart University, Bangkok 10900
  • Suthasinee YARNPAKDEE Division of Marine Product Technology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100
  • Palanivel GANESAN Nanotechnology Research Center, Department of Biotechnology and Applied Life Science, College of Bio-medical and Health Science, Konkuk University Glocal campus, Chungju 380701

DOI:

https://doi.org/10.48048/wjst.2018.3666

Keywords:

Blue swimming crab, characterization, crab shell, FTIR, glucosamine, HPLC

Abstract

The aim of this research was to characterize glucosamine hydrochloride (GluHCl) from the shell of blue swimming crab (Portunus pelagicus). The crab shell was finely milled and processed to chitin prior to HCl hydrolysis using 30 % HCl for 30 min at 100 ºC for glucosamine production. The resultant glucosamine was recovered by crystallization using 95 % ethanol and was dried in a hot air oven. The color of the glucosamine crystals, expressed as L*, a*, and b*, was 83.01, 5.03, and -3.38, respectively. Crab shell glucosamine had high purity, which could be strongly stained by ninhydrin and presented at the same Rf of standard D-glucosamine using thin layer chromatography. Furthermore, prepared glucosamine exhibited similar Fourier transform infrared (FTIR) spectrum as standard D-glucosamine. Glucosamine from blue swimming crab shell had high purity as determined by HPLC and contained 808.15 mg D-glucosamine/g sample. The maximal transition temperature (Tmax) and the total enthalpy (ΔH) of prepared glucosamine were 194 ºC and 754.42 J/g, respectively. As a consequence, with the presented method, the resultant glucosamine was characterized to be D-glucosamine. Therefore, blue swimming crab shell, a byproduct from crab meat processing, has high potential as a raw material to produce glucosamine for food and nutraceutical applications.

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Published

2017-11-29

How to Cite

KRAISANGSRI, J., NALINANON, S., RIEBROY, S., YARNPAKDEE, S., & GANESAN, P. (2017). Physicochemical Characteristics of Glucosamine from Blue Swimming Crab (Portunus pelagicus) Shell Prepared by Acid Hydrolysis. Walailak Journal of Science and Technology (WJST), 15(12), 869–877. https://doi.org/10.48048/wjst.2018.3666

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Research Article