Adhesion Conditions of Bifidobacterium pseudocatenulatum KAKii to Human Enterocyte-like Caco-2 Cell Lines

Authors

  • Mizanurfakhri GHAZALI Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
  • Nurul Wahida SHOKHIMI Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
  • Mazatulikhma MAT ZAIN Institute of Science, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia
  • Khalilah ABDUL KHALIL Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia

Keywords:

Bifidobacterium strains, cell-surface hydrophobicity, pH conditions, time of exposure, Caco-2 cell lines

Abstract

Attachment ability of bifidobacteria strains to the human intestinal surface is an important criterion as a probiotic candidate. However, attachment activity is influenced by external and internal conditions. This study was conducted to screen cell surface hydrophobicity and adhesion scores of bifidobacteria strains. Attachment conditions (pH and exposure time) of selected strains to human enterocyte-like Caco-2 cell lines were subsequently investigated. Three different solvents (n-hexadecane, Toluene, and Xylene) were used in cell surface hydrophobicity analysis. Based on the results obtained, xylene presented consistent cell hydrophobicity activity in all strains used. Bifidobacterium pseudocatenulatum KAKii (wild type strain) gave promising cell hydrophobicity activity with no significant difference (p > 0.05) when compared to Lactobacillus plantarum NBRC 3070 with xylene as a solvent, and also presented a significantly higher attachment score (p < 0.05) compared to all strains used. The influence of pH and time exposure on adhesion of B. pseudocatenulatum KAKii to Caco-2 cells revealed that this strain was favored to attach to the intestinal cell line at pH 6 and after 120 min of exposure. Further optimization of attachment conditions will be carried out.

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Published

2018-11-05

How to Cite

GHAZALI, M., SHOKHIMI, N. W., MAT ZAIN, M., & ABDUL KHALIL, K. (2018). Adhesion Conditions of Bifidobacterium pseudocatenulatum KAKii to Human Enterocyte-like Caco-2 Cell Lines. Walailak Journal of Science and Technology (WJST), 17(2), 96-102. Retrieved from http://wjst.wu.ac.th/index.php/wjst/article/view/3258