Utilization of Paper Cone Water Cups as a Lignocellulosic Waste for Alternative Substrates on Pleurotus ostreatus Production

suvit suwanno, Aminoh Ayae, Nuttida Suwanno


The utilization of paper cone water cups was examined as an alternative substrate for oyster mushroom (Pleurotus ostreatus) production. Bioconversion technology is an attractive and profitable method for converting lignocellulosic residue from municipal solid waste into protein rich biomass, thereby reducing waste paper and also enhancing environmental quality.The most suitable substrate for mycelial growth was waste paper from cone water cups (WPC), combined with rubber wood sawdust (RWS) in the ratio 75%:25% dry basis. The substrate mixture was adjusted for moisture content at 70%, the C/N ratio was fixed at 20:1 by adding urea, and supplemented with 8% rice bran. Spawn running used 10% seed inoculum.Cultivation was conducted in polyvinyl chloride (PVC)boxes, incubated at 25°C in dark condition with relative humidity maintained at 70-80%. The fastest spawn running (mycelia development) was at 5 days, with pin head formation at 9 days and fruiting body formation at 12 days, with the highest yield recorded at 26.59 g.Under these conditions, the potential of lignocellulosic waste conversion (biological efficiency) was recorded as 88.64%, and the protein content of P. ostreatus was 35.75% at  12 days cultivation.


lignocellulosic waste, waste utilization, bioconversion, sustainable food security, Pleurotus ostreatus,


. A. Sharp and J. Sang-Arun. A guide for sustainable urban organic waste management in Thailand: Combining food, energy, and climate co-benefits.IGES Policy report. Asia-Pacific Network for Global Change Research (APN) Thailand. 2012, p 17-43.

. A. Challcharoenwattana and C. Pharino. Co-Benefits of Household Waste Recycling for Local

Community’s Sustainable Waste Management in Thailand. Sustainability. 2015; 7: 7417-7437

. T. Chinda, N. Leewattana and N. Leeamnuayjaroen. The sudy of Landill sitable in Thailand. 1st Mae Fah Lunng University International Conference, Chiang Rai, Thailand. 2012, p.1-8.

. C. Chiemchairi, J. P. Juanga and C. Visvanathan. Municipal solid waste management in Thailand and disposal emission inventory. Environ Monit Assess. 2007; 135:13-20

. M. Badu, S. K. Twumasi and N. O. Boadi. Effect of lignocellulosic in wood used as substrate on the quality yield of mushroom. Food Nutr Sci. 2011, 2(7):780-784.

. V. P. Mane, S. S. Patil, A. A. Syed and M.M.V. Baig. Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju (Fr.) Singer. J Zhejiang Univ Sci B. 2007; 8: 745-751.

. S. Yildiz, Ü. C. Yildiz, E. D. Gezer and A. Temiz. Some lignocellulosic wastes uses as raw material in cultivation of the Pleurotus ostreatus culture mushroom. Process Biochem. 2002; 38: 301-306.

. R. Naraian, M. P Singh and S. Ram. Supplementation of basal substrate to boost up substrate strength and Oyster mushroom yield: An overview of substrates and supplements. Int J Curr Microbiol Appl Sci. 2016; 5:543-553.

. E. A. Adebayo and D. Martinez-Carrera. Oyster mushrooms (Pleuurotus) are useful for utilizing lignocellulosic biomass. Afr J Biotechnol, 2015; 14:52-67. (9)

. M. Dashban, H. Schrft and W. Qin. Fungal bioconversion of lignocellulosic residues; opportunities and pre-spectives. Int J Biol Sci. 2009; 5: 578-595.

. R. Zhang, X. Li and J.G. Fadel. Oyster mushroom cultivation with rice and wheat straw. Bioresour Technol. 2002; 82: 277-284.

. A. N. Philippoussis, G. Zervakis and P. Diamantopoulou. Bioconversion of agricultural lignocllulosic waste through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World J Microbiol Biotechnol. 2001; 17: 191-200.

. R. P. Tengerdy and G. Szakacs. Bioconversion of lignocellulose in solid substrate fermentation. Biochem. Eng. J. 2003;13: 169-179.

. Q. A. Mandeel, A. A. Al-Laith and S. A. Mohamed. Cultivation on oyster mushroom (Pleurotus spp.) on various lignocellulosic wastes. World J Microbiol Biotechnol. 2005; 21:601-607.

. E. Harada, T. Morizono and T. Sumiya. Production of andean-patagonic edible mushroom Grifola gargal on wood-based substrates. Mycosience. 2015; 56:616-621.

. E. Baysal H. Peker, M. M. Yalinkiliҫ and A. Temiz. Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresour Technol. 2003; 89: 95-97.

. Association of Official Analytical Chemists (AOAC). Official Methods of Analysis of the Association of Official Analytical Chemists 19th ed. AOAC lnc. Washington D.C., U.S.A. 2012

. A. Walkley and I. A. Black. An examination of degtiareff method for determining soil organic matter and apropos modification of the chromi acid titration method. Soil Science.1993; 37:29-38.

. R. Novozamsky, J. V. Eck, C. H. Shcouwenburg and V.I. Wallinga. Total nitrogen determination in plant material by means of the indophenol blue method. J. Agri Sci. 1974; 22:3-5.

. G. Tchobanoglous, H. Thesisen and S. Villal. Integrated solid waste management engineering principle and management Issues. McGraw-Hill, Inc. 1993, 798 p.

. K. W. Choi. Shelf cultivation of Oster Mushroom. In Mushroom Growr’s Hanbook Oster Mushroom cultivation. Mush world, 2004, p. 153-165.

. A. N. Philippoussis, P. A. Diamantopoulou, G.I. Zervakis. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes. World J Microbiol Biotechnol. 2003; 19: 551-557.

. R. Narain, R. K. Sahu, S. Kumar, S. K. Garg, C. S. Singh, and R. S. Kanaujia. Influence of different nitrogen rich supplements during cultivation of Pleurotus florida on maize cobs substrate. Environmentalist. 2008; 29:1-7.

. Z. Junior, L. Luiz, G. A. Linde and N. B. Colauto, N.B. Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method. Acta Sci Agron. 2010; 32:55-60.

. M. Moonmoon, M. N. Uddin, S. Ahmed, N.J. Shelly and M. A. Khan. Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on sawdust and rice straw in Bangladesh. Saudi J Biol Sci. 2010; 17:341-345.

. A. Tesfaw, A. Tadesse and G. Kiros. Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia. Appl Microbiol Biotechnol., 2015; 3:15-20.

. M. Mansour-Benamar, J. M. Savoie and L. Chavant, Valorization of solid olive mill wastes by cultivation of a local strain of edible mushrooms. C. R. Biol. 2013; 336:407-415.

. S. Sharma, R. K. P. Yadav and C. P. Pokhrel. Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. Pak J Biol Sci. 2013; 2:3-8.


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Last updated: 13 February 2019