Utilization of Paper-Cone Water Cups as an Alternative Lignocellulose Waste Substrate in Pleurotus ostreatus Production

Suvit SUWANNO; Aminoh AYAE; Nuttida SUWANNO

doi:10.48048/wjst.2019.3488

Authors

Suvit SUWANNO Faculty of Environmental Management, Prince of Songkla University, Songkhla 90112
Aminoh AYAE Faculty of Environmental Management, Prince of Songkla University, Songkhla 90112
Nuttida SUWANNO Faculty of Management Science, Prince of Songkla University, Songkhla 90112

DOI:

https://doi.org/10.48048/wjst.2019.3488

Keywords:

Organic waste utilization, Lignocellulosic waste, Bioconversion, Pleurotus ostreatus, Environmental management

Abstract

This study examined the utilization of paper-cone water cups as an alternative substrate for oyster mushroom (Pleurotus ostreatus) production. The research was conducted by using bioconversion technology and a profitable method for converting lignocellulosic residue from municipal solid waste into protein-rich biomass, thereby reducing waste paper and enhancing environmental quality. The most suitable substrate for mycelial growth was waste paper from paper-cone water cups (WPC) combined with rubber wood sawdust (RWS) at a 75%:25% dry basis ratio. The substrate mixture was adjusted to a moisture content of 70 %, and the C/N ratio was fixed at 20:1 by the addition of urea and supplementation with 8 % rice bran. Spawn running used 10 % seed inoculum. The mushrooms were cultivated on 500 g of substrate in polyvinyl chloride boxes (405 cm³) and incubated at 25 °C in the dark with the relative humidity maintained at 70 - 80 %. The fastest spawn running (mycelia development) occurred at 5^th days, with pin head formation at 9^th days and fruiting body formation at 12^th days.The highest yield recorded was 26.59g/100g.Under these conditions, the potential lignocellulosic waste conversion (biological efficiency) was recorded as 88.64 %, and the protein content of P. ostreatus was 35.75 % after 12 days of cultivation.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Packaging Intelligence Unit.Thailand Packaging Industry Market Reports.Available at: http://packaging.oie.go.th/new/search_manufacturing_data.php?ACTION=SEARCH, accessed May 2018.

A Sharp and J Sang-Arun. A Guide for Sustainable Urban Organic Waste Management in Thailand: Combining Food, Energy, and Climate Co-benefits. IGES Policy report. Asia-Pacific Network for Global Change Research (APN) Thailand, 2012, p. 17-43.

A Challcharoenwattana and C Pharino. Co-benefits of household waste recycling for local community’s sustainable waste management in Thailand. Sustainability 2015; 7, 7417-37.

C Chiemchairi, JP Juanga and C Visvanathan. Municipal solid waste management in Thailand and disposal emission inventory. Environ. Monit. Assess. 2007; 135, 13-20.

M Badu, SK Twumasi and NO Boadi. Effect of lignocellulosic in wood used as substrate on the quality yield of mushroom. Food Nutr. Sci. 2011; 2, 780-4.

T Chinda, N Leewattana and N Leeamnuayjaroen. The study of Landill sitable in Thailand. In: Proceedings of the 1st Mae Fah Lunng University International Conference, Chiang Rai, Thailand, 2012, p.1-8.

S Yildiz, ÜC Yildiz, ED Gezer and A Temiz. Some lignocellulosic wastes uses as raw material in cultivation of the Pleurotus ostreatus culture mushroom. Process. Biochem. 2002; 38, 301-6.

VP Mane, SS Patil, AA Syed and MMV Baig. Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju (Fr.) Singer. J. Zhejiang Univ. Sci. B 2007; 8, 745-51.

EA Adebayo and D Martinez-Carrera. Oyster mushrooms (Pleuurotus) are useful for utilizing lignocellulosic biomass. Afr. J. Biotech. 2015; 14, 52-67.

R Naraian, MP Singh and S Ram. Supplementation of basal substrate to boost up substrate strength and Oyster mushroom yield: An overview of substrates and supplements. Int. J. Curr. Microbiol. Appl. Sci. 2016; 5, 543-53.

R Zhang, X Li and JG Fadel. Oyster mushroom cultivation with rice and wheat straw. Bioresour. Tech. 2012; 82, 277-84.

M Dashtban, H Schrft and W Qin. Fungal bioconversion of lignocellulosic residues: Opportunities & perspectives. Int. J. Biol. Sci. 2009; 5, 578-95.

DJ Royse, J Baarsand and Q Tan. Current Overview of Mushroom Production in the World. In: DC Zied and A Pardo-Gimenez (eds.). Edible and Medicinal Mushrooms: Technology and Applications. Wiley, New York, 2017, p. 5-12.

LJLD Van-Griensven. Mushrooms as a Conventional Food Mushrooms: Conventional Food and Alternative Medicine. In: I Milenkovic and I MIlosavljevic (eds.). Mushroom Cultivation Manual for the Small Mushroom Entrepreneur. Eko-offungi, Belgrade, Serbia, 2017, p. 7-16.

JK Oloke and EA Adebayo. Effectveness of immunotherapies from oyster mushroom (Pleurotus species) in the management of immunocompromised patients. Int. J. Immunol. 2015; 3, 8-20.

A Gregori, M Svagelj and J Pohleve. Cultivation techniques and medicinal properties of Pleurotus spp. Food Tech. Biotech. 2007; 45, 238-49.

RP Tengerdy and G Szakacs. Bioconversion of lignocellulose in solid substrate fermentation. Biochem. Eng. J. 2003; 13, 169-79.

ST Chang and SP Wasser. The Cultivation and Environmental Impact of Mushrooms. Oxford Research encyclopedia of environmental science. Oxford University Press, Oxford, 2017.

AN Philippoussis, G Zervakis and P Diamantopoulou. Bioconversion of agricultural lignocllulosic waste through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World J. Microbiol. Biotech. 2001; 17, 191-200.

Q Mandeel, AAA Al-Laith and SA Mohamed. Cultivation on oyster mushroom (Pleurotus spp.) on various lignocellulosic wastes. World J. Microbiol. Biotech. 2005; 21, 601-7.

G Koutrotsios, KC Mountzouris, I Chatzipavlidis and GI Zervakis. Bioconversion of lignocellulosic residues by Agrocybe cylindracea and Pleurotus ostreatus mushroom fungi: Assessment of their effect on the final product and spent substrate properties. Food Chem. 2014; 61, 127-35.

R Sindnu, P Binod and A Pandey. Biological pretreatment of lignocellulosic biomass: An overview. Bioresour. Tech. 2016; 199, 76-82.

S Papong, C Yuvaniyama, P Lohsomboon and P Malakul. Overview of biomass utilization in Thailand. In: Proceedings of the Meeting for LCA in ASEAN Biomass Project, International Conference Center Bangkok, 2004.

S Yildiz, ÜC Yildiz, ED Gezer and A Temiz. Some lignocellulosic wastes uses as raw material in cultivation of the Pleurotus ostreatus culture mushroom. Process. Biochem. 202; 38, 301-6.

R Zhang, X Li and JG Fadel. Oyster mushroom cultivation with rice and wheat straw. Bioresour. Tech. 2002; 82, 277-84.

E Harada, T Morizono, T Sumiya and S Meguro. Production of andean-patagonic edible mushroom Grifola gargal on wood-based substrates. Mycoscience 2015; 56, 616-21.

E Baysal, H Peker, MM Yalinkiliҫ and A Temiz. Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresour. Tech. 2003; 89, 95-7.

Association of Official Analytical Chemists (AOAC). Official Methods of Analysis of the Association of Official Analytical Chemists. 19th ed. AOAC lnc. Washington DC, USA, 2012.

R Novozamsky, JV Eck, CH Shcouwenburg and VI Wallinga. Total nitrogen determination in plant material by means of the indophenol blue method. J. Agri. Sci. 1974; 22, 3-5.

A Walkley and IA Black. An examination of degtiareff method for determining soil organic matter and apropos modification of the chromic acid titration method. Soil Sci. 1934; 37, 29-38.

G Tchobanoglous, H Thesisen and S Vigil. Integrated Solid Waste Management Engineering Principles and Management Issues. McGraw-Hill, Singapore, 1993.

KW Choi. Shelf Cultivation of Oster Mushroom. Mushroom Growers Handbook 1 Oyster Cultivation. Mush World, 2004, p. 153-65.

PA Philippoussis, PA Diamantopoulou and GI Zervakis. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes. World J. Microbiol. Biotech. 2003; 19, 551-7.

D Salmones, M Gerardo, LM Raos and KN Waliszewski. Cultivation of Shitatake mushroom, Lentimula edodes in several lignocellulosic materials originating from the tropics. Agronomie 1999; 19, 13-9.

M Obodia, J Cleland-Okine and KA Vowotor. Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. J. Ind. Microbiol. Biot. 2003; 30, 146-9.

EA Adebayo and D Martiinez-Carrera. Oster mushroom (Pleurotus) are useful for utilizing lignocellulosic biomass. Afr. J. Biotech. 2015; 14, 52-67.

Z Junior, L Luiz, GA Linde and NB Colauto. Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method. Acta Sci. Agron. 2010; 32, 55-60.

S Rezaean and H Pourianfar. A comparative study on bioconversion of different agro wastes by wild and cultivated strains of Flammulina velutipes. Waste Biomass Valori. 2016; 8, 2631-42.

M Moonmoon, MN Uddin, S Ahmed, NJ Shelly and Md A Khan. Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on sawdust and rice straw in Bangladesh. Saudi J. Biol. Sci. 2010; 17, 341-5.

A Tesfaw, A Tadesse and G Kiros. Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia. Appl. Microbiol. Biotech. 2015; 3, 15-20.

R Naraian, RK Sahu, S Kumar, SK Garg, CS Singh and RS Kanaujia. Inﬂuence of different nitrogen rich supplements during cultivation of Pleurotus ﬂorida on corncob substrate. Environmentalist 2009; 29, 1-7.

M Mansour-Benamar, JM Savoie and L Chavant. Valorization of solid olive mill wastes by cultivation of a local strain of edible mushrooms. Comptes Rendus Biol. 2013; 336, 407-15.

MI Bhatti, MM Jiskani, KH Wagan, MA Pathan and MR Magsi. Growth, development and yield of oyster mushroom, Pleuotus ostreatus (JACQ. EX. FR.) Kummer as affected by different spawn rates. Pak. J. Bot. 2007; 39, 2685-92.

S Sharma, RPK Yadav and CP Pokhrel. Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. J. New Biol. Rep. 2013; 2, 3-8.