Potentiating Effect of Oxymatrine and 5-fluorouracil on Cell Survival and Inhibition of Cancer Metastasis in SW-620 Colorectal Cancer Cells

Suwisit MANMUAN; Punyo YOYKAEW; Piyachat THUETONG; Patchraporn ASIPONG; Nattanicha RIANTONG

doi:10.48048/wjst.2018.3388

Authors

Suwisit MANMUAN Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131
Punyo YOYKAEW Doctor of Pharmacy Program, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131
Piyachat THUETONG Doctor of Pharmacy Program, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131
Patchraporn ASIPONG Doctor of Pharmacy Program, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131
Nattanicha RIANTONG Doctor of Pharmacy Program, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131

DOI:

https://doi.org/10.48048/wjst.2018.3388

Keywords:

Oxymatrine, cell viability, cell invasion, colorectal cancer, 5-fluorouracil

Abstract

Colorectal cancer (CRC) is the most common cancer in the world, beginning in the cell lining of the colon and rectum. 5-fluorouracil (5-FU) is a first-line therapy for colorectal cancer patients. However, the response rate of 5-FU in advanced colorectal cancer patients is only 10 - 15 %, and can fail due to drug resistance. Therefore, the development of a new anticancer compound for improving the response rate or for reversing resistance to 5-FU is urgently needed. Oxymatrine is a major component of Sophora flavescens. Recently, many pharmacological effects have been exhibited. The objectives of the present study were to investigate the anticancer activity of oxymatrine, as well as to potentiate the effect of oxymatrine with 5-FU on cell viability, colony-forming, cell migration, cell invasion, and determined MMP-9 protein expression in SW-620 colorectal cancer cells. The results demonstrated that oxymatrine significantly inhibited the cell viability of SW-620 cells after 24, 48, and 72 h treatments. Oxymatrine and 5-FU interaction was synergistically greater in inhibiting the cell survival of SW-620 cells than 5-FU was alone. Oxymatrine also decreased colony formation, cell migration, and cell invasion through reducing the level of MMP-9 protein in SW-620 cells. These first findings elucidated an efficacious anticancer agent, which is derived from natural sources, which could be used to overcome 5-FU resistance in colorectal cancer, and may be beneficial for patients with colorectal cancer who are treated with 5-FU.

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