Adhesion Conditions of Bifidobacterium pseudocatenulatum KAKii to Human Enterocyte-like Caco-2 Cell Lines.

Mizanurfakhri GHAZALI, Nurul Wahida SHOKHIMI, Mazatulikhma MAT ZAIN, Khalilah ABDUL KHALIL

Abstract


Attachment ability of bifidobacteria strains to human intestinal surface is one of an important criterion as probiotic candidate. However, the attachment activity is influence by external and internal conditions. The study was conducted to screened cell surface hydrophobicity and adhesion score of bifidobacteria strains. Attachment conditions (pH and exposure time) of selected strain to human enterocyte-like Caco-2 cell lines were investigated subsequently. Three different solvents (n-hexadecane, Toluene and Xylene) were used in cell surface hydrophobicity analysis. Based on the result obtained, it shows that xylene has presented consistence cell hydrophobicity activity in all strains used. Bifidobacterium pseudocatenulatum KAKii (wild type strain) had gave a promising cell hydrophobicity activity with no significant difference (p>0.05) when compared to Lactobacillus plantarum NBRC 3070 with xylene as a solvent and also presented a significant higher attachment score (p<0.05) compared to all strains used. The influence of pH and time exposure B. pseudocatenulatum KAKii to Caco-2 cells had revealed that this strain was favor to attach to the intestinal cell line at pH 6 and after 120 minutes of exposure. Further optimization of attachment conditions will be carried out.  


Keywords


Bifidobacterium strains, cell-surface hydrophobicity, pH conditions, time of exposure, Caco-2 cell lines

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