Adhesion Conditions of Bifidobacterium pseudocatenulatum KAKii to Human Enterocyte-like Caco-2 Cell Lines


  • Mizanurfakhri GHAZALI Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
  • Nurul Wahida SHOKHIMI Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
  • Mazatulikhma MAT ZAIN Institute of Science, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
  • Khalilah ABDUL KHALIL Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia


Bifidobacterium strains, cell-surface hydrophobicity, pH conditions, time of exposure, Caco-2 cell lines


Attachment ability of bifidobacteria strains to the human intestinal surface is an important criterion as a probiotic candidate. However, attachment activity is influenced by external and internal conditions. This study was conducted to screen cell surface hydrophobicity and adhesion scores of bifidobacteria strains. Attachment conditions (pH and exposure time) of selected strains to human enterocyte-like Caco-2 cell lines were subsequently investigated. Three different solvents (n-hexadecane, Toluene, and Xylene) were used in cell surface hydrophobicity analysis. Based on the results obtained, xylene presented consistent cell hydrophobicity activity in all strains used. Bifidobacterium pseudocatenulatum KAKii (wild type strain) gave promising cell hydrophobicity activity with no significant difference (p > 0.05) when compared to Lactobacillus plantarum NBRC 3070 with xylene as a solvent, and also presented a significantly higher attachment score (p < 0.05) compared to all strains used. The influence of pH and time exposure on adhesion of B. pseudocatenulatum KAKii to Caco-2 cells revealed that this strain was favored to attach to the intestinal cell line at pH 6 and after 120 min of exposure. Further optimization of attachment conditions will be carried out.


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QS Ali, AJ Farid, BM Kabeir, S Zamberi, M Shuhaimi, HM Ghazali and AM Yazid. Adhesion properties of Bifidobacterium pseudocatenulatum G4 and Bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. Intern. J. Biol. Life Sci. 2007; 3, 4. 266-70.

M Candela, E Biagi, M Centanni, S Turroni, M Vici, F Musiani, B Vitali, S Bergman, S Hammerschmidt and P Brigidi. Bifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host. Microbiology 2009; 155, 3294-303.

M Ventura, MO Connell-Motherway, S Leahy, JA Moreno-Munoz, GF Fitzgerald and D van Sinderen. From bacterial genome to functionality: Case Bifidobacteria. Intern. J. Food Microbiol. 2007; 120, 2-12.

B Wang, H Wei, J Yuan, Y Li, N Li and J Li. Identification of a surface protein from Lactobacillus reuteri JCM1081 that adheres to porcine gastric mucin and human enterocyte-like HT-29 cells. Curr. Microbiol. 2008; 57, 33-8.

S Guglielmetti, I Tamagnini, D Mora, M Minuzzo, A Scarafoni, S Ariolli, J Hellman, M Karp and C Parini. Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cells. Appl. Environ. Mirobiol. 2008; 74, 4695-702.

L Ruiz, Y Coute, B Sanchez, CG Reyes-Gavilan, JC Sanchez and A Margolles. The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment. Microbiology 2009; 155, 957-67.

L Shakirova, M Grube, M Gavare, L Auzina and P Zikmanis. Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 cell surface hydrophobicity and survival of the cells under adverse environmental conditions. J. Ind. Microbiol. Biotechnol. 2013; 40, 85-93.

M Rosenberg, D Gutnick and E Rosenberg. Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity. FEMS Microbiol. Lett. 1980; 9, 29-33.

RK Duary, YS Rajput, VK Batish and S Grover. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Ind. J. Med. Res. 2011; 134, 664-71.

MC Ahumada, E Bru, ME Colloca, ME Lopez and ME Nader-Macias. Evaluation and comparison of lactobacilli characteristics in the mouths of patients with or without cavities. J. Oral Sci. 2003; 45, 1-9.

M Chichlowski, G De Lartigue, JB German, HE Raybould and DA Mills. Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affects intestinal epithelial function. J. Pediatr. Gastroent. Nut. 2012; 55, 321-7.

R Bhadekar, G Dixit, D Samarth and V Tale. Comparative studies on potential probiotic characteristics of Lactobacillus acidophilus strains. Eurasia. J. Biosci. 2013; 7, 1-9.

TA Piekarczyk, AK Baginska and J Bardowski. Genome sequence of the probiotic strain Lactobacillus rhamnosus (formerly Lactobacillus casei) LOCK900. Genome Announc. 2013; 1, e00640-13.

K Bath, S Roos, T Wall and H Jonsson. The cell surface of Lactobacillus reuteri ATCC 55730 highlighted by identification of 126 extracellular proteins from the genome sequence. FEMS Microbiol. Lett. 2006; 253, 75-82.

Y Farzini, MN Rohana and AK Khalilah. Survivability Characteristics of Bifidobacterium sp. Isolates from New Borne Muconium and Breast Fed/Formulated Infant Faeces in Acidic-simulated Intestinal Conditions. In: MFF Abdullah, MTB Ali and FZM Yusof. (Eds.). Bioresources Technology in Sustainable Agriculture. Taylor and Francis Group, 2016.

M Lewandoska, A Olejnik, M Neumann, A Krepulec, J Piotrowska, A Teresiak and W Grajek. Comparative in vitro study on the adhesion of the probiotic and pathogenic bacteria to different human intestinal cell line. Biotechnologia 2005; 2, 215-33.

R Champana, SV Hemert and W Baffone. Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. Gut Pathog. 2017; 9, 12.

AA Abdulla, TA Abed and AM Saeed. Adhesion, autoaggregation and hydrophobicity of six Lactobacillus strains. Brit. Microbiol. Res. J. 2014; 4, 381-91.

A Orlowski and M Bielecka. Preliminary characteristics of Lactobacillus and Bifidobacterium strains as probiotic candidates. Pol. J. Food Nutr. Sci. 2006; 3, 269-75.

L Shakirova, L Auzina, P Zikmanis, M Gavare and M Grube. Influence of growth conditions on hydrophobicity of Lactobacillus acidophilus and Bifidobacterium lactis cells and characteristics by FT-IR spectra. Spectromet 2010; 24, 251-5.

RI Gonzalez, B Sanchez, L Ruiz, F Turoni, M Ventura, MP Ruas, M Gueimonde and A Margolles. Role of extracellular transaldolase from Bifidobacterium bifidum in mucin adhesion and aggregation. Appl. Environ. Microbiol. 2012; 78, 3992-8.




How to Cite

GHAZALI, M., SHOKHIMI, N. W., MAT ZAIN, M., & ABDUL KHALIL, K. (2018). Adhesion Conditions of Bifidobacterium pseudocatenulatum KAKii to Human Enterocyte-like Caco-2 Cell Lines. Walailak Journal of Science and Technology (WJST), 17(2), 96-102. Retrieved from